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Detection of Three Different VNTR’s by DNA-Amplification

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Advances in Forensic Haemogenetics

Part of the book series: Advances in Forensic Haemogenetics ((HAEMOGENETICS,volume 4))

Abstract

The Polymerase Chain Reaction (PCR) can enhance the detection of known VNTR loci, particularly in forensic situations where limited amounts of DNA are available. PCR amplification of several polymorphic VNTR regions has been described. Here, we describe the application of the DNA-amplification technique for the detection of three variable minisatellite loci: the VNTR loci D1S80 (D1S58) and D17S30 (D17S5) and the hypervariable region 3′ of the apolipoprotein B gene. Polymorphic PCR-fragmenta were detected on ethidiumbromide stained polyacrylamide gels.

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Literature

  1. Boerwinkle E, Xiong W, Fourest E, Chan L (1989). Rapid typing of tandemly repeated hypervariable loci by the Polymerase Chain Reaction: Application to the apolipoprotein B3 hypervariable region. Proc. Natl. Acad. Sci 86: 212–216.

    Article  PubMed  CAS  Google Scholar 

  2. Ludwig EH, Friedl W & McCarthy BJ (1989). High-Resolution analysis of a hypervariable region in the Human Apolipoprotein B gene. American J. Hum. Genet. 45: 458–464.

    CAS  Google Scholar 

  3. Budowle B, Chakraborty R, Giusty AM, Eisenberg AJ & Allen RC (1991). Analysis of the VNTR Locus D1S80 by the PCR followed by High-Resolution PAGE. Am. J. Hum. Genet. 48: 137–144.

    PubMed  CAS  Google Scholar 

  4. Kasai K, Nakamura Y & White R (1990). Amplification of a variable number of tandem repeats (VNTR) Locus (pMCTI18) by the polymerase chain reaction (PCR) and its application to forensic science. Journal of Forensic Sciences 35: 1196–1200.

    PubMed  CAS  Google Scholar 

  5. Horn GT, Richards B & Klinger KW (1989). Amplification of a highly polymorfic VNTR segment by the Polymerase Chain Reaction. Nucleic Acids Research 17: 2140

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Dutch Forensic Science Laboratory, Volmerlaan 17, 2288 GD, Rijswijk, The Netherlands

    Ate Kloosterman, Rolf Vossen & Deborah Wust

  2. Medscand Ingeny, PO Box 685, 2300 AR, The Netherlands

    Wiljo de Leeuw & André Uitterlinden

Authors
  1. Ate Kloosterman
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  2. Rolf Vossen
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  3. Deborah Wust
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  4. Wiljo de Leeuw
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  5. André Uitterlinden
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Editor information

Editors and Affiliations

  1. Institut für Rechtsmedizin, Johannes Gutenberg-Universität Mainz, Am Pulverturm 3, W-6500, Mainz, Germany

    Christian Rittner  & Peter M. Schneider  & 

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© 1992 Springer-Verlag Berlin Heidelberg

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Kloosterman, A., Vossen, R., Wust, D., de Leeuw, W., Uitterlinden, A. (1992). Detection of Three Different VNTR’s by DNA-Amplification. In: Rittner, C., Schneider, P.M. (eds) Advances in Forensic Haemogenetics. Advances in Forensic Haemogenetics, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77324-2_13

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  • DOI: https://doi.org/10.1007/978-3-642-77324-2_13

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-55194-2

  • Online ISBN: 978-3-642-77324-2

  • eBook Packages: Springer Book Archive

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