A General Procedure for the Purification of Fimbrial Lectins from Escherichia Coli
The presence of lectins on the surface of E. coli has been known since Duguid et al. (1955) described in the mid-1950’s the hemagglutinating properties of some E. coli strains (reviewed by Duguid and Old 1980). Especially during the past years, much effort has been devoted to the purification and characterization of surface lectins from several bacterial species (Sharon 1987; Jann and Jann 1990; Bertels et al. 1991; Gilboa-Garber and Avichezer 1993). Without doubt, the E. coli fimbrial lectins are among the most thoroughly investigated. This is not surprising, since although E. coli are advantageous commensals of the large intestine of mammals and birds, some strains are able to colonize other niches as well, and are the causative agents of both intestinal and extra-intestinal diseases in man and his domestic animals. It is now generally recognized that the attachment of bacteria to the host’s mucosae is an initial step in pathogenesis and that attachment or adhesion is mediated by bacterial surface lectins which can be of either fimbrial or nonfimbrial nature. Consequently, inhibition of bacterial adhesion is the way of choice to prevent infection (Beachy 1981).
KeywordsSugar Sucrose Filtration Albumin Urea
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- Bertels A, De Greve H, Lintermans P (1991) Function and genetics of fimbrial and nonfimbrial lectins from Escherichia coli. In: Kilpatrick DC, Van Driessche E, Bøg-Hansen TC (eds) Lectin reviews, vol 1. Sigma Chem. Comp., St. Louis MO, USA, pp. 53–67Google Scholar
- DeGraaf FK, Klemm P, Gaastra W (1980) Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99. Infec Immun 33: 877–883Google Scholar
- Duguid JP, Old DC (1980) Adhesive properties of Enterobacteriaceae. In: Beachy EH (ed) Bacterial adherence, Chapman and Hall. London, Methuen, New York, pp. 184–217Google Scholar
- Gilboa-Garber N, Avichezer D (1993) Effects of Pseudomonas aeruginosa PA-I and PA-II lectins on tumor cells. In: Gabius HJ, Gabius S (eds) Lectins and Glycobiology, Springer-Verlag, pp. 380–395Google Scholar
- Jann K, Jann B (eds) (1990) Current topics in microbiology and immunology, vol 151. Springer Verlag, Berlin-Heidelberg.Google Scholar
- Korhonen T, Nurmiaho EL, Ranta H, Svanborg Edén C (1980) New method for isolation of immunologically pure pili from Escherichia coli. Infec Immun 27: 569–575Google Scholar