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Leukemias pp 15-17 | Cite as

Nucleolar Organizer Regions in Acute Leukemia

  • M. Trněný
  • K. Smetana
Conference paper

Abstract

Nucleolar organizer regions (NORs) are loops of DNA which occur in the cell nucleolus and contain ribosomal RNA genes [1]. These regions can be demonstrated by reaction of NOR protein components with silver [6,9]. In light microscopy they appear as intensely stained brown or black granules called either silver-NORs (Ag-NORs) or silver-stained granules (SSGs). Their number is apparently related to nucleolar biosynthetic activity with respect to ribosomal RNA transcription [9]. The number of SSGs has been studied in metaphases of normal and leukemic bone marrow, and the silver stainability in interphasic leukemic cells has been also investigated in several studies (e.g., 2, 7). There are, however, some differences in the results of these studies. The aim of our study was to provide more information on the number of nucleolar SSGs in interphasic leukemic myeloblasts in the peripheral blood.

Keywords

Acute Myeloid Leukemia Acute Leukemia Silver Staining Nucleolar Organizer Region Human Bone Marrow Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. New York, Garland Publishing, Inc. pp 422–428Google Scholar
  2. 2.
    Arden KC, Bucana CD, Johnston DA, Pathak S (1989) Computer-assisted image analysis of silver staining in normal and leukemic bone marrow. Int J Cancer 43:395–398PubMedCrossRefGoogle Scholar
  3. 3.
    Crocker J, Macartney JC, Smith PJ (1988) Correlation between DNA flow cytometric and nucleolar organizer region data in non-Hodgkin’s lymphomas. J Pathol 154:151–156PubMedCrossRefGoogle Scholar
  4. 4.
    Gavosto F, Pileri A, Bachi C, Pegoraro L (1964) Proliferation and maturation defect in acute leukemia cells. Nature 203:92–94PubMedCrossRefGoogle Scholar
  5. 5.
    Hall PA, Crocker J, Watts A, Stansfeld AG (1988) A comparison nucleolar organizer region staining and Ki-67 immunostaining in non-Hodgkin’s lymphoma. Histo-pathology 12:373–381Google Scholar
  6. 6.
    Likovsky Z, Smetana K (1981) Further studies on the cytochemistry of standardized silver staining of interphase nucleoli in smear preparations of Yoshida ascitic sarcoma cells in rats. Histochemistry 72:301–313PubMedCrossRefGoogle Scholar
  7. 7.
    Mamaev NN, Mamaeva SE, Grabovskaya IL, et al (1987) The activity of nucleolar organizer regions of human bone marrow cells studied with silver staining. II. Acute leukemia. Cancer Genet Cytogenet 25:65–72PubMedCrossRefGoogle Scholar
  8. 8.
    Smetana K, Gyorkey F, Gyorkey P, Busch H (1969) On the ultrastructure of nucleoli in human leukemic myeloblasts. Exp Cell Res 58:303–311PubMedCrossRefGoogle Scholar
  9. 9.
    Smetana K, Busch H (1979) Studies on silver staining components, in Busch H, Croohe ST, Daskal Y (eds): Effects of drugs on the cell nucleus. New York, Academic Press pp 89–105Google Scholar
  10. 10.
    Smetana K, Likovsky Z (1984) Nucleolar silver-stained granules in maturing ery-throid and granulocytic cells. Cell Tissue Res 237:367–370PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • M. Trněný
    • 1
  • K. Smetana
    • 1
  1. 1.Institute of Hematology and Blood TransfusionPragueCzechoslovakia

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