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Transformation in Muskmelon (Cucumis Melo L.)

  • G. Fang
  • R. Grumet
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 23)

Abstract

Muskmelon (Cucumis melo L.), a tropical species that probably originated in Africa(Whitaker and Davis 1962), has become an important crop throughout the world. FAO statistics indicate that approximately 600 000 ha of melons was produced worldwide in 1988. Muskmelons are a high value crop, averaging approximately $7600/ha in the United States (USDA Agricultural Statistics Service). Although muskmelons are primarily eaten fresh as a dessert, they can be used in a variety of ways (McCreight et al. in press): immature fruits are consumed fresh, cooked, or pickled; mature fruits are canned, used for syrup, jam or juice, or eaten fresh; and the seeds are a source of unsaturated vegetable oil and protein. Muskmelon seeds and roots also have been used for medicinal purposes as an emetic (Schultes 1990).

Keywords

Zucchini Yellow Mosaic Virus Cocultivation Period Melon Genome Agrobacterium Concentration Muskmelon Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bajaj VPS (ed) (1989) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New YorkGoogle Scholar
  2. Beachy RN, Loesch-Fries S, Turner NE (1990) Coat protein-mediated resistance against virus infection. Annu Rev Phytopathol 28:451–474CrossRefGoogle Scholar
  3. Ben Tahar S, De Both M, Thion L (1989) Regeneration and transformation of muskmelon. In: Thomas CE (ed) Proc Cucurbitaceae 89: evaluation and enhancement of cucurbit germplasm. Charleston, SC, pp 21–27Google Scholar
  4. Chyi YS, Jorgensen AJ, Goldstein D, Tanksley SD, Loaiza-Fifueroa (1986) Location and stability of Agrobacterium-med’mted T-DNA insertions in the Lycopersicon genome. Mol Gen Genet 204:64–69CrossRefGoogle Scholar
  5. Dellaporta SL, Wood J, Hicks JB (1985) Maize DNA miniprep. In: Malmberg R, Messing J, Sussex I (eds) Molecular biology of plants. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York pp 36–37Google Scholar
  6. Dong JZ, Yang MZ, Jia SR, Chua NH (1991) Transformation of melon and expression from the cauliflower mosaic virus 35S promoter in transgenic melon plants. Bio/Technol 9:858–863CrossRefGoogle Scholar
  7. Fang G, Grumet R (1990) Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. Plant Cell Rep 9:160–164CrossRefGoogle Scholar
  8. Fang G, Hammar S, Grumet R (1992) A quick and inexpensive method for removing polysaccharides from genomic DNA. BioTechniques 13:52–55PubMedGoogle Scholar
  9. Gasser CS, Fraley RT (1989) Genetically engineering plants for crop improvement. Science 244:1293–1299PubMedCrossRefGoogle Scholar
  10. Godwin I, Todd G, Ford-Lloyd B, Newbury HJ (1991) The effects of acetosyringone and pH on Agrobacterium-medmted transformation vary according to plant species. Plant Cell Rep 9:671–675CrossRefGoogle Scholar
  11. Gonsalves CV, Xue B, Namba S, Ling K, Slightom JL, Gonsalves D (1991) Transgenic muskmelon with the coat protein gene of the white leaf strain of cucumber mosaic virus. Phytopathology Abstr 295.Google Scholar
  12. Grumet R (1990) Genetically engineered plant virus resistance. HortScience 25:508–513Google Scholar
  13. Grumet R, Fang G (1990) cDNA cloning and sequence analysis of the 3’ terminal region of zucchini yellow mosaic virus RNA. J Gen Virol 71:1619–1622Google Scholar
  14. Kathal R, Bhatnagar SP, Bhojwani SS (1988) Regeneration of plants from leaf explants of Cucumis melo L. Plant Cell Rep 7:449–451Google Scholar
  15. McCreight JD, Nerson H, Grumet R. Muskmelon (Cucumis melo L.). In: Kallo G Bergh BO (eds) Genetic improvement of vegetable crops. Pergamon, Oxford (in press)Google Scholar
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  17. Nameth ST, Laemmlen FF, Dodds JA (1985) Viruses cause heavy melon losses in desert valleys. Calif Agric (July):28–29Google Scholar
  18. Niedz RP, Smith SS, Dunbar KB, Stephens CT, Murakishi HH (1989) Factors influencing shoot regeneration from cotyledonary explants of Cucumis melo. Plant Cell Tissue Organ Cult 18:313–319CrossRefGoogle Scholar
  19. Rothstein SJ, Lahners KN, Lotstein RJ, Carozzi NB, Jayne SM, Rice DA (1987) Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation. Gene 53:153–161CrossRefGoogle Scholar
  20. Schultes RE (1990) Biodynamic cucurbits in the New World tropics. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of the Cucurbitaceae. Comstock, Ithaca, New York, pp 307–317Google Scholar
  21. Whitaker TW, Davis GN (1962) Cucurbits: botany, cultivation and utilization. Hill, LondonGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • G. Fang
  • R. Grumet
    • 1
  1. 1.Horticulture DepartmentMichigan State UniversityEast LansingUSA

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