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In Vitro Cultivation of Human Dermal Microvascular Endothelial Cells as a Model for the Dermatopharmacology

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Cell and Tissue Culture Models in Dermatological Research

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Abstract

The cultivation of endothelial cells began with the experiments of Lewis [35], who described the first successful outgrowth of vascular epithelial cells from explant cultures in 1922. In the early 1970s, Maruyama [38] described a technique for isolation and cultivation of endothelial cells from the large vessels. He found that the large-vessel epithelial cells could be enzymatically released from the umbilical vein by exposure to trypsin and that these cells could be cultured on coverslips coated with collagen. In the same year, 1973, Jaffe et al. [31] described a similar technique for separation and in vitro cultivation of human umbilical vein endotheliocytes. Their protocol served then as a basis for the development of further modifications of isolation and in vitro cultivation of endothelial cells from different animal and human organs.

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Authors
  1. Zb. Ruszczak
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  2. R. Stadler
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Editor information

Editors and Affiliations

  1. Zentrum der Dermatologie und Venerologie Abt. 1, Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, 6000, Frankfurt/Main 70, Germany

    August Bernd

  2. AK Kinetische Zellforschung, Institut für Zoologie, Johann Wolfgang Goethe-Universität, Senckenberganlage 27, 6900, Frankfurt/Main 70, Germany

    Jürgen Bereiter-Hahn

  3. Basotherm GmbH, Dermatologische Entwicklung, Eichendorffweg 5, 7950, Biberach a. d. Riss, Germany

    Frank Hevert

  4. Zentrum der Dematologie und Venerologie, Abt. 1, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, 6000, Frankfurt/Main 70, Germany

    H. Holzmann

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© 1993 Springer-Verlag Berlin Heidelberg

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Ruszczak, Z., Stadler, R. (1993). In Vitro Cultivation of Human Dermal Microvascular Endothelial Cells as a Model for the Dermatopharmacology. In: Bernd, A., Bereiter-Hahn, J., Hevert, F., Holzmann, H. (eds) Cell and Tissue Culture Models in Dermatological Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77817-9_34

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  • DOI: https://doi.org/10.1007/978-3-642-77817-9_34

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77819-3

  • Online ISBN: 978-3-642-77817-9

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