Regeneration of Plants from Protoplasts of Brassica alboglabra Bailey (Chinese Kale)
The genus Brassica comprises a wide range of crop plants with great economic value worldwide. Brassica alboglabra Bailey (Chinese kale or Kailan, 2n = 18) (Fig. 1), which is believed to have originated from the southern part of China, is an important vegetable crop in Southeast Asia. In recent years, major efforts have focused on the utilization of protoplast technology for Brassica improvement via somatic hybridization or cell transformation, and considerable progress has been made in some species including B. napus, B. oleracea, and B. juncea (Schenck and Hoffmann 1984; Pua 1990, Thomzik 1993). However, work on protoplast culture of B. alboglabra has been limited, although plant regeneration from various cultured expiants, e.g., cotyledon, hypocotyl, and stem, has been reported (Zee and Hui 1977; Wong and Loh 1987; Pua et al. 1989).
KeywordsPlant Regeneration Brassica Juncea Mesophyll Protoplast Protoplast Culture Plant Cell Tissue Organ Cult
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