Abstract
The ability of fish to osmoregulate in freshwater, in seawater and, occasionally, in other salt media, is ensured by the transport activity of several epithelia. Among these tissues, the intestine is also involved in nutrition and the two major functions are largely linked together since the absorption of inorganic ions drives that of small organic solutes and water. In comparison to other organs more complex in morphology, such as gill and kidney, the intestine presents convenient features which make it appropriate for in vitro studies, namely:
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it is “flat”, except for villi and ridges, and may be easily mounted in Ussing chambers,
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its cell population is relatively homogeneous,
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it is possible to isolate viable cells from its mucosa and easy to prepare subcellular fractions, in particular membrane vesicles,
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it is directly accessible to electrophysiological measurements. Accordingly, the following techniques, some classical, others more recent, have been applied:
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everted sac of Wilson and Wiseman, which simultaneously permits measurements of ion and water transport,
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short-circuit current, together with flux measurements, in Ussing chambers,
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short-term apical uptake (measured over 1 min or less), by the method of Schultz et al. (1967),
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isolated cells and apical and basolateral membrane vesicles,
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intracellular recordings by way of microelectrodes (potential-sensitive and ion-selective) and patch-clamp.
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Lahlou, B., Avella, M. (1993). Electrolyte Transport Mechanisms in Fish Intestine. In: Clauss, W. (eds) Ion Transport in Vertebrate Colon. Advances in Comparative and Environmental Physiology, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77118-7_1
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