Advertisement

Mechanisms of Action of Collagenase in Wound Repair

  • R. A. Hatz
  • N. C. S. von Jan
  • F.-W. Schildberg
Conference paper

Abstract

Specific collagenases possess the unique capability of degrading native collagen which is otherwise resistant to breakdown by other known tissue proteases [1]. Reports suggest that degradation products of collagen released after cleavage by collagenase may in turn control the migration and activity of important inflammatory cells such as wound macrophages and, therefore, at a very early stage in wound repair substantially influence the healing process [2–4]. Abnormal collagen deposition as a result of alterations in collagenolysis may be seen in various pathologic conditions such as hypertrophic scars and keloids [5]. Excessively high collagenase activity is found in rheumatoid arthritis, local tumor invasion, excessive bone resorption following bone injury, anastomotic insufficiency following bowel surgery and post-burn granulation tissue [6–11] (Table 1). Selective therapeutic enhancement or blockage of the collagenolytic system may therefore prove a valuable tool in treatment or prevention of these conditions.

Keywords

Wound Repair Hypertrophic Scar Native Collagen Collagenase Activity Preferential Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Harris ED Jr, Krane SM (1974) Collagenases (first of three parts). N Engl J Med 291(11): 557–563.PubMedCrossRefGoogle Scholar
  2. 2.
    Postlethwaite AE, Kang AH (1976) Collagen-and collagen peptide-induced chemotaxis of human blood monocytes. J Exp Med 143:1299–1307.PubMedCrossRefGoogle Scholar
  3. 3.
    Wize J, Woitecka-Lukasik E, Maslinski S (1986) Collagen-derived peptides release mast cell histamine. Agents Actions 18:262–265.PubMedCrossRefGoogle Scholar
  4. 4.
    Postlethwaite AE, Seyer JM, Kang AH (1978) Chemotactic attraction of human fibroblasts to type I, II and III collagens and collagen derived peptides. Proc Natl Acad Sci USA 75:871–875.PubMedCrossRefGoogle Scholar
  5. 5.
    Craig P (1973) Collagenase activity in cutaneous scars. Hand 5(3):239–246.PubMedCrossRefGoogle Scholar
  6. 6.
    Dresden MH, Heilmann SA, Schmidt JD (1972) Collagenolytic enzymes in human neoplasms. Cancer Res 32:993–996.PubMedGoogle Scholar
  7. 7.
    Liotta LA, Tyggvason K, Gorbisa S, Hart J, Foltz CM, Shafie S (1980) Metastatic potential correlates wigh enzymatic degradation of basement membrane collagen. Nature 284:67–68.PubMedCrossRefGoogle Scholar
  8. 8.
    Young HC, Wheeler MH (1983) Collagenase inhibition in the healing colon. J R Soc Med 76(1):32–36.PubMedGoogle Scholar
  9. 9.
    Kishi K, Hashimoto Y, Aoyama H, Izawa Y, Hayakawa T (1984) Direct extraction of collagenase from human post-burn wound tissues. Biomed Res 5:149–156.Google Scholar
  10. 10.
    Grillo HC, Gross J (1967) Collagenolytic activity during mammalian wound repair. Dev Biol 15:300–317.PubMedCrossRefGoogle Scholar
  11. 11.
    Black CT, Hennessey PJ, Ford EG, Andrassy RJ (1989) Protein glycosylation and collagen metabolism in normal and diabetic rats. J Surg Res 47:200–202.PubMedCrossRefGoogle Scholar
  12. 12.
    Gross J, Nagai Y (1965) Specific degradation of the collagen molecule by tadpole collagenolytic enzyme. Proc Natl Acad Sci USA 54:1197–1204.PubMedCrossRefGoogle Scholar
  13. 13.
    Gross J, Highberger JH, Johnson-Wint B, Biswas C (1980) In: Wooley DE, Evanson JM (eds) Collagenase in normal and pathological tissues. Wiley, Sussex, p 11.Google Scholar
  14. 14.
    Birkedal-Hansen B, Moore WGI, Taylor RE, Bhown AS, Birkedal-Hansen H (1988) Monoclonal antibodies to human fibroblast procollagenase. Inhibition of enzymatic activitiy, affinity purification of the enzyme and evidence for clustering of epitopes in the NH2-terminal end of the activated enzyme. Biochemistry 27:6751–6758.PubMedCrossRefGoogle Scholar
  15. 15.
    Mallya SK, Mookhtiar KA, Gao Y, Brew K, Dioszegi M, Birkedal-Hansen H, Van Wart HE (1990) Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase. Biochemistry 29:10628–10634.PubMedCrossRefGoogle Scholar
  16. 16.
    Adelmann-Grill BC (1982) Säugetierkollagenasen: physiologische und pathophsiologische Aspekte. Arzneimittelforschung 32(11) 10a: 1357–1362.PubMedGoogle Scholar
  17. 17.
    Eisen AZ (1969) Human skin collagenase: localization and distribution in normal human skin. J Invest Dermatol 52:442–448.PubMedGoogle Scholar
  18. 18.
    Donoff B, McLennon JE, Grillo HC (1971) Preparation and properties of collagenases from epithelium and mesenchyne of healing mammalian wounds. Biochim Biophys Acta 227:639.PubMedGoogle Scholar
  19. 19.
    Dayer J-M, Goldring SR, Robinson DR, Krane SM (1980) In: Wooley DE, Evanson JM (eds) Collagenase in normal and pathological connective tissues. Wiley, Sussex.Google Scholar
  20. 20.
    Fullmer HM, Taylor RE, Guthrie RM (1972) Human gingival collagenase: purification, molecular weight and inhibitor studies. J Dent Res 51:349–355.PubMedCrossRefGoogle Scholar
  21. 21.
    Tschesche H, Macartney HW (1981) A new principle of regulation of enzymatic activity: activation and regulation of human PMNL collagenase via disulfide-thiol exchange as catalysed by the glutathion cycle in a peroxidase coupled reaction to glucose metabolism. Eur J Biochem 120:183.PubMedCrossRefGoogle Scholar
  22. 22.
    Ohlson K, Ollson I, Spitznagel JK (1977) Localisation of chymotrypsin-like cationic protein, collagenase and elastase in azurophilic granules of human PMNLs. Hoppe Seylers Z Physiol Chem 358:361.CrossRefGoogle Scholar
  23. 23.
    Werb Z, Gordon S (1975) Secretion of a specific collagenase by stimulated macrophages. J Exp Med 142:346.PubMedCrossRefGoogle Scholar
  24. 24.
    Dayer J-M, Breard J, Chess L, Krane SM (1979) Participation of monocyte-macrophages and lymphocytes in the production of a factor that stimulates collagenase and protaglandin release by rheumathoid synovial cells. J Clin Invest 64:1386.PubMedCrossRefGoogle Scholar
  25. 25.
    Postlethwaite AE, Lachman LB, Mainardi CL, Kang AH (1983) Interleukin 1 stimulation of collagenase production by cultured fibroblasts. J Exp Med 157:801–806.PubMedCrossRefGoogle Scholar
  26. 26.
    Bauer EA, Cooper TW, Huang JS, Altman J, Deuel TF (1985) Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor. Proc Natl Acad Sci USA 82:4132–4136.PubMedCrossRefGoogle Scholar
  27. 27.
    Salo T, Lyons LG, Rahemtulla F, Birkedal-Hansen H, Larjava H (1991) Transforming growth facor beta 1 upregulates types IV collagenase expression in cultured human keratinocytes. J Biol Chem 266:11436–11441.PubMedGoogle Scholar
  28. 28.
    Buckley-Sturrock A, Woodward SC, Senior RM, Griffin GL, Klagsbrun M (1989) Differential stimulation of collagenase and chemotaxic activity in fibroblasts derived from rat wound repair tissue and human skin by growth factors. J Cell Physiol 138:70–78.PubMedCrossRefGoogle Scholar
  29. 29.
    Wahl LM, Olsen CE, Sandberg AL, Mergenhagen SE (1977) Prostaglandin regulation of macrophage collagenase production. Proc Natl Acad Sci USA 74:4955.PubMedCrossRefGoogle Scholar
  30. 30.
    Brinckerhoff CE, McMillan RM, Dayer J-M, Harris ED (1980) Inhibition by retinoic acid of collagenase production in rheumatoid synovial cells. N Engl J Med 303:432.PubMedCrossRefGoogle Scholar
  31. 31.
    Brinckerhoff CE, Harris ED (1981) Modulation by retinoic acid and corticosteroids of collagenase production by rabbit synovial fibroblasts treated with phorbol myrivate acetate or polyethylene glycol. Biochim Biophys Acta 677:424.PubMedCrossRefGoogle Scholar
  32. 32.
    Passwell JH, Dayer JM, Gass K, Edelson PJ (1980) Regulation by Fc fragments of the secretion of collagenase, PGE2 and lysozyme by mouse peritoneal macrophages. J Immunol 125:910.PubMedGoogle Scholar
  33. 33.
    Welgus HG, Jeffrey JJ, Eisen AZ (1981) The collagen substrate specificity of human skin fibroblast collagenase. J Biol Chem 256:9511.PubMedGoogle Scholar
  34. 34.
    Horwitz AL, Hance AJ, Crystal RG (1977) Granulocyte collagenase: selective digestion of type I relative to type III collagen. Proc Natl Acad Sci USA 74:897.PubMedCrossRefGoogle Scholar
  35. 35.
    Werb Z, Burleigh MC, Barret AJ, Starkey PM (1974) The interaction of alpha2-macroglobulin with proteases, binding and inhibition of mammalian collagenases and other metal proteinases. Biochem J 139:359.PubMedGoogle Scholar
  36. 36.
    Seifter S, Harper E (1970) Collagenases. Methods Enzymol 19:613–635.CrossRefGoogle Scholar
  37. 37.
    Reil B (1986) Function of animal and bacterial collagenase. Am Biol Clin 44:168–175.Google Scholar
  38. 38.
    Mandl J (1982) Bacterial collagenases and their clinical applications. Arzneimittelforschung 32(11) 10a:1381–1384.PubMedGoogle Scholar
  39. 39.
    Lee LK, Ambrus JL (1975) Collagenase therapy for decubitus ulcers. Geriatrics 30:91–98.PubMedGoogle Scholar
  40. 40.
    Varma AO, Bugatch E, Gemram FM (1973) Debridement of dermal ulcers with collagenase. Surg Gynecol Obstet 136:281–282.PubMedGoogle Scholar
  41. 41.
    Paul E (1990) Wundheilung unter IruxolR. Fortschr Med 35:679–681.Google Scholar
  42. 42.
    Blum G (1973) Therapeutische Erfahrungen mit IruxolR bei Ulcera cruris, Dekubitus und Verbrennungen. Schweiz Rundschau Med Prax 62:820–826.Google Scholar
  43. 43.
    Purder K (1973) Erfahrungsbericht über die Anwendung von IruxolR-Salbe bei Verbrennungen. Z Allgemeinmed 49:856–858.PubMedGoogle Scholar
  44. 44.
    Cohen IK, Diegelmann PF, Keiser HR Collagen metabolism in keloid and hypertrophic scar. In: Longacre JJ (ed) The ultrastructure of collagen. Thomas, Springfield, pp 199-212.Google Scholar
  45. 45.
    Friedman K, Pollack SV, Manning T, Pinell SR (1986) Degradation of porcine dermal connective tissue by collagenase and hyaluronidase. Br J Dermatol 115:403–408.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • R. A. Hatz
    • 1
  • N. C. S. von Jan
    • 1
  • F.-W. Schildberg
    • 1
  1. 1.Department of Surgery, Klinikum GroßhadernLudwig-Maximilians-UniversityMünchenGermany

Personalised recommendations