Growth and Differentiation of a Bipotent Precursor of B Cells and Macrophages

  • C. J. Paige
  • A. Cumano
  • G. Brady
  • N. N. Iscove
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 182)


Progress in single cell analysis is yielding important new insights concerning the process of B cell development. Single cell cloning assays which distinguish early members of the hemopoietic pathway, some of which are prior to the lymphoid/myeloid branch point, provide the starting material to determine which genetic events are associated with lineage commitment [1, 2]. In this report, we describe a cell which is at or near the time of commitment to the B cell pathway. This cell has not yet undergone Immunoglobulin gene rearrangement and still retains the potential to develop into macrophages as well as B lineage cells [2]. In addition to progress in cellular cloning assays, technical innovations now allow the creation of cDNA libraries from genes expressed in single progenitors [3]. A strategy to identify the genetic events which lead to the commitment process in these cells is presented.


Fetal Liver Single Cell Analysis Founder Cell cDNA Molecule Hemopoietic Progenitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • C. J. Paige
    • 1
  • A. Cumano
    • 1
  • G. Brady
    • 1
  • N. N. Iscove
    • 1
  1. 1.Ontario Cancer Institute, The Wellesley Hospital Research Institute, and Departments of Medical Biophysics and ImmunologyUniversity of TorontoTorontoCanada

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