Regulation of DNA Replication in Escherichia coli

  • E. Boye
  • A. Lyngstadaas
  • A. Løbner-Olesen
  • K. Skarstad
  • S. Wold
Part of the Colloquium der Gesellschaft für Biologische Chemie book series (MOSBACH, volume 43)


The circular chromosome of Escherichia coli is replicated bidirectionally from a unique origin oriC (Master and Broda 1971; Bird et al. 1972). The molecular actors and interactions required for initiation and propagation of a replication fork have to a large extent been characterized and described by biochemical experiments combined with evidence and methods from bacterial genetics and physiology (for reviews, see von Meyenburg and Hansen 1987; Baker and Kornberg 1991; Bremer and Churchward 1991). An oriC-containing plasmid (minichromosome) may be initiated and completely replicated in vitro by purified proteins (Kaguni and Kornberg 1984; Funnell et al. 1986), so that the minimal requirements for minichromosome replication have been defined. In contrast to our detailed knowledge of the biochemical steps of DNA replication, information about regulation of the process remains scant Regulation of DNA replication occurs largely at the level of initiation, but evidence for downstream control points have been presented (Atlung et al. 1987; Skarstad et al. 1989; Løbner-Olesen et al. 1989). This work gives an overview of the factors known or suggested to affect the regulation of DNA replication in E. coli.


Replication Fork Stringent Response Amino Acid Starvation Chromosome Replication DnaA Protein 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • E. Boye
    • 1
  • A. Lyngstadaas
    • 1
  • A. Løbner-Olesen
    • 2
  • K. Skarstad
    • 1
  • S. Wold
    • 1
  1. 1.Department of BiophysicsInstitute for Cancer ResearchMontebelloNorway
  2. 2.Department of Molecular, Cellular, and Developmental BiologyUniversity of ColoradoBoulderUSA

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