Skip to main content

Cytological Characterization of Three-dimensional, Graftable Human Keratinocyte Cultures

  • Conference paper
Cell and Tissue Culture Models in Dermatological Research
  • 115 Accesses

Abstract

Although the successful application of graftable cultured human keratinocytes depends on the cytological state, they still lack exact qualitative and quantitative characterization. Our investigations revealed that graftable keratinocyte cultures can be quantitatively characterized using a new cell-size analyzer (PPSA) and the radioactive EGF-ligation technique. With both techniques, typical changes in culture development can be proven.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barrandon Y, Green H (1985) Cell size as a determinant of the clone-forming ability of human keratinocytes. Proc Natl Acad Sci USA 82: 5390–5394

    Article  PubMed  CAS  Google Scholar 

  2. Barrandon Y, Green H (1987) Three clonal types of keratinocytes with different capacities for multiplication. Proc Natl Acad Sci USA 84: 2302–2306

    Article  PubMed  CAS  Google Scholar 

  3. Barrandon Y, Green H (1987) Cell migration is essential for sustained growth of kertinocytes colonies: the role of TGFalpha and EGF. Cell 50: 1131–1137

    Article  PubMed  CAS  Google Scholar 

  4. Boonstra J, de Laat S, Ponec M (1986) Epidermal growth factor receptor expression related to differentiation capacity in normal and transformed keratinocytes. Exp Cell Res 161: 421–433

    Article  Google Scholar 

  5. Boonstra J, Ponec M (1989) Proliferating, transformed keratinocytes cultured under low Ca2+ conditions exhibit high-affinity EGF-receptors. Cell Biol Int Rep 13: 609–617

    Article  PubMed  CAS  Google Scholar 

  6. Rheinwald JG, Green H (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6: 331–344

    Article  PubMed  CAS  Google Scholar 

  7. Rheinwald JG, Green H (1977) Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 256: 421–424

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Lang, E., Maier, K. (1993). Cytological Characterization of Three-dimensional, Graftable Human Keratinocyte Cultures. In: Bernd, A., Bereiter-Hahn, J., Hevert, F., Holzmann, H. (eds) Cell and Tissue Culture Models in Dermatological Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77817-9_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-77817-9_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77819-3

  • Online ISBN: 978-3-642-77817-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics