Advertisement

Regeneration of Plants from Protoplasts of Pyrus spp. (Pear)

  • S. J. Ochatt
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 22)

Abstract

The earliest record of pear cultivation in Europe is that provided by Homer who, around 1000 B.C., wrote that pears were “one of the gifts of the gods”. By circa 300 B.C., pear cultivation was well established in Greece with distinct cultivars propagated by grafting and cuttings. France and Belgium were the main centers for pear improvement during its main developmental phase (1750–1850), where the fruit characters (with the exception of texture) found in today’s commercially grown cultivars were established. Several of the cultivars which are now among the most economically important globally [e.g., Williams’ Bon Chrétien (syn. Bartlett), Conference, etc.] developed at this time, and were derived from selections among open-pollinated seedlings of common cultivars (Vavrà and Orel 1971).

Keywords

Somatic Hybridization Mesophyll Protoplast Fire Blight Protoplast Viability Wild Pear 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brisset MN, Ochatt SJ, Paulin JP (1990) Evidence for quantitative responses during co-culture of Pyrus communis protoplasts and Erwinia amylovora. Plant Cell Rep 9:272–275.CrossRefGoogle Scholar
  2. Layne REC, Quamme HA (1975) Pears. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue University Press, West Lafayette, Indiana: 38–70.Google Scholar
  3. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.CrossRefGoogle Scholar
  4. Ochatt SJ (1987) Coltura di protoplasti come metodo per il miglioramento genetico nelle piante da frutto. Frutticoltura 49:58–60.Google Scholar
  5. Ochatt SJ (1990) Protoplast technology and top-fruit tree breeding. Acta Hortic 280:215–226.Google Scholar
  6. Ochatt SJ (1993) Regeneration of plants from protoplasts of some stone fruits (Prunus spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 23: Plant protoplasts and genetic engineering IV. Springer Berlin Heidelberg pp 78–96.Google Scholar
  7. Ochatt SJ Caso OH (1986) Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var. pyraster L.). J Plant Physiol 122:243–249.Google Scholar
  8. Ochatt SJ, Patat-Ochatt EM (1990) Assessments of graft-compatibility of somatic hybrids, Pyrus communis var. pyraster L (+) Prunus avium x pseudocerasus, and species of the subfamilies Pomoideae and Prunoideae, Rosaceae. VII Int Cong Plant Tissue and Cell Culture, Amsterdam: 216.Google Scholar
  9. Ochatt SJ, Patat-Ochatt EM (1991) The time-course evolution of viability and competence for proliferation of woody plant protoplasts following cold-storage. VII Int Protoplast Symp. (Uppsala, Sweden). Physiol Plant 82:A16.Google Scholar
  10. Ochatt SJ, Power JB (1988a) Rhizogenesis in callus from Conference pear (Pyrus communis L.) protoplasts. Plant Cell Tissue Organ Culture 13:159–164.CrossRefGoogle Scholar
  11. Ochatt SJ, Power JB (1988b) Plant regeneration from mesophyll protoplasts of Williams’ Bon Chretien (syn. Bartlett) pear (Pyrus communis L.). Plant Cell Rep 7:587–589.CrossRefGoogle Scholar
  12. Ochatt SJ, Power JB (1992) Fundamental aspects of plant regeneration from cultured cells and protoplasts. In: Moo-Young M, Warren GS, Fowler MW (eds) Comprehensive biotechnology Suppl 2. Pergamon Press, New York: 99–127.Google Scholar
  13. Ochatt SJ, Patat-Ochatt EM, Rech EL, Davey MR, Power JB (1989) Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (Pyrus communis var. pyraster L.) and Colt cherry (Prunus avium x pseudocerasus). Theor Appl Genet 78:35–41.CrossRefGoogle Scholar
  14. Ochatt SJ, Davey MR, Power JB (1990) Tissue culture and top-fruit tree species. In: Walker JM, Pollard J (eds) Methods in molecular biology vol 6: Plant cell culture. The Humana Press, Clifton, New Jersey pp 193–207.Google Scholar
  15. Ochatt SJ, Chevreau E, Gallet M (1992) Organogenesis from Passe Crassane and Old Home pear (Pyrys communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants. Theor Appl Genet 83:1013–1018.CrossRefGoogle Scholar
  16. Patat-Ochatt EM (1994) Plant regeneration from protoplasts of apple (Malus x domestica Borkh.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 29: Plant protoplasts and genetic engineering. Springer Berlin Heidelberg.Google Scholar
  17. Power JB, Davey MR, McLellan MS, Wilson D (1989) Laboratory manual: plant tissue culture. The University of Nottingham.Google Scholar
  18. Rech EL, Ochatt SJ, Chand PK, Power JB, Davey MR (1987) Electro-enhancement of division of plant protoplast-derived cells. Protoplasma 141:169–176.CrossRefGoogle Scholar
  19. Rehder A (1967) Manual of cultivated trees and shrubs, 2nd edn. McMillan, New York.Google Scholar
  20. Revilla MA, Ochatt SJ, Doughty D, Power JB (1987) A general strategy for the isolation of leaf mesophyll protoplasts from deciduous fruit and nut tree species. Plant Sci 50:133–137.CrossRefGoogle Scholar
  21. ptVan der Zwet T, Keil HL (1979) Fire blight: a bacterial disease of rosaceous plants. USDA Agric Handbook 510:200 pp.Google Scholar
  22. Vavrà M, Orel V (1971) Hybridization of pear varieties by Gregor Mendel. Euphytica 20:60–67.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • S. J. Ochatt
    • 1
  1. 1.I.N.R.A., Station d’Amélioration des Espèces Fruitières et OrnementalesCentre d’AngersBeaucouzé CédexFrance

Personalised recommendations