Transformation in Hordeum vulgare L. (Barley)

  • Hideyoshi Toyoda
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 22)


Genetic transformation of graminaceous plants has been mainly conducted by directly introducing foreign genes into their protoplasts by means of electroporation of polyethylene glycol treatment. In these methods, plant regeneration from protoplasts is a prerequisite for producing transgenic plants. However, the inability of protoplasts of many monocotyledonous plants to regenerate into plants makes it difficult to generalize these methods in a broad range of monocotyledonous species. Therefore, the substitutive method such as a microprojectile bombardment has been developed and applied to direct delivery of foreign genes into monocotyledonous plant materials. Recently the author reported the transient expression of foreign gene introduced into barley coleoptile cells by microinjection and discussed the possible application of coleoptile tissues to a production of transgenic barley (Toyoda et al. 1990; 1992). From this point of view, this chapter describes (1) callus induction and plant regeneration from coleoptile tissues in order to complete a coleoptile system of barley, (2) verification of expression of foreign genes introduced into coleoptile cells and (3) possibility of production of disease-resistant barley. The progress of these investigations would make it possible to produce transgenic plants in a broad range of monocotyledonous species in which protoplast regeneration is difficult.


Powdery Mildew Callus Induction Tobacco Mosaic Virus Foreign Gene Cytoplasmic Streaming 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Hideyoshi Toyoda
    • 1
  1. 1.Faculty of AgricultureKinki UniversityNara 631Japan

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