Supercritical Fluid Chromatography with FT-IR Detection
Both conventional HPLC and GC detectors have proven to be compatible with Supercritical Fluid Chromatography (SFC). For example, flame ionization, electron capture, flame photometric, thermionic, ultraviolet and fluorescence detection [1, 2] have all been successfully demonstrated. Various efforts have been made to couple spectrometric detectors with chromatographic systems in order to gain more specific information regarding eluting components. Fourier Transform infrared (FT-IR) , atomic emission, nuclear magnetic resonance and mass  spectrometry have been interfaced to SFC with varying degrees of success during the 1980s. The focus of this chapter is SFC/FT-IR. The FT-IR detector is constrained by two major problems: mid-IR absorption by most chromatographically compatible mobile phases and relatively low FT-IR sensitivity compared to some other more established detectors. In order to minimize these problems, various ingenious interface designs have been explored. These designs appear to vary greatly, but they can be classified into two approaches: solvent elimination coupled with transmission or reflectance IR, and flow cell coupled with transmission or attenuated total reflectance IR. Each approach has a unique set of characteristics that makes it attractive for certain applications.
KeywordsFlow Cell Supercritical Fluid Chromatography Solvent Elimination Capillary Supercritical Fluid Chromatography ZnSe Plate
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- 1.Bornhop DJ, Wangsgaard JG (1989) J Chromatogr Sci 27:293Google Scholar
- 2.Richter BE, Bornhop DJ, Swanson JT, Wangsgaard JG, Anderson MR (1989) J Chromatogr Sci 27:303Google Scholar
- 7.Wieboldt RC (1987) Nicolet FT-IR Application Note AN-8705, March 1987Google Scholar
- 10.Calvey EM, Taylor LT, Palmer JK (1988) J High Resolut Chromatogr Chromatogr Comm 1:294Google Scholar
- 15.Hedrick JL, Calvey EM, Taylor LT (1988) Pittsburgh Conference, New Orleans, LA; February 22–26, paper no 1068Google Scholar
- 16.Jordan JW, Taylor LT (1986) J Chromatogr Sci 24:82Google Scholar
- 19.Griffiths PR. In: Ferraro JR, Basile LJ (eds) Fourier Transform Infrared Spectroscopy. Academic Press, New York, Vol 1, 143Google Scholar
- 21.Pentoney SL Jr, Shafer KH, Griffiths PR (1986) J Chromatogr Sci 24:230Google Scholar
- 27.Raynor MW, Davies IL, Bartle KD, Williams A, Chalmers JM, Cook BW (1987) Eur Chromatogr News 1(4): 19Google Scholar
- 28.Griffiths PR, Pentoney SL, Pariente GL, Norton KL (1988) Mikrochimica Acta Wein, III, 47Google Scholar