Eukaryotic Origins of DNA Replication
Initiation of DNA replication acts as a failsafe point in the cell proliferation cycle, because it is a commitment to cell division and, therefore, central to control of the eukaryotic cell cycle (Laskey et al. 1989). In addition, initiation of DNA replication is coupled to programmed changes in gene expression that occur during the life of a single cell as well as during the development of multicellular organisms (Villarreal 1991). Therefore, understanding, at the molecular level, the processes by which DNA replication is initiated is crucial to understanding regulation of cell proliferation, its linkage to gene expression, and its aberrations that lead to diseases such as cancer.
KeywordsReplication Fork Template Strand Autonomously Replicate Sequence DHFR Gene Eukaryotic Origin
Unable to display preview. Download preview PDF.
- Burhans WC, Ortega JM & DePamphilis ML (1992) Origins of DNA replication in eukaryotic cells: What are they and how do they work? Crit Rev Biochem Mol Biol (in press)Google Scholar
- DePamphilis ML, Martínez-Salas E, Cupo DY, Hendrickson EA, Fritze CE, Folk WR & Heine U (1988) Initiation of Polyomavirus and SV40 DNA replication, and the requirements for DNA replication during mammalian development, pp 165–175. In: Stillman B, Kelly T (eds) Eukaryotic DNA replication. Cancer cells, vol 6. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Dijkwel PA, Vaughn JP & Hamlin JL (1990) Mapping of replication initiation sites in mammalian genomes by two-dimensional gel analysis: stabilization and enrichment of replication intermediates by isolation on the nuclear matrix. Mol Cell Biol 11:3850–3859Google Scholar
- Grass C, Gutierrez C, Burhans WC, DePamphilis ML, Koller Th & Sogo JM (1990) Nucleo- some assembly in mammalian cell extracts before and after DNA replication. EMBO J 9:2911–2922Google Scholar
- Guo Z-S & DePamphilis ML (1992) Specific transcription factors stimulate both simian virus 40 and Polyomavirus origins of DNA replication. Mol Cell Biol (in press)Google Scholar
- Gutierrez C, Guo Z-S, Roberts J & DePamphilis ML (1990) Simian virus origin auxiliary sequences weakly facilitate binding of T-antigen, but strongly facilitate initiation of DNA unwinding. Mol Cell Biol 10:1719–1728Google Scholar
- Leu T-H & Hamlin JL (1989) High-resolution mapping of replication fork movement through the amplified dihydrofolate reductase domain in CHO cells by in-gel renaturation analysis. Mol Cell Biol 9:523–531Google Scholar
- Vassilev LT & DePamphilis ML (1992) Guide to identification of origins of DNA replication in eukaryotic cell chromosomes. Crit Rev Biochem Mol Biol (in press)Google Scholar
- Vassilev LT, Burhans WC & DePamphilis ML (1990) Mapping an origin of DNA replication at a single copy locus in exponentially proliferating mammalian cells. Mol Cell Biol 10:4685–4689Google Scholar
- Wirak DO, Chalifour LE, Wassarman PM, Muller WJ, Hasseil JA & DePamphilis ML (1985) Sequence-dependent DNA replication in preimplantation mouse embryos. Mol Cell Biol 5:2924–2935Google Scholar