PGM1 Subtyping by Isoelectric Focusing (IEF) in Parentage Testing in South African (SA) Populations

  • V. Borrill
  • W. Petersen
  • R. Martell
  • E. du Toit
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

PGM1 is an ubiquitous enzyme which is coded for by a gene situated on chromosome 1p. PGM1 reversibly catalyses the transfer of phosphate from the first to the sixth position of glucose in the glycolytic pathway. The polymorphism of PGM1 was first demonstrated by conventional starch gel electrophoresis by Spencer et al in 1964. Using IEF, later studies showed more complex band patterns due to the presence of four common alleles at the PGM1 locus, giving rise to ten possible phenotypes (Bark et al. 1976; Sutton and Burgess 1978). PGM1 is one of thirteen genetic marker systems currently used in disputed paternity testing in this laboratory. The aim of this study was to assess the usefulness of PGM1 subtyping by IEF in disputed parentage testing and compare the results with those obtained using starch gel electrophoresis.

Keywords

Starch Hydroxide Citrate Catalysis Electrophoresis 

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References

  1. Bark J, Harris M, Firth M (1976) Typing of the common phosphoglucomutase variants using isoelectric focusing. J Forensic Sci Soc 16: 115–120PubMedCrossRefGoogle Scholar
  2. Spencer N, Hopkinson D, Harris H (1964) Phosphoglucomutase polymorphism in man. Nature 204: 742–745PubMedCrossRefGoogle Scholar
  3. Sutton J, Burgess R (1978) Genetic evidence for four common alleles at the phosphoglucomutase -l - locus (PGM1) detectable by iso-electric focusing. Vox Sang 34: 97–103PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • V. Borrill
    • 1
  • W. Petersen
    • 1
  • R. Martell
    • 1
  • E. du Toit
    • 1
  1. 1.Provincial Laboratory for Tissue ImmunologyCape TownSouth Africa

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