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Transforming Growth Factors-β in Malignant Glioma: Preliminary Studies on Inhibition by Antisense Oligodeoxynucleotides

  • J. A. Koeppen
  • L. Owen-Schaub
  • E. A. Grimm
  • R. P. Moser
Conference paper
Part of the Advances in Neurosurgery book series (NEURO, volume 20)

Abstract

Type β-transforming growth factors (TGF-β) are multifunctional polypeptides that regulate cell growth and differentiation. TGF-ß1 and TGF-β2 are closely related and resemble the immunosuppressive effects of malignant gliomas. As TGF-ß2 is produced by gliomas, it may contribute to glioma-mediated immunosuppression. To inhibit TGF-β production, a set of 16mer antisense oligodeoxynucleotides (ODNs) was constructed. The glioma cell line U-251 was incubated with 10 μM antisense oligodeoxynucleotides for 24 h. A specific antiproliferative effect was observed for an oligodeoxynucleotide complementary to a region of complete mRNA homology between TGF-ß1 and TGF-ß2. Inhibition of TGF-ß expression could not be verified in a standard Mv-1-Lu bioassay. In this assay TGF-ß inhibits proliferation of Mv-1-Lu cells. Direct treatment of Mv-1-Lu with TGF-ß1 and TGF-ß2 specific antisense as well as unspecific oligodeoxynucleotides resulted in a dose-dependent inhibition of proliferation, indicating direct and unspecific effects of these oligodeoxynucleotides on Mv-1-Lu.

Keywords

Malignant Glioma Glioma Cell Line Lymphokine Activate Killer Antisense Oligodeoxynucleotides Antisense ODNs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • J. A. Koeppen
    • 1
  • L. Owen-Schaub
    • 2
  • E. A. Grimm
    • 2
  • R. P. Moser
    • 2
  1. 1.Neurochirurgische AbteilungUniversitätskrankenhaus EppendorfHamburg 20Germany
  2. 2.M.D. Anderson Cancer CenterUniversity of TexasHoustonUSA

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