Advertisement

Transformation in Hordeum vulgare L. (Barley)

  • Hideyoshi Toyoda
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 22)

Abstract

Genetic transformation of graminaceous plants has been mainly conducted by directly introducing foreign genes into their protoplasts by means of electroporation of polyethylene glycol treatment. In these methods, plant regeneration from protoplasts is a prerequisite for producing transgenic plants. However, the inability of protoplasts of many monocotyledonous plants to regenerate into plants makes it difficult to generalize these methods in a broad range of monocotyledonous species. Therefore, the substitutive method such as a microprojectile bombardment has been developed and applied to direct delivery of foreign genes into monocotyledonous plant materials. Recently the author reported the transient expression of foreign gene introduced into barley coleoptile cells by microinjection and discussed the possible application of coleoptile tissues to a production of transgenic barley (Toyoda et al. 1990; 1992). From this point of view, this chapter describes (1) callus induction and plant regeneration from coleoptile tissues in order to complete a coleoptile system of barley, (2) verification of expression of foreign genes introduced into coleoptile cells and (3) possibility of production of disease-resistant barley. The progress of these investigations would make it possible to produce transgenic plants in a broad range of monocotyledonous species in which protoplast regeneration is difficult.

Keywords

Powdery Mildew Callus Induction Tobacco Mosaic Virus Foreign Gene Cytoplasmic Streaming 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boller T (1987) Hydrolytic enzymes in plant disease resistance. In: Kosuge T, Nester EW (eds) Plantmicrobe interactions: Molecular and genetic perspectives, vol. 2. Macmillan, New York, pp 385–413.Google Scholar
  2. Bushneil WR, Dueck J, Rowell JB (1967) Living haustoria and hyphae of Erysiphe graminis f. sp. hordei with intact and partly dissected host cells of Hordeum vulgare. Can J Bot 45:1179–1732CrossRefGoogle Scholar
  3. Childs GV, Lloyd JM, Unabia G, Gharib SD, Wierman ME, Chin WW (1987) Detection of leutinizing hormone β messenger ribonucleic acid (RNA) in individual gonadotropes after castration: use of a new in situ hybridization method with a photobiotinylated complementary RNA probe. Mol Endocrinol 1:926–932.PubMedCrossRefGoogle Scholar
  4. Davis LL, Bartnicki-Garcia S (1984) Chitosan synthesis by the tandem action of chitin synthetase and chitin deacetylase from Mucor rouxii. Biochemistry 23:1065–1073.CrossRefGoogle Scholar
  5. Forster AC, Mclnnes JL, Skingle DC, Symons RH (1985) Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucelic Acid Res 13:745–761.CrossRefGoogle Scholar
  6. Fukamizo T, Sonoda K, Toyoda H, Ouchi S, Goto S (1990) Solid-state 13C-NMR analysis of cell wall components of Fusarium oxysporum. Agric Biol Chem 54:2761–2762.CrossRefGoogle Scholar
  7. Goodman RN, Kiraly Z, Wood KR (1986) The biochemistry and physiology of plant disease. Univ Missouri Press, Columbia, pp 433.Google Scholar
  8. Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25:263–269.PubMedCrossRefGoogle Scholar
  9. Hoefler H, Childers H, Montminy MR, Lechan RM, Goodman RH, Wolfe HJ (1986) In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA probes. Histochemical J 18:597–604.CrossRefGoogle Scholar
  10. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405.CrossRefGoogle Scholar
  11. Jelaska S, Rengel Z, Cesar V (1984) Plant regeneration from mesocotyl callus of Hordeum vulgare L. Plant Cell Rep 3:125–129.CrossRefGoogle Scholar
  12. Matsuda Y, Toyoda H, Ouchi S (1989a) Application of microinjection to appressoria and haustoria of Erysiphe graminis f sp. hordei. Ann Phytopathol Soc Jpn 55:67–68.CrossRefGoogle Scholar
  13. Matsuda Y, Toyoda H, Nishiguchi T, Ouchi S (1989b) Direct introduction of fluorescein isothiocyanate-conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici by electroporation. J Phytopathol 125:89–96.CrossRefGoogle Scholar
  14. Melton DA (1984) Injected anti-sense RNAs specifically block messenger RNA translation in vivo. Proc Natl Acad Sci USA 82:144–148.CrossRefGoogle Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.CrossRefGoogle Scholar
  16. Potrykus I (1990) Gene transfer to cereals: an assessment. Bio/Technol 8:535–542.CrossRefGoogle Scholar
  17. Raikhel NV, Bendnarek SY, Lerner DR (1989) In situ RNA hybridization in plant tissue. In: Gelvin SB, Schilperort RA, Verma DPS (eds) Plant moleucular biology manual. Kluwer, Dordrecht, pp B9:1-32.Google Scholar
  18. Rengel Z, Jelaska S (1986) Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L. J Plant Physiol 124:385–392.Google Scholar
  19. Shiraishi T, Ouchi S, Oku H (1976) Chitin component in haustorial wall of powdery mildew fungus of barley. Sci Rep Fac Agric Okayama Univ 47:21–24.Google Scholar
  20. Swegle M, Hunga J-K, Lee G, Muthukrishnan S (1989) Identification of an endochitinase cDNA clone from barley aleurone cells. Plant Mol Biol 12:403–412.CrossRefGoogle Scholar
  21. Toyoda H, Matsuda Y, Hirai T (1985) Resistance mechanism of cultured plant cells to tobacco mosaic virus (III) Efficient microinjection of tobacco mosaic virus into tomato callus cells. Ann Phytopathol Soc Jpn 51:32–38.CrossRefGoogle Scholar
  22. Toyoda H, Matsuda Y, Hirai T (1986) Multiplication and translocation of tobacco mosaic virus microinjected into cell-aggregates of tomato callus. Plant Tissue Cult Lett 3:22–27.CrossRefGoogle Scholar
  23. Toyoda H, Matsuda Y, Shoji R, Ouchi S (1987) A microinjection technique for conidia of Erysiphe graminis f. sp. hordei. Phytopathology 77:815–818.CrossRefGoogle Scholar
  24. Toyoda H, Matsuda Y, Utsumi R, Ouchi S (1988) Intranuclear microinjection for transformation of tomato callus cells. Plant Cell Rep 7:293–296.CrossRefGoogle Scholar
  25. Toyoda H, Oki T, Matsuda Y, Katsuragi K, Nishiguchi T, Ouchi S (1989) Transformation of constituent cells of tomato callus aggregates by intranuclear microinjection. Plant Tissue Cult Lett 6:95–97.CrossRefGoogle Scholar
  26. Toyoda H, Yamaga T, Matsuda Y, Ouchi S (1990a) Transient expression of the β-glucuronidase gene introduced into barley coleoptile cells by microinjection. Plant Cell Rep 9:299–302.CrossRefGoogle Scholar
  27. Toyoda H, Koura Y, Matsuda Y, Horikoshi Y, Tamai T, Yamaga T, Ikeda S, Shimabayashi M, Monta M, Ouchi S (1990b) Callus induction and plant regeneration from barley coleoptile tissues. Plant Tissue Cult Lett 7:202–204.CrossRefGoogle Scholar
  28. Toyoda H, Matsuda Y, Yamaga T, Ikeda S, Morita M, Tamai T, Ouchi S (1991) Suppression of the powdery mildew pathogen by chitinase microinjected into barley coleoptile epidermal cells. Plant Cell Rep 10:217–220.Google Scholar
  29. Toyoda H, Katsuragi K, Tamai R, Ouchi S (1991b) DNA sequence of genes for detoxification of fusaric acid, a wilt-inducing agent produced by Fusarium species. J. Phytopathol 133:265–277.CrossRefGoogle Scholar
  30. Toyoda H, Matsuda Y, Nogi Y, Tamai T, Ouchi S (1992) An improved system for foreign gene expression in barley coleoptile epidermis: gene transfer and in situ detection of gene products by dual microinjection. Plant Tissue Cult Lett 9:154–163.CrossRefGoogle Scholar
  31. Yamamoto F, Furusawa M, Furusawa I, Obinata M (1982) The “pricking” method. A new efficient technique for mechanically introducing foreign DNA into the nuclei of culture cells. Exp Cell Res 142:79–84.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Hideyoshi Toyoda
    • 1
  1. 1.Faculty of AgricultureKinki UniversityNara 631Japan

Personalised recommendations