Growth and Differentiation of a Bipotent Precursor of B Cells and Macrophages
Progress in single cell analysis is yielding important new insights concerning the process of B cell development. Single cell cloning assays which distinguish early members of the hemopoietic pathway, some of which are prior to the lymphoid/myeloid branch point, provide the starting material to determine which genetic events are associated with lineage commitment [1, 2]. In this report, we describe a cell which is at or near the time of commitment to the B cell pathway. This cell has not yet undergone Immunoglobulin gene rearrangement and still retains the potential to develop into macrophages as well as B lineage cells . In addition to progress in cellular cloning assays, technical innovations now allow the creation of cDNA libraries from genes expressed in single progenitors . A strategy to identify the genetic events which lead to the commitment process in these cells is presented.
KeywordsCellulose Agar Lymphoma Leukemia Assure
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