Abstract
DNA restriction fragment length polymorphism (RFLP) analysis has been used more and more in forensic Science on individual identification and paternity test. But the RFLP analysis needs relatively large quantity of undegraded DNA (>300ng for mutilocus probe) and the manupulation is complicated(Jeffreys 1985, 李 伯 龄 1991). In actual cases, the samples to be detected usually contain too little high molecular DNA to conduct RFLP analysis. The development of in vitro DNA amplification provides a new way to solve this Problem. The sequences flanked the target VNTR locus are chosen as primers, and the use of polymerase Chain reaction to amplify the VNTR can result in different length DNA fragment. The polymorphism is called amplified fragment length polymorphism(Amp—FLP)(Bugoele 1991).
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References
Bugoele B, Chakraborty R, et al. (1991) Analysis of the VNTR Locus D1S80 by the PCR followed by high-resolution PAGE. American Journal of Human Genetics. Vol. 48. pp 137–44
Jeffreys A J, etal. (1985) Individual spectific fingerprints of human DNA. Nature 316: 76–79
Kasai R K, et al. (1989) Amplification of VNTR locus by the polymerase chain reaction(PCR). Proceedings of an International Symposium on the Forensic Aspects of DNA Analysis, Govermental Printing Office, Washingten. D. C.
李伯龄等 (1991)α—珠蛋白一 3′ HVR探针 DNA 指纹图法医应用的研究, 遗传 (3) (in press)
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© 1992 Springer-Verlag Berlin Heidelberg
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Boling, L., Yan, D., Jintang, N., Song, C., Lan, H., Jian, Y. (1992). The Amplified Fragment Length Polymorphism Study of Locus pMCT118 and Its Application in Forensic Biology. In: Rittner, C., Schneider, P.M. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77324-2_21
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DOI: https://doi.org/10.1007/978-3-642-77324-2_21
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