A General Procedure for the Purification of Fimbrial Lectins from Escherichia Coli
The presence of lectins on the surface of E. coli has been known since Duguid et al. (1955) described in the mid-1950’s the hemagglutinating properties of some E. coli strains (reviewed by Duguid and Old 1980). Especially during the past years, much effort has been devoted to the purification and characterization of surface lectins from several bacterial species (Sharon 1987; Jann and Jann 1990; Bertels et al. 1991; Gilboa-Garber and Avichezer 1993). Without doubt, the E. coli fimbrial lectins are among the most thoroughly investigated. This is not surprising, since although E. coli are advantageous commensals of the large intestine of mammals and birds, some strains are able to colonize other niches as well, and are the causative agents of both intestinal and extra-intestinal diseases in man and his domestic animals. It is now generally recognized that the attachment of bacteria to the host’s mucosae is an initial step in pathogenesis and that attachment or adhesion is mediated by bacterial surface lectins which can be of either fimbrial or nonfimbrial nature. Consequently, inhibition of bacterial adhesion is the way of choice to prevent infection (Beachy 1981).
KeywordsAmmonium Sulfate Purification Procedure Agglutination Assay Major Subunit Serratia Species
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