Transformation in Hordeum vulgare L. (Barley)
Genetic transformation of graminaceous plants has been mainly conducted by directly introducing foreign genes into their protoplasts by means of electroporation of polyethylene glycol treatment. In these methods, plant regeneration from protoplasts is a prerequisite for producing transgenic plants. However, the inability of protoplasts of many monocotyledonous plants to regenerate into plants makes it difficult to generalize these methods in a broad range of monocotyledonous species. Therefore, the substitutive method such as a microprojectile bombardment has been developed and applied to direct delivery of foreign genes into monocotyledonous plant materials. Recently the author reported the transient expression of foreign gene introduced into barley coleoptile cells by microinjection and discussed the possible application of coleoptile tissues to a production of transgenic barley (Toyoda et al. 1990; 1992). From this point of view, this chapter describes (1) callus induction and plant regeneration from coleoptile tissues in order to complete a coleoptile system of barley, (2) verification of expression of foreign genes introduced into coleoptile cells and (3) possibility of production of disease-resistant barley. The progress of these investigations would make it possible to produce transgenic plants in a broad range of monocotyledonous species in which protoplast regeneration is difficult.
KeywordsPowdery Mildew Callus Induction Tobacco Mosaic Virus Foreign Gene Cytoplasmic Streaming
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- Boller T (1987) Hydrolytic enzymes in plant disease resistance. In: Kosuge T, Nester EW (eds) Plantmicrobe interactions: Molecular and genetic perspectives, vol. 2. Macmillan, New York, pp 385–413.Google Scholar
- Childs GV, Lloyd JM, Unabia G, Gharib SD, Wierman ME, Chin WW (1987) Detection of leutinizing hormone β messenger ribonucleic acid (RNA) in individual gonadotropes after castration: use of a new in situ hybridization method with a photobiotinylated complementary RNA probe. Mol Endocrinol 1:926–932.PubMedCrossRefGoogle Scholar
- Goodman RN, Kiraly Z, Wood KR (1986) The biochemistry and physiology of plant disease. Univ Missouri Press, Columbia, pp 433.Google Scholar
- Raikhel NV, Bendnarek SY, Lerner DR (1989) In situ RNA hybridization in plant tissue. In: Gelvin SB, Schilperort RA, Verma DPS (eds) Plant moleucular biology manual. Kluwer, Dordrecht, pp B9:1-32.Google Scholar
- Rengel Z, Jelaska S (1986) Somatic embryogenesis and plant regeneration from seedling tissues of Hordeum vulgare L. J Plant Physiol 124:385–392.Google Scholar
- Shiraishi T, Ouchi S, Oku H (1976) Chitin component in haustorial wall of powdery mildew fungus of barley. Sci Rep Fac Agric Okayama Univ 47:21–24.Google Scholar
- Toyoda H, Matsuda Y, Yamaga T, Ikeda S, Morita M, Tamai T, Ouchi S (1991) Suppression of the powdery mildew pathogen by chitinase microinjected into barley coleoptile epidermal cells. Plant Cell Rep 10:217–220.Google Scholar