Regeneration of Plants from Protoplasts of Lotus spp. (Birdsfoot trefoil)
There are approximately 200 species of Lotus distributed throughout the world (Grant 1965). The greatest diversity occurs in the Mediterranean basin, considered the regional center of origin. Lotus corniculatus L. (birdsfoot trefoil) is native to Europe and parts of Asia. Nineteenth-century reports show that it grew naturally in many pastures and was a good feed for cattle and horses. However, it was not until after 1900 that it was cultivated in Europe (MacDonald 1946).
KeywordsPlant Regeneration Somatic Hybrid Protoplast Isolation Protoplast Culture Forage Legume
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