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Mechanical Stimulation Enhances Human Keratinocyte Differentiation in Culture: Induction of Cytokeratin 9 Synthesis

  • F. E. Görmar
  • A. Bernd
  • J. Bereiter-Hahn
  • H. Holzmann

Abstract

Epidermal cells are committed to terminal differentiation, thereby undergoing a series of morphological and biochemical changes. A specific biochemical differentiation marker of keratinocytes is the expression of the cytokeratin pair K1 and K10/11 or K1 and K9 in footpad and palm keratinocytes. Considering that in vivo, keratinocytes are subjected continuously to mechanical stress, we investigated the effect of cyclic mechanical pressure on differentiaton: HaCaT cells (spontaneously transformed human keratinocytes) were cyclically stimulated by the pressure of Teflon weights. The stimulation process lasted 1–6 days. Consequent to the pressure treatment, multilayered culture growth occurred and the pattern of cytokeratin was modified. The relative amount of suprabasal keratins was increased. After 2 days of stimulation a newly synthesized keratin (64 kD, pI 5.4) was demonstrated, and in western blots it was identified as cytokeratin K9. To exclude the possibility that cytokeratin K9 was a mere laboratory contamination, autoradiography was performed. These results, gained in vitro, demonstrate that mechanical pressure is a notable factor regarding the induction of the keratin K9 synthesis, a specific differentiation marker of palmar and plantar keratinocytes.

Keywords

Mechanical Stimulation HaCaT Cell Pyknotic Nucleus Plantar Epidermis Keratin Pattern 
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References

  1. 1.
    Bereiter-Hahn J, Anderson OR, Reif WE (eds) (1987) Cytomechanics: the mechanical bases of cell form and structure. Springer, Berlin Heidelberg New YorkGoogle Scholar
  2. 2.
    Boukamp P, Petrussevska RT, Breitkreuz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106: 761–771PubMedCrossRefGoogle Scholar
  3. 3.
    Bowden P, Quinlan R, Breitkreutz D, Fusenig N (1984) Proteolytic modification of acidic and basic keratins during terminal differentiation of mouse and human epidermis. Eur J Biochem 142: 29–36PubMedCrossRefGoogle Scholar
  4. 4.
    Breitkreutz D, Boukamp P, Stark H-J, Ryle C, Fusenig NE (1989) Response of established keratinocyte lines to modulators of epidermal differentiation. In: Reichert U. Shroot B (eds) Pharmacology of retinoids in the skin. (Pharmacology and the skin, vol 3 ). Karger, BaselGoogle Scholar
  5. 5.
    Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19: 1033–1042PubMedCrossRefGoogle Scholar
  6. 6.
    Görmar FE, Bernd A, Bereiter-Hahn J, Holzmann H (1990) A new model of epidermal differentiation: induction by mechanical stimulation. Arch Dermatol Res 282: 22–32PubMedCrossRefGoogle Scholar
  7. 7.
    Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH (1980) Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19: 245–254PubMedCrossRefGoogle Scholar
  8. 8.
    Knapp A, Franke WW, Heid H, Hatzfeld M, Jorcano JL, Moll R (1986) Cytokeratin no. 9, an epidermal type-I keratin characteristic of a special program of keratinocyte differentiation displaying body site specificity. J Cell Biol 103: 657–667PubMedCrossRefGoogle Scholar
  9. 9.
    Maltoltsy AG (1986) Structure and function of the mammalian epidermis. In: Bereiter-Hahn J, Maltoltsy AG, Richards KS (eds) Biology of the integument, vol. 2. Springer, Berlin Heidelberg New YorkGoogle Scholar
  10. 10.
    Moll R, Franke W, Schiller D, Geiger B, Krepier R (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31: 11–24PubMedCrossRefGoogle Scholar
  11. 11.
    Moll I, Heid H, Franke WW, Moll R (1987) Distribution of a special subset of keratinocytes characterized by the expression of cytokeratin 9 in adult and fetal human epidermis of various body sites. Differentiation 33: 254–265PubMedCrossRefGoogle Scholar
  12. 12.
    Ryle C, Breitkreutz D, Stark HJ, Steinert P, Roop D, Fusenig N (1989) Density-dependent modulation of synthesis of keratins 1 and 10 in the human keratinocyte line HaCaT and in RAS-transfected tumorigenic clones. Differentiation 40: 42–54PubMedCrossRefGoogle Scholar
  13. 13.
    Sumpio BE, Banes AJ, Levin LG, Johnson G (1988) Mechanical stress stimulates aortic endothelial cells to proliferate. J Vase Surg 6: 252–256Google Scholar
  14. 14.
    Thie M, Schlumberger W, Rauterberg J, Robeneck H (1989) Mechanical confinement inhibits collagen synthesis in gel-cultured fibroblasts. Eur J Cell Biol 48: 294–302PubMedGoogle Scholar
  15. 15.
    Tseng SGC, Jarvinen MJ, Nelson WG, Huang J-W, Woodcock-Mitchel J, Sun T-T (1982) Correlation of specific keratins with different types epithelial differentiation: monoclonal antibody studies. Cell 30: 361–372PubMedCrossRefGoogle Scholar
  16. 16.
    Vandenburgh HH (1988) Computerized mechanical cell stimulator for tissue culture: effects of skeletal muscle organogenesis. In Vitro Cell Dev Biol 24: 609–619Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • F. E. Görmar
  • A. Bernd
  • J. Bereiter-Hahn
  • H. Holzmann

There are no affiliations available

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