Fast Determination of TF Phenotypes Using Minigel Gradient 4–6.5 Modificated

  • M. De La Iglesia
  • M. A. Martínez-Aguilera
  • A. Gremo
  • J. M. Ruíz De La Cuesta
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 4)

Abstract

Application of minigels and PhastSystem to obtain phenotyping results from blood samples in TF system was proposed by several authors recently. Miniaturized gels disposables commercially offer a number of advantages (nopreparation casting gel), but no usually good resolution pattern bands. Microprcessor PhastSystem offer potential advantages in routine testing: faster separations are possible, 24 samples can be run simultaneously (two minigels), while two others minigels can be stained at the same time.

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References

  1. Carracedo A, and cols. (1990) Fast IEF of some Polymorphic Proteins and Enzymes in Bloodstains Using the PhastSystem. In: Advances in Forensic Haemogenetics 3. Springer-Verlag, Berlin-New york, p 251Google Scholar
  2. Gill P, and cols. (1984) Separation of some Polymorphic Protein Systems of Forensic Significance by Isoelectric Focusing with Separators. Electrophoresis 84: 94–97Google Scholar
  3. Kamboh MI, Ferrell RE (1987) Human Transferrin Polymorphism. Hum. Hered. 37: 65–81PubMedCrossRefGoogle Scholar
  4. Pascali V, Ranalletta D (1982) Improved Typing of Human Serum Transferrin by Isoelectric Focusing on Ultrathin Layer Polyacrylamide Slab Gels. Hum. Genet. 61: 39–41PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. De La Iglesia
    • 1
  • M. A. Martínez-Aguilera
    • 1
  • A. Gremo
    • 1
  • J. M. Ruíz De La Cuesta
    • 1
  1. 1.Department of Legal Medicine, pab. 7Complutense UniversityMadridSpain

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