Supercritical Fluid Chromatography with FT-IR Detection

  • Larry T. Taylor
  • Elizabeth M. Calvey


Both conventional HPLC and GC detectors have proven to be compatible with Supercritical Fluid Chromatography (SFC). For example, flame ionization, electron capture, flame photometric, thermionic, ultraviolet and fluorescence detection [1, 2] have all been successfully demonstrated. Various efforts have been made to couple spectrometric detectors with chromatographic systems in order to gain more specific information regarding eluting components. Fourier Transform infrared (FT-IR) [3], atomic emission, nuclear magnetic resonance and mass [4] spectrometry have been interfaced to SFC with varying degrees of success during the 1980s. The focus of this chapter is SFC/FT-IR. The FT-IR detector is constrained by two major problems: mid-IR absorption by most chromatographically compatible mobile phases and relatively low FT-IR sensitivity compared to some other more established detectors. In order to minimize these problems, various ingenious interface designs have been explored. These designs appear to vary greatly, but they can be classified into two approaches: solvent elimination coupled with transmission or reflectance IR, and flow cell coupled with transmission or attenuated total reflectance IR. Each approach has a unique set of characteristics that makes it attractive for certain applications.


Flow Cell Supercritical Fluid Chromatography Solvent Elimination Capillary Supercritical Fluid Chromatography ZnSe Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Bornhop DJ, Wangsgaard JG (1989) J Chromatogr Sci 27:293Google Scholar
  2. 2.
    Richter BE, Bornhop DJ, Swanson JT, Wangsgaard JG, Anderson MR (1989) J Chromatogr Sci 27:303Google Scholar
  3. 3.
    Taylor LT, Calvey EM (1989) Chem Rev 89:321CrossRefGoogle Scholar
  4. 4.
    Smith RD, Udseth HR (1987) Anal Chem 59:13CrossRefGoogle Scholar
  5. 5.
    French SB, Novotny M (1986) Anal Chem 58:164CrossRefGoogle Scholar
  6. 6.
    Wieboldt RC, Hanna DA (1987) Anal Chem 59:1255CrossRefGoogle Scholar
  7. 7.
    Wieboldt RC (1987) Nicolet FT-IR Application Note AN-8705, March 1987Google Scholar
  8. 8.
    Wieboldt RC, Adams GE, Later DW (1988) Anal Chem 60:2422CrossRefGoogle Scholar
  9. 9.
    Shah S, Ashraf-Khorassani M, Taylor LT (1988) Chromatographia 25:631CrossRefGoogle Scholar
  10. 10.
    Calvey EM, Taylor LT, Palmer JK (1988) J High Resolut Chromatogr Chromatogr Comm 1:294Google Scholar
  11. 11.
    Calvey EM, Roach JAG, Taylor LT, Palmer JK (1989) J Microcol Sep 1:294CrossRefGoogle Scholar
  12. 12.
    Wieboldt RC, Smith JA (1988) ACS Symposium Series 366:229CrossRefGoogle Scholar
  13. 13.
    Shah S, Tkylor LT (1989) J High Resolut Chromatogr 12:599CrossRefGoogle Scholar
  14. 14.
    Ashraf-Khorassani M, Taylor LT (1989) Anal Chem 61:145CrossRefGoogle Scholar
  15. 15.
    Hedrick JL, Calvey EM, Taylor LT (1988) Pittsburgh Conference, New Orleans, LA; February 22–26, paper no 1068Google Scholar
  16. 16.
    Jordan JW, Taylor LT (1986) J Chromatogr Sci 24:82Google Scholar
  17. 17.
    Morin P, Caude M, Rosset R (1987) J Chromatogr 407:87CrossRefGoogle Scholar
  18. 18.
    Shafer KH, Pentoney LL Jr, Griffiths PR (1986) Anal Chem 58:58CrossRefGoogle Scholar
  19. 19.
    Griffiths PR. In: Ferraro JR, Basile LJ (eds) Fourier Transform Infrared Spectroscopy. Academic Press, New York, Vol 1, 143Google Scholar
  20. 20.
    Johnson CC, Taylor LT (1984) Anal Chem 56:2642CrossRefGoogle Scholar
  21. 21.
    Pentoney SL Jr, Shafer KH, Griffiths PR (1986) J Chromatogr Sci 24:230Google Scholar
  22. 22.
    Raymer JH, Moseley MA, Pellizzare ED, Velez GR (1988) J High Resolut Chromatogr Chromatogr Comm 11:209CrossRefGoogle Scholar
  23. 23.
    Fuoco R, Pentoney SL Jr, Griffiths PR (1989) Anal Chem 61:2212CrossRefGoogle Scholar
  24. 24.
    Fujimoto C, Hirata Y, Jinno K (1985) J Chromatogr 332:47CrossRefGoogle Scholar
  25. 25.
    Shafer K, Griffiths PR (1983) Anal Chem 55:1939CrossRefGoogle Scholar
  26. 26.
    Raynor MW, Bartle KD, Davis IL, Williams A, Cliffort AA, Chalmers JM, Cook BW (1980) Anal Chem 60:427CrossRefGoogle Scholar
  27. 27.
    Raynor MW, Davies IL, Bartle KD, Williams A, Chalmers JM, Cook BW (1987) Eur Chromatogr News 1(4): 19Google Scholar
  28. 28.
    Griffiths PR, Pentoney SL, Pariente GL, Norton KL (1988) Mikrochimica Acta Wein, III, 47Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Larry T. Taylor
  • Elizabeth M. Calvey

There are no affiliations available

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