Three-Dimensional Reconstruction of Terminal Blood Spaces in the Proximal Tibia Metaphysis of the Growing Rat — A Model to Study Normal Angiogenesis
Since Albrecht von Haller’s (1763) suggestion that the vascular system is responsible for osteogenesis, anatomists have tried to elucidate the vascular pattern in the metaphysis of the growing bone. Our primary interest was the development of capillary sprouts and their conversion into new capillary loops. Since the tibia grows unidirectionally, one would expect that its capillaries follow a similar pattern. In order to study all vessel-like structures, including sprouts without an active hemoperfusion, we used serial sectioning instead of corrosion cast techniques. To understand the growth and hemoperfusional pattern it is important to visualize both cartilaginous cavities and intertrabecular spaces (ITS), and the capillaries and capillary sprouts.
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