Reepithelialisation of Wounds
Reepithelialisation of wounds involves a number of different processes affecting the biology of the keratinocyte. Following breaching of the epidermis the keratinocytes must first migrate across the surface of the denuded area, then mitosis must occur close to the edge to expand the population (Odland and Ross 1968; Krawczyck 1971; Clark 1985). Once keratinocytes have covered the defect they must establish a basement membrane zone, which aids keratinocyte attachment. Then stratification and differentiation must occur to normalise the newly regenerated epidermis. Subsequently the dermis is remodelled, which also involves the keratinocytes by keratinocytemesenchymal interactions mediated by cell contact, diffusible cytokines and extracellular matrix proteins. Replacement of a denuded area of skin by different forms of skin grafts, including keratinocyte sheets, will accelerate the epithelialisation process. These biological processes of keratinocytes will be discussed following an introduction to keratinocyte culture, which provides the basis for many observations applicable to wound healing.
KeywordsMigration Hydrocortisone Stratification Hull Fibril
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