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Bodenbiologische Arbeitsmethoden pp 224–254Cite as

Intrazellulärer Stoffwechsel

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Zusammenfassung

Die Adenin-Nukleotide AMP (Adenosin-5′-Monophosphate ADP (Adenose-5′-Diphosphat) und insbesondere das ATP (Adenosin-5′-Triphosphat) sind universelle Lager- und Transportformen zur Kopplung energieliefernder und energieverbrauchender Reaktionen in biologischen Prozessen. Die Oxidationen reduzierter Verbindungen in der Atmungskette bzw. in der Substratkettenphosphorylierung liefern die Energie für die Phosphorylierung von AMP bzw. ADP zu ATP. Das Verhältnis dieser drei Adenylate zueinander ist ein Maßstab für den physiologischen und metabolischen Status von Zellen.

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Literatur

  • Atkinson DE, Walton GM (1967) Adenosine triphosphate conservation in metabolic regulation. Rat liver cleavage enzyme. J Biol Chem 242:3239–3241

    PubMed  CAS  Google Scholar 

  • Ausmus BS (1973) The use of ATP assay in terrestrial decomposition studies. Bull Ecol Res Comm 17:223–234

    CAS  Google Scholar 

  • Bai QY, Zelles L, Scheunert I, Korte F (1988) A simple effective procedure for the determination of adenosine triphosphate in soils. Chemosphere 17:2461–2470

    Article  CAS  Google Scholar 

  • Bai QY, Zelles L, Scheunert I, Korte F (1989) Determination of adenine nucleotides in soil by ion-paired reverse-phase high-performance liquid chromatography. J Microbiol Methods 9:345–351

    Article  CAS  Google Scholar 

  • Brookes PC, Newcombe AD, Jenkinson DS (1987) Adenylate energy charge measurements in soil. Soil Biol Biochem 19:211–217.

    Article  CAS  Google Scholar 

  • Brookes PC, Tate KR, Jenkinson DS (1983) The adenylate energy charge of the soil microbial biomass. Soil Biol Biochem 15:9–16

    Article  CAS  Google Scholar 

  • Conklin AR, MacGregor AN (1972) Soil adenosine triphosphate: Extraction recovery and half-life. Bull Environ Contam Toxicol 7:296–300

    Article  PubMed  CAS  Google Scholar 

  • Davis WM, White DC (1980) Fluorometric determination of adenosinenucleotide derivatives as measure of the microfouling, detrital and sedimentary microbial biomass and physiological status. Appl Env Microbiol 40:539–548

    CAS  Google Scholar 

  • Eiland F (1979) An improved method for determination of adenosin triphosphate (ATP) in soil. Soil Biol Biochem 11:31–35

    Article  CAS  Google Scholar 

  • Fairbanks BC, Woods LE, Bryant RJ, Elliott ET, Cole CV, Coleman DC (1984) Limitations of ATP estimates of microbial biomass. Soil Biol Biochem 16:549–558

    Article  CAS  Google Scholar 

  • Holm-Hansen O (1973) The use of ATP determinations in ecological studies. Bull Ecol Res Comm (Stockholm) 17:215–222

    CAS  Google Scholar 

  • Holm-Hansen O, Booth CR (1966) The measurement of adenosine triphosphate in the ocean and its ecological significance. Limnol Oceanogr 11:510–519

    Article  CAS  Google Scholar 

  • Jenkinson DS (1988) Determination of microbial biomass, carbon and nitrogen in soils. In: Wilson JR (eds) Advances in Nitrogen Cycling in agricultural ecosysstems 1. Agricultural Land. Soils. Nitrogen Cycle. CAB International, Wallingford UK, p 368

    Google Scholar 

  • Jenkinson DS, Oades JM (1979) A method for measuring adenosine triphosphate in soil. Soil Biol Biochem 11:193–199

    Article  CAS  Google Scholar 

  • Karl DM (1980) Cellular nucleotide measurements and applications in microbial ecology. Microbiol Reviews 44:739–796

    CAS  Google Scholar 

  • Lee CC, Harris RF, Williams JDH, Armstrong DE, Syers JK (1971) Adenosine triphosphate in lake sediments: I Determination. Soil Sci Soc Am Proc 35:82–86

    Article  CAS  Google Scholar 

  • Maire N (1984) Extraction de 1′ adenosine triphosphate dans les sols: une nouvelle methode de calcul des pertes en ATP. Soil Biol Biochem 16:361–366

    Article  CAS  Google Scholar 

  • Martens R (1985) Estimation of the adenylate energy charge in unamended and amended agricultural soils. Soil Biol Biochem 17:765–772

    Article  CAS  Google Scholar 

  • Paul EA, Johnson RL (1977) Microscopic counting and adenosine 5′-triphosphate measurement in determining microbial growth in soils. Appl Env Microbiol 34:263–269

    CAS  Google Scholar 

  • Rosacker LL, Kieft TL (1990) Biomass and Adenylate Energy Charge of a grassland soil during drying. Soil Biol Biochem 22:1121–1127

    Article  Google Scholar 

  • Sparling GP, Eiland F (1983) A comparison of methods for measuring ATP and microbial biomass in soils. Soil Biol Biochem 15:227–229

    Article  CAS  Google Scholar 

  • Vaden VR, Webster JJ, Hampton GJ, Hall MS, Leach FR (1987) Comparison of methods for extraction of ATP from soil. J Microbiol Methods 7:211–217

    Article  CAS  Google Scholar 

  • Verstraete W, Van De Werf H, Kucnerowicz F, Ilaiwi M, Verstraeten LMJ, Vlassak K (1983) Specific measurement of soil microbial ATP. Soil Biol Biochem 15:391–396

    Article  CAS  Google Scholar 

  • Verstraeten LMJ, De Coninck K, Vlassak K, Verstraete W, Van De Werf H,Iliawi M (1983) ATP content of soils estimated by two contrasting estimation methods. Soil Biol Biochem 15:397–402

    Article  CAS  Google Scholar 

  • Webster JJ, Hampton GJ, Leach FR (1984) ATP in soil: a new extractant and extraction procedure. Soil Biol Biochem 16:335–342

    Article  CAS  Google Scholar 

  • Zelles L, Scheunert I, Korte F (1985) ATP-measurements in soil: A combination between the TCA and NRB extraction methods. Chemosphere 14:139–148

    Article  CAS  Google Scholar 

  • Bai QY, Zelles L, Scheunen I, Korte F (1989) Determination of adenine nucleotides in soil by ion-paired reverse-phase high-performance liquid chromatography. J Microbiol Methods 9:345–351

    Article  CAS  Google Scholar 

Literatur

  • Jenkinson DS, Davidson SA, Powlson DS (1979) Adenosine triphosphate and microbial biomass in soil. Soil Biol Biochem 11:521–527

    Article  CAS  Google Scholar 

  • Jenkinson DS, Oades JM (1979) A method for measuring adenosine triphosphate in soil. Soil Biol Biochem 11:193–199

    Article  CAS  Google Scholar 

  • Margesin R (1986) Die Bestimmung von Adenosintriphosphat in Bodenmaterialien. Diplomarbeit Universität Innsbruck

    Google Scholar 

  • Sparling GP, Eiland F (1983) A comparison of methods for measuring ATP and microbial biomass in soils. Soil Biol Biochem 15:227–229

    Article  CAS  Google Scholar 

  • Thore A, Rawlins T (1980) Chemiluminescent analysis in the life sciences. LKB-Wallac, Turku Finland

    Google Scholar 

Literatur

  • Eiland F (1979) An improved method for determination of adenosin triphosphate (ATP) in soil. Soil Biol Biochem 11:31–35

    Article  CAS  Google Scholar 

  • Lee CC, Harris RF, Williams JDH, Armstrong DE, Syers JK (1971) Adenosine triphosphate in lake sediments: I. Determination. Soil Sci Soc Am Proc 35:82–86

    Article  CAS  Google Scholar 

  • Maire N (1982) Methode de mesure de 1’adenosine triphosphate (ATP) dans les sols. Bulletin BGS 6:88–94

    Google Scholar 

  • Maire N (1984) Extraction de l’adenosine triphosphate dans les sols: une nouvelle methode de calcul des pertes en ATP. Soil Biol Biochem 16:361–366

    Article  CAS  Google Scholar 

  • Margesin R (1986) Die Bestimmung von Adenosintriphosphat in Bodenmaterialien. Diplomarbeit Universität Innsbruck

    Google Scholar 

  • Thore A (1979) Technical aspects of the bioluminescent firefly luciferase assay of ATP. Sci Tools 26:30–34

    CAS  Google Scholar 

Literatur

  • Baroccio A (1958) Catalase activity of soil as a biopedological index of fertility. Agrochimica 2:243–257

    Google Scholar 

  • Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bodenkd 130:68–81

    Article  CAS  Google Scholar 

  • Benefield CB, Howard PJA, Howard DM (1977) The estimation of dehydrogenase activity in soil. Soil Biol Biochem 9:67–70

    Article  CAS  Google Scholar 

  • Casida LEJr, Klein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376

    Article  CAS  Google Scholar 

  • Curl H, Sandberg J (1961) The measurement of dehydrogenase activity in marine organisms. J Mar Res 19:123–138

    CAS  Google Scholar 

  • Glathe H, Thalmann H (1970) Über die mikrobielle Aktivität und ihre Beziehungen zu Fruchtbarkeitsmerkmalen einiger Ackerböden unter besonderer Berücksichtigung der Dehydrogenaseaktivität. 2. Mitt Zbl Bakt II 124:24–36

    CAS  Google Scholar 

  • Griffiths BS (1989) Improved extraction of iodonitrotetrazolium-formazan from soil with dimethylformamide. Soil Biol Biochem 21:179–180

    Article  CAS  Google Scholar 

  • Johnson JL, Temple KL (1964) Some variables affecting the measurement of “catalase activity” in soil. Soil Sci Soc Am Proc 28:207–209

    Article  CAS  Google Scholar 

  • Kuprevich VF, Shcherbakova TA (1956) Determination of invertase and catalase activity of soils. Vestsi Akad Navuk Belarusk SSR, Ser Biyal 2:115–116

    Google Scholar 

  • Kuprevich VF, Shcherbakova TA (1971) Soil Enzymes. US Department of Commerce, National Technical Information Service, Springfield Virginia

    Google Scholar 

  • Lenhard G (1956) Die Dehydrogenaseaktivität des Bodens als Maß für die Mikroorganismentätigkeit im Boden. Z Pflanzenernaehr Bodenkd 73:1–11

    Article  CAS  Google Scholar 

  • Skujins J (1978) History of abiontic soil enzyme research. In: Burns RG (ed) Soil Enzymes. Academic Press, London New York San Francisco, p 1

    Google Scholar 

  • Stevenson IL (1959) Dehydrogenase activity in soils. Can J Microbiol 5:229–235

    Article  PubMed  CAS  Google Scholar 

  • Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR (eds) Methods of Soil Analysis, Part 2. Am Soc Agron Inc, Soil Sci Soc Am Inc, Madison Wiscinson USA, p 903

    Google Scholar 

  • Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivität im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249–258

    CAS  Google Scholar 

  • Trevors JT (1984) Dehydrogenase activity in soil: a comparsion between the INT and TTC assay. Soil Biol Biochem 16:673–674

    Article  CAS  Google Scholar 

  • Mersi von W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11:216–220

    Article  Google Scholar 

  • Woods AF (1899) The destruction of chlorophyll by oxidizing enzymes. Zentralbl Bakteriol Parasitenkd Abt 2 5:745–754

    Google Scholar 

Literatur

  • Beck T (1971) Die Messung der Katalaseaktivität von Böden. Z Pflanzenernaehr Bodenkd 130:68–81

    Article  CAS  Google Scholar 

  • Stefanic G, Beck T, Schwemmer J, Hartmann F, Varbancin A (1984) Apparatur for measuring soil catalase activity. 5th Symp Soil Biol, JASJ Buckarest:47–50

    Google Scholar 

  • Trevors JT (1984) Rapid gaschromatographic method to measure H2O2 oxidoreductase activity in soil. Soil Biol Biochem 16:525–526

    Article  CAS  Google Scholar 

Literatur

  • Glathe H, Thalmann H (1970) Über die mikrobielle Aktivität und ihre Beziehungen zu Fruchtbarkeitsmerkmalen einiger Ackerböden unter besonderer Berücksichtigung der Dehydrogenaseaktivität. 2. Mitt Zbl Bakt II 124:24–36

    CAS  Google Scholar 

  • Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivität im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249–258

    CAS  Google Scholar 

Literatur

  • Mersi von W, Schinner F (1991) An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils 11:216–220

    Article  Google Scholar 

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Authors
  1. B. Albers
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  2. R. Rackwitz
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  3. M. Raubuch
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  4. L. Zelles
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  5. R. Margesin
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  6. F. Schinner
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  7. R. Öhlinger
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  8. W. von Mersi
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Editor information

Editors and Affiliations

  1. Institut für Mikrobiologie, Universität Innsbruck, Technikerstraße 25, A-6020, Innsbruck, Austria

    Franz Schinner

  2. Bundesanstalt für Agrarbiologie, Wieningerstraße 8, A-4025, Linz, Austria

    Richard Öhlinger

  3. Bundesanstalt für Bodenwirtschaft, Denisgasse 31-33, A-1200, Wien, Austria

    Ellen Kandeler

  4. Christian Doppler Laboratorium für Umweltbiotechnologie, Technikerstraße 25, A-6020, Innsbruck, Austria

    Rosa Margesin

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© 1993 Springer-Verlag Berlin Heidelberg

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Albers, B. et al. (1993). Intrazellulärer Stoffwechsel. In: Schinner, F., Öhlinger, R., Kandeler, E., Margesin, R. (eds) Bodenbiologische Arbeitsmethoden. Springer Labor. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77936-7_8

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  • DOI: https://doi.org/10.1007/978-3-642-77936-7_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77937-4

  • Online ISBN: 978-3-642-77936-7

  • eBook Packages: Springer Book Archive

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