Iso-Electric Focusing Study of Serum Proteins (GC, TF, PI and ORM) in Four Endogamous Groups of Maharashtra, Western India: Application in Paternity Testing

  • S. S. Mastana
  • V. Ray
  • S. S. Bhattacharya
  • S. S. Papiha
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)


Iso-electric focusing (IEF) has become an important and powerful technique to discriminate genetic variability for several single gene systems. For example, Transferrin system was found to be monomorphic in most populations with starch gel electrophoresis, but analysis with IEF have clearly shown further microheterogeneity in this system, which makes TF a useful marker for population genetics and paternity determination. Several other serum protein systems HP, PI, GC and BF have shown similar microheterogeneity.


Caste Group Group Specific Component Endogamous Group Gene Frequency Data Southern African Population 
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  1. Chakraborty R, Roychoudhry AK (1975). Paternity exclusion by genetic markers in Indian populations. Ind J Med Res 63: 162–169Google Scholar
  2. Chakraborty R, Shaw MW, Schull, WJ (1974). Exclusion of paternity: The current state of the art. Am Hum Genet 26: 477–488Google Scholar
  3. Constant J, Kühnl P, Viau M, Spielmann W (1980). A new procedure for the determination of tranferrin (TFC) subtypes by isoelectric focusing: existence of two additional alleles, TF4 and TF5. Hum Genet 55: 111–114CrossRefGoogle Scholar
  4. Harpending H C, Jenkins T (1973). Genetic distance among southern African populations. In: Crawford MH and Workman PL (eds), Methods and Theories of anthropological genetics. University of New Mexico Press, pp 177–199Google Scholar
  5. Graber RA, Morris JW (1983). General equations for Average power of exclusion for genetic systems of n codominant allele in one parent and no parent cases of disputed parentage. In: Walker RH (ed), Inclusion probabilities in parentage testing. Am Ass Blood Banks. Arlington (Virg) pp 277–280Google Scholar
  6. Papiha SS, White I, Roberts DF (1983). Some genetic implications of isoelectric focusing of human red cell phosphoglucomutase (PGM1) and serum protein group specific component (GC). Genetic diversity of the populations of Himachal Pradesh, India. Hum Genet 63: 67–72PubMedCrossRefGoogle Scholar
  7. Papiha SS, Pal B, Walker D, Mangion P, Hossain MA (1989). Alpha 1 antitrypsin (PI) phenotypes in two rheumatic diseases: a reappraisal of the association of PI subtypes in rheumatoid arthritis. Ann Rheu Dis 48: 48–52CrossRefGoogle Scholar
  8. Yuasa I, Umsetu K, Suenaga K, Robinet-Levy M (1986). Orosomucoid (ORM) typing by isolelectric focusing: evidence for two structural loci ORM1 and ORM2. Hum Genet 74: 160–161PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • S. S. Mastana
    • 1
  • V. Ray
    • 2
  • S. S. Bhattacharya
    • 3
  • S. S. Papiha
    • 3
  1. 1.Department of Human SciencesLoughborough University of TechnologyLoughboroughUK
  2. 2.KEM Hospital Blood BankBombayIndia
  3. 3.Department of Human Genetics, School of Pathological SciencesNewcastle UniversityNewcastle Upon TyneUK

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