Growth and Differentiation of a Bipotent Precursor of B Cells and Macrophages
Progress in single cell analysis is yielding important new insights concerning the process of B cell development. Single cell cloning assays which distinguish early members of the hemopoietic pathway, some of which are prior to the lymphoid/myeloid branch point, provide the starting material to determine which genetic events are associated with lineage commitment [1, 2]. In this report, we describe a cell which is at or near the time of commitment to the B cell pathway. This cell has not yet undergone Immunoglobulin gene rearrangement and still retains the potential to develop into macrophages as well as B lineage cells . In addition to progress in cellular cloning assays, technical innovations now allow the creation of cDNA libraries from genes expressed in single progenitors . A strategy to identify the genetic events which lead to the commitment process in these cells is presented.
KeywordsFetal Liver Single Cell Analysis Founder Cell cDNA Molecule Hemopoietic Progenitor
Unable to display preview. Download preview PDF.
- 3.Brady G, Barbara M, and Iscove NN (1990) Representative in vitro cDNA amplification from individual hemopoietic cells and colonies. Meth Mol Cell Biol 2:17–25.Google Scholar
- 11.Lee G, Namen AE, Gillis S, Ellingsworth R, Kincade PW (1984) Normal B cell precursors responsive to recombinant murine IL-7 and inhibition of IL-7 activity by transforming growth factor-β. J Immunol 142:3875–3883.Google Scholar
- 17.Kincade PW (1991) Molecular interactions between stromal cells and B lymphocyte precursors. Sem Immunol 3:379–390.Google Scholar
- 19.Brady G, Iscove NN (1992) Amplified representative cDNA libraries from single cells. Methods in Enzymology, Wassarman PM and DePamphilis ML (eds), in press.Google Scholar
- 26.LeClair KP, Palfree RGE, Flood PM, Hammerling U, Bothwell A (1986) Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J 12:3227–3234.Google Scholar