Advertisement

Neutralization of HIV-1: A Summary

  • Nigel J. Dimmock
Chapter
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 183)

Abstract

The pressing need to devise a vaccine against HIV-1 fuels an intensive and ongoing study of its neutralization. The envelope protein has been sequenced and a structure computed (Ratner et al. 1985; Modrow et al. 1987; Leonard et al. 1990) but understanding of functional relationships awaits its crystallization and determination of its atomic structure (see reviews by McKeating and Willey 1989; Putney and McKeating 1990). The envelope protein is heavily glycosylated (about 50% of its mass is carbohydrate) and is post-translationally cleaved into the anchor, gp41, and a non-covalently linked distal segment gp120. Two or four of these units form the mature spike protein (Thomas et al. 1991) and there are 70–80 spikes per virion (Gelderblom et al. 1987). By comparing amino acid sequences of seven different strains, Modrow et al. (1987) identified five variable (V) and six constant (C) regions interspersed throughout gp120 and gp41. These and some information relevant to neutralization are shown in Figs. 2 and 10. The use of synthetic peptides to elicit or identify reactive antibody has located 12 possible antigenic sites, ten of which are neutralizing, although some of these are probably part of the same discontinuous site (Table 14; Fig. 10). Nonneutralization sites are also known on gp120 and gp41 (e.g. Banapour et al. 1987; Lasky et al. 1987; Linsley et al. 1988; Kinney-Thomas et al. 1988; Ardman et al. 1990; Larcher et al. 1990; Teeuwsen et al. 1990; Robinson et al. 1991; Xu et al. 1991) and include epitopes on the neutralization immunodominant V3 loop (Langedijk et al. 1991 ; Laman et al. 1992). Attention in regard to neutralization has concentrated on the V3 loop (Fig. 2b) which is concerned with the entry process and, in particular, the fusion of viral and cellular membranes.

Keywords

Envelope Protein Attachment Site Antigenic Site Compare Amino Acid Sequence Neutralization Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Nigel J. Dimmock
    • 1
  1. 1.Department of Biological SciencesUniversity of WarwickCoventryUK

Personalised recommendations