Injection Techniques in SFC
The objective of the injector is to transfer the whole sample or a predetermined fraction of the sample quantitatively to the column in the narrowest possible band. The first part of this objective, a quantitative transfer of all components, is not always attained by every injection method. Sample discrimination effects which are well known from gas chromatography can be found in SFC as well, but this does not necessarily constitute a problem, as long as the user is aware of the possible appearance of such phenomena. The second part of the objective, transfer in a narrow band, is a process which is affected by numerous factors, such as diffusion, linear velocity, temperature, solvents, loading and mixing efficiency. Some of these factors also influence the first part of the objective, the yield of the transfer process.
KeywordsSupercritical Fluid Extraction Injection Technique Sample Loop Split Injection Dynamic Extraction
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- 1.Greibrokk T, Berg BE, Hohansen H (1988) In: Perrut M (ed) International symposium on supercritical fluids in Nice, October 1988, p 425Google Scholar
- 2.Scott RPW, Simpson CF (1982) J Chromatogr Sci 20:62Google Scholar
- 3.Harvey MC, Stearns SD (1983) J Chromatogr Sci 21:473Google Scholar
- 16.Hirata Y, Tanaka M, Inomata K (1989) J Chromatogr Sci 27:395Google Scholar
- 18.Hawthorne SB, Miller DJ (1989) J Chromatogr Sci 27:197Google Scholar