Nucleolar organizer regions (NORs) are loops of DNA which occur in the cell nucleolus and contain ribosomal RNA genes . These regions can be demonstrated by reaction of NOR protein components with silver [6,9]. In light microscopy they appear as intensely stained brown or black granules called either silver-NORs (Ag-NORs) or silver-stained granules (SSGs). Their number is apparently related to nucleolar biosynthetic activity with respect to ribosomal RNA transcription . The number of SSGs has been studied in metaphases of normal and leukemic bone marrow, and the silver stainability in interphasic leukemic cells has been also investigated in several studies (e.g., 2, 7). There are, however, some differences in the results of these studies. The aim of our study was to provide more information on the number of nucleolar SSGs in interphasic leukemic myeloblasts in the peripheral blood.
KeywordsAcute Myeloid Leukemia Acute Leukemia Silver Staining Nucleolar Organizer Region Human Bone Marrow Cell
Unable to display preview. Download preview PDF.
- 1.Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. New York, Garland Publishing, Inc. pp 422–428Google Scholar
- 5.Hall PA, Crocker J, Watts A, Stansfeld AG (1988) A comparison nucleolar organizer region staining and Ki-67 immunostaining in non-Hodgkin’s lymphoma. Histo-pathology 12:373–381Google Scholar
- 9.Smetana K, Busch H (1979) Studies on silver staining components, in Busch H, Croohe ST, Daskal Y (eds): Effects of drugs on the cell nucleus. New York, Academic Press pp 89–105Google Scholar