Abstract
In the present study we cultured human dermal fibroblasts within type-I collagen gels and in a monolayer. We found that the collagen synthesis of fibroblasts cultured in a gel was significantly reduced when compared with conventional monolayer cultures. Furthermore, we studied the regulation of collagen synthesis by the cytokines interleukin-1β (IL-1), interferon-γ-(IFN-γ),and transforming growth factor β (TGFβ) in monolayer and gel culture. While 11-1 showed no significant influence on either of the culture systems, IFNγ led to different results in a dose-dependent manner, i.e., stimulation at lower doses and inhibition at higher ones in the gel. TGFβ markedly stimulated the collagen synthesis in both monolayer and gel cultures. These data make it obvious that the specific properties of the culture system used are to be considered when studying functional parameters of fibroblasts and their regulation.
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© 1993 Springer-Verlag Berlin Heidelberg
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Herrmann, K., Kunzelmann, V., Bruns, M., Haustein, UF. (1993). Cytokine-dependent Regulation of Human Dermal Fibroblasts Cultured in a Three-dimensional Collagen-Gel. In: Bernd, A., Bereiter-Hahn, J., Hevert, F., Holzmann, H. (eds) Cell and Tissue Culture Models in Dermatological Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77817-9_25
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DOI: https://doi.org/10.1007/978-3-642-77817-9_25
Publisher Name: Springer, Berlin, Heidelberg
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