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Transformation of Tomato (Lycopersicon esculentum Mill.) for Virus Disease Protection

  • H. Toyoda
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

One of the major aims of plant biotechnology is to protect crop plants from diseases. Transforming plants with certain genes relating to disease resistance would be a direct and profitable strategy for producing transgenic disease-resistant plants. For completing this strategy, however, it would be important and may be essential to evaluate in advance the feasibility of a gene to be used for transformation. From this point of view, this chapter first describes an efficient system for transforming tomato callus cells to elucidate at a cellular level the effectiveness of transformation with respect to virus disease protection. A microinjection technique has been used for this purpose, since it has been well recognized that this technique is a reliable method for directly introducing foreign genes into the correct portions of target cells (Neuhaus and Spangenberg 1990; Potrykus 1990). Nevertheless, this system may not be necessarily effective for an efficient production of transgenic plants from transformed cells due to its complicated procedures and difficulty in plant regeneration. In order to promote and simplify production of transgenic plants, this chapter also describes an Agrobacterium-mediated gene transfer for an actual genetic transformation of tomato.

Keywords

Hairy Root Coat Protein Tobacco Mosaic Virus Coat Protein Gene Agrobacterium Rhizogenes 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • H. Toyoda
    • 1
  1. 1.Faculty of AgricultureKinki UniversityNaraJapan

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