Abstract
Acute hepatic failure is a fatal clinical syndrome characterized by progressive jaundice, hemorrhage with prolonged prothrombin time, and severe mental changes progressing to stupor or coma. An effective treatment of acute hepatic failure has not been established. Recently, attention has been given to artificial liver support. Nonbiological artificial liver support, such as hemoperfusion with resin or activated charcoal which adsorb toxic substances, is incomplete, because it has no synthetic functions, namely, synthesis of plasma protein and gluconeogenesis. Biological artificial liver support using excised whole liver [1], liver slices [2], and isolated hepatocytes [3] have synthetic functions but lose them very rapidly. Stabilization of liver functions is necessary to construct an effective artificial liver support system. We have been studying liver functions in immobilized hepatocytes to construct a stable artificial liver support. The present study shows that hepatocytes immobilized within calcium alginate can retain the ability of de novo protein synthesis, normal hormone response, and terminal differentiated state.
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© 1992 Springer-Verlag Berlin Heidelberg
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Miura, Y., Yagi, K. (1992). Characteristics of Hepatocytes Immobilized Within Calcium Alginate and of a Bioreactor for Artificial Liver Support. In: Brunner, G., Mito, M. (eds) Artificial Liver Support. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77359-4_22
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DOI: https://doi.org/10.1007/978-3-642-77359-4_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77361-7
Online ISBN: 978-3-642-77359-4
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