PGM1 Subtyping by Isoelectric Focusing (IEF) in Parentage Testing in South African (SA) Populations
PGM1 is an ubiquitous enzyme which is coded for by a gene situated on chromosome 1p. PGM1 reversibly catalyses the transfer of phosphate from the first to the sixth position of glucose in the glycolytic pathway. The polymorphism of PGM1 was first demonstrated by conventional starch gel electrophoresis by Spencer et al in 1964. Using IEF, later studies showed more complex band patterns due to the presence of four common alleles at the PGM1 locus, giving rise to ten possible phenotypes (Bark et al. 1976; Sutton and Burgess 1978). PGM1 is one of thirteen genetic marker systems currently used in disputed paternity testing in this laboratory. The aim of this study was to assess the usefulness of PGM1 subtyping by IEF in disputed parentage testing and compare the results with those obtained using starch gel electrophoresis.
KeywordsStarch Hydroxide Citrate Catalysis Electrophoresis
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