Advertisement

In Situ Microdensitometry of Neoglycoprotein Staining: Detection of Tissue-Site Specific Patterns of Endogenous Lectin Expression

  • F. Vidal-Vanaclocha
  • D. Glaves
Part of the Springer Laboratory book series (SLM)

Abstract

In this chapter we introduce a microdensitometric and image analysis procedure for the in situ quantification of endogenous lectin expression by normal and altered cells in different tissues and even at specific sites within a single tissue. The large family of sugar-binding molecules can be detected by biotinylated neoglycoproteins (see Danguy an Gabius, Chap. 25, and Sinowatz et al, Chap. 27, this vol.), using a peroxidase reaction associated to the avidin-biotin interaction. The chromophore concentration precipitated at defined tissue sites and components is proportional to the number of sugar binding sites, and quantification can be done by microdensitometry, i.e., by registfating the optical density (light intensity in negative images) at specific tissue areas (pixels) of digitalized images.

Keywords

Lewis Lung Carcinoma Black Pixel Peroxidase Reaction Intensity Interval Image Analysis Procedure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gabius H-J, Bodanowitz S, Schauer A (1988) Endogenous sugar-binding proteins in human breast tissue and benign and malignant breast lesions. Cancer 61: 1125–1131PubMedCrossRefGoogle Scholar
  2. Glaves D, Gabius H-J, Weiss L (1989) Site-associated expression of endogenous tumor lectins. Int J Cancer 44: 506–511PubMedCrossRefGoogle Scholar
  3. Vidal-Vanaclocha F, Barberá-Guillem E, Weiss L, Glaves D, Gabius HJ (1990) Quantitation of endogenous lectin expression in 3LL tumors, growing subcutaneously and in the kidneys of mice. Int J Cancer 46: 908–912PubMedCrossRefGoogle Scholar
  4. Vidal-Vanaclocha F, Glaves D, Barberá-Guillem E, Weiss L (1991) Quantitative microscopy of mouse colon 26 cells growing in different metastatic sites. Br J Cancer 63: 748–752PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • F. Vidal-Vanaclocha
  • D. Glaves

There are no affiliations available

Personalised recommendations