Skip to main content
SpringerLink
Log in
Book cover

Immuntherapie in der Uroonkologie pp 41–45Cite as

Multizelluläre Sphäroide als Testsubstanz für neue Therapieformen

  • Conference paper

  • 24 Accesses

Zusammenfassung

In-vitro-Kultmen und experimentelle Tiermodelle sind wichtige Hilfsysteme, um fundamentale Tumor- und Tumorzelleigenschaften zu verstehen und Therapiemodalitäten für menschliche Tumoren zu etabüeren. Im folgenden wird ein dreidimensionales In-vitro-System, das Multizelluläre Sphäroid (multi-cellular spheroid = MCS), vorgestellt, welches in der Tumorforschung zunehmende Verbreitung findet (Acker et al. 1984; Bjerkvig 1990; Knüchel u. Sutherland 1990; Sutherland 1987). Wichtige Aspekte der Immuntherapie wie Antigenverteilung, Antikörperpenetration und auch Migrations- und Toxizitätseigenschaften immunkompetenter Zellen können mittels MCS einer systematischen Untersuchung zugänglich gemacht werden.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   69.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  • Acker H, Carlsson J, Durand R, Sutherland RM (eds) (1984) Spheroids in Cancer research: Methods and perspectives. Springer, Berlin Heidelberg New York (Recent results in Cancer Research 95)

    Google Scholar 

  • Acker H, Pietruschka F, Deutscher J (1990) Endothelial cell mitogen released from HT-29 tumor cells grown in monolayer or multicellular spheroid culture. Br J Cancer 62:376–380

    Article  PubMed  CAS  Google Scholar 

  • Bjerkvig R (1990) Spheroid culture in cancer research. CRC Press, Florida

    Google Scholar 

  • Bjerkvig R, Hostmark J, Petersen P-H, Laerum OD (1990) Tumor spheroids from biopsy specimens. In: Bjerkvig R (ed) Spheroid culture in cancer research. CRC Press, Florida, pp 41–58

    Google Scholar 

  • Carlsson J (1990) Tumor spheroids in studies of immunotherapy. In: Bjerkvig R (ed) Spheroid culture in cancer research. CRC Press, Florida, pp 277–306

    Google Scholar 

  • DeBruyne GK, Bracke ME, Plessers L, Mareel MM (1988) Invasiveness in vitro of mixed aggregates composed of two human mammary cell Unes MCF-7 and HBL-100. Invasion Metastasis 8: 253–265

    CAS  Google Scholar 

  • Jaaskelainen J, Lethonen E, Heikilla P, Kalliomaki P, Timonen T (1990) Damage to multicellular human H-2 glioma spheroids incubated with LAK cells: An ultrastructural study. J Natl Cancer Inst 82: 497–501

    Article  PubMed  CAS  Google Scholar 

  • Knuechel R, Sutherland RM (1990) Review: Recent Developments in research with human tumor spheroids. Cancer J 3: 234–243

    Google Scholar 

  • Knuechel R, Feichtinger J, Recktenwald A et al. (1988) Interactions between bladder tumor cells as spheroids from the cell line J82 and human endothelial cells in vitro. J Urol 139: 640–645.

    Google Scholar 

  • Knuechel R, Keng PC, Hofstaedter F, Langmuir VK, Sutherland RM, Penney DP (1990) Differentiation patterns in two- and threedimensional culture systems of human squamous carcinoma cell lines. Am J Pathol 137: 725–736

    PubMed  CAS  Google Scholar 

  • Mansbridge J, Knuechel R, Knapp AM, Sutherland RM (1992) Importance of Tyrosine Phosphatases in the effects of cell-cell contact and microenvironments on EGF-stimulated tyrosine phosphorylation. J Cell Physiol 151: 433–442

    Article  PubMed  CAS  Google Scholar 

  • Mueller-Klieser W (1987) Multicellular spheroids: A review on cellular aggregates in cancer research. J Cancer Res Clin Oncol 113: 101–122

    Article  PubMed  CAS  Google Scholar 

  • Offner FA, Wirtz HC, Schiefer J et al. (1990) Melanom-Endothelzell-Interaktion: Sauerstoffradikale als Ursache der Schädigung humaner Umbilical-Venen-Endothelzellen. Verh Dtsch Ges Pathol 74: 587

    Google Scholar 

  • Sutherland RM (1986) Importance of critical metabolites and cellular interactions in the biology of microregions of tumors. Cancer 58: 1668–1680

    Article  PubMed  CAS  Google Scholar 

  • Sutherland RM (1987) Cell and environment interactions in tumor microregions: The multicell spheroid model. Sience 240: 177–184

    Article  Google Scholar 

  • Sutherland RM, Sordat B, Bamat J, Gabber H, Bourrat B, MuellerKlieser W (1986) Oxygenation and differentiation of multicellular spheroids of human colon carcinoma. Cancer Res 46: 5320–5328

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. R. Knüchel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Hofstaedter
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Urologische Klinik und Poliklinik, Universitätsklinikum Essen, Hufelandstr. 55, W-4300, Essen 1, Deutschland

    Herbert Rübben  & Mark Goepel  & 

  2. Urologische Universitätsklinik, Moorenstr. 5, W-4000, Düsseldorf 1, Deutschland

    Bernd J. Schmitz-Dräger

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Knüchel, R., Hofstaedter, F. (1993). Multizelluläre Sphäroide als Testsubstanz für neue Therapieformen. In: Rübben, H., Goepel, M., Schmitz-Dräger, B.J. (eds) Immuntherapie in der Uroonkologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77830-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77830-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77831-5

  • Online ISBN: 978-3-642-77830-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation