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Medicinal and Aromatic Plants IV pp 172–186Cite as

Gentiana Species: In Vitro Culture, Regeneration, and Production of Secoiridoid Glucosides

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 21))

Abstract

Gentiana species belong to the family Gentianaceae, order Gentianales, superorder Gentiananae, subclass Asteridae, class Magnoliopsida (Takhtajan 1980). The species are divided into several sections according to the morphology of the above-ground organs (Sziszkin and Bobrow 1952; Hegi 1966; Tutin et al. 1972). The subgenera Eugentiana Kusnezow and Gentianella Kusnezow (Hegi 1966) are entered in Flora Europaea as separate genera: Gentiana L. and Gentianella Moench (Tutin et al. 1972).

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References

  • Bajaj YPS, Furmanowa M, Olszowska O (1988) Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 60–103

    Google Scholar 

  • Bakuridze AD, Dargaeva TD, Patudin AV, Efremow AP, Nikolaeva GG, Brutko LI, Rabinowicz AM (1987a) Metod opredelenija sodierzanija sumy iridoidow u Gentiana. Rast Resur 23: 455–458

    CAS  Google Scholar 

  • Bakuridze AD, Dargaeva TD, Nikolaeva GG, Patudin AV, Brutko LI (1987b) Iridoids in plants of the genus Gentiana (Gentianaceae). Chim Prirod Soedin 1: 3–11 (in Russian)

    Google Scholar 

  • Barešová H (1988) Centaurium erythraea Rafn: micropropagation and the production of sec-oiridoid glucosides. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 350–366

    Google Scholar 

  • Barralis G, Chadoeuf R, Desmarêst P (1978) New trends ofGentiana lutea cultivation. Acta Hortic 73: 303–306

    Google Scholar 

  • Bellmann G, Jacot-Guillarmod A (1973a) Contribution â la phytochimie du genre Gentiana. I. Etude des composes flavoniques et xanthoniques dans les feuilles de Gentiana lutea L. Helv Chim Acta 56: 284–294

    Article  CAS  Google Scholar 

  • Bellmann G, Jacot-Guillarmod A (1973b) Contribution â la phytochimie du genre Gentiana. II. Etude des cyclitols dans les feuilles de Gentiana lutea L. Helv Chim Acta 56: 773–775

    Article  CAS  Google Scholar 

  • Bue TG, Krys ZOP (1975) K biologii prorastanija semjon gorecavki zeltoj. Biul Dl Bötan Sada AN URSS Nr 96: 52–55; RefZ-Biol 1975 Nr 11: w 410

    Google Scholar 

  • Canonica L, Pelizzoni F, Manito P, Jommi G (1961) Structure of gentiopicroside. Tetrahedron 16: 192–200

    Article  CAS  Google Scholar 

  • Chialva F, Frattini C, Martelli A (1986) Öl von Gentiana lutea. Z. Lebensm Unters Forsch 182: 212–214

    Article  CAS  Google Scholar 

  • Dragendorff G (1898) Die Heilpflanzen der verschidenen Völker und Zeiten. Enke, Stuttgart, pp 529–530

    Google Scholar 

  • Dombrowicz E, Swiatek L (1987) Kwasy fenolowe w surowcach goryczkowych. Farmacja Pol 43: 639–642

    Google Scholar 

  • El-Sedawy A, Hattori M, Kobashi K, Namba T (1989) Metabolism of gentiopicroside (gentiopicrin) by human intestinal bacteria. Chem Pharm Bull 37: 2435–2437

    PubMed  CAS  Google Scholar 

  • Franz C, Fritz D (1975) Anbauversuche mit Gentiana lutea und Inhaltsstoffe einiger Ökotypen. Planta Med 28: 289–300

    Article  PubMed  CAS  Google Scholar 

  • Franz C, Fritz D (1978) Cultivation aspects of Gentiana lutea L. Acta Hortic 73: 307–312

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Garcia J, Chulia A J (1986) Loganin and new iridoid glucosides inGentiana pedicellata. Planta Med 4: 327–329

    Article  PubMed  Google Scholar 

  • Gawlowska J (1986) Pierwsze polowe uprawy goryczki zôltej w Polsce. Wiad Zielar 27(6): 1–3

    Google Scholar 

  • Ghosal S, Singh AAK, Biswas K (1983) New 6-aryl-2 pyrones from Gentiana pedicellata. Planta Med 49: 240–243

    Article  PubMed  CAS  Google Scholar 

  • Hayashi T, Yamagishi T (1988) Two xanthone glycosides from Gentiana lutea. Phytochemistry 27: 3696–3699

    Article  CAS  Google Scholar 

  • Hegi G (1966) Illustrierte Flora von Mitteleuropa, Bd 3. Hanser, München, pp 1979–2047

    Google Scholar 

  • Hegnauer R (1966) Chemotaxonomie der Pflanzen, Bd 4. Birkhäuser, Basel, pp 176–192

    Google Scholar 

  • Heymons S, Hölzl J, Weber HC (1986) Va-Mycorrhiza in Gentiana lutea, the importance of cultivation and influence on constituents. Planta Med 34th Annu Congr Medicinal Plant Research, Hamburg, p 510

    Google Scholar 

  • Hostettmann K, Wagner H (1977) Xanthone glycosides. Phytochemistry 16: 821–829

    Article  CAS  Google Scholar 

  • Hostettmann K, Bellmann G, Tabacchi R, Jacot-Guillarmad A (1973) Contribution â la phytochimie du genre Gentiana. Ill Etude des composes flavoniques et xanthoniques dans les feuilles de Gentiana lutea L. Helv Chim Acta 56: 3050–3054

    Article  CAS  Google Scholar 

  • Index Kewensis Plantarum Phanerogmarum 1895, Suppl I-XVIII (1906–1987) University Press, Oxford

    Google Scholar 

  • Inouye H, Nakamura Y (1971) Über die Monoterpenglucoside und verwandte Naturstoffe XIV. Die struktur der beiden stark bitter schmeckenden Glucoside Amarogentin und Amaroswerin ausSwertia japónica. Tetrahedron 27: 1951–1966

    Article  CAS  Google Scholar 

  • Inouye H, Yoshida T, Nakamura Y, Tobita S (1968) Die Stereochemie einiger Secoiridoid- glucoside und die Revision der Struktur des Gentiopicrosids. Tetrahedron Lett 42: 4429–4432

    Article  Google Scholar 

  • Inouye H, Ueda S, Nakamura Y (1970) Über die Monoterpenglucoside X. Secoiridoid-glucoside aus Swertia japónica. Isolierung von fünf Secoiridoid-glucosiden sowie die Strukturaufklärung des Swerosids, des Swertiamarins und des Gentiopicrosids. Chem Pharm Bull 18: 1856–1865

    CAS  Google Scholar 

  • Inouye H, Ueda S, Nakamura Y, Inoue K, Hayano T, Matsumura H (1974) Über die Monoterpenglucoside und verwandte Naturstoffe XXIV, Triflorosid ein neues Secoiridoidglucosid aus Gentiana triflora var. japónica. Tetrahedron 30: 571–577

    Article  CAS  Google Scholar 

  • Ishiguro K, Yamaki M, Takagi S (1983) Studies on iridoid-related compounds. III. Gentiopicral, the aglucone of gentiopicroside. Planta Med 49: 208–210

    Article  PubMed  CAS  Google Scholar 

  • Isiguro K, Yamaki M, Takagi S, Ikeda Y, Kawakami K, Ito K, Nose T (1986) Studies on iridoid-related compounds. IV. Antitumor activity of iridoid aglycones. Chem Pharm Bull 34: 2375–2379

    PubMed  CAS  Google Scholar 

  • Koshioka M, Takino Y, Hayshi T (1980) Studies on the evaluation of crude drugs. X. II Quantitative determination of bitter compounds in Swertiae Herba. Planta Med 38 (Suppl): 64–67

    Article  Google Scholar 

  • Kozlowski J (1983) Goryczka zólta - wartosciowa roslina lecznicza. Wiad Zielar 25(10–11): 16–17

    Google Scholar 

  • Krupińska A, Skrzypczak L (1989) Oznaczanie amarogentyny metoda densytometryczna. XIV Naukowy Zjazd Polsk Towarzystwa Farm. Wroclaw, Streszczenia, p 7/30

    Google Scholar 

  • Krupińska A, Segiet-Kujawa E, Skrzypczak L, Ellnain-Wojtaszek M (1991) Quantitative determination of amarogentin by TLC densitometry. Sci Pharm 59: 135–138

    Google Scholar 

  • Lamproye A, Crevecoeur M, Kevers C, Gaspar Th (1987) Multiplication vegetative in vitro de Gentiana lutea et de Gentiana pneumonanthe. Med Fac Landbuoww Rijkuniv Gent 52: 1255–1257

    Google Scholar 

  • Luo JP, Lou ZC (1986) TLC-densitometry determination of bitter glycosides in Chinese drug Longdan, Radix Gentianae and its quality evaluation. Yaoxue Xuebao 21: 40–46; I Ph A (1987) 2411788

    CAS  Google Scholar 

  • Maiwald L (1987) Phytotherapie bei Erkrankungen des Magen-Darm-Traktes. Z Phytother 8: 184–185

    Google Scholar 

  • Menković N, Ralić J, Savin K, Ivanic R (1986) Prilog farmakognozijskom proueavanju nekoliko Gentiana - vrsta. Farm Glas 42: 127–135

    Google Scholar 

  • Meravy L (1987) Phenolic substances in tissue cultures of Centaurium erythraea. Biol Plant 29: 81–87

    Article  CAS  Google Scholar 

  • Miura H (1991) Swertia spp.:in vitro culture, regeneration, and production of secondary metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 15. Medicinal and aromatic plants III. Springer, Berlin Heidelberg New York, pp 451–463

    Google Scholar 

  • Mpondo EM, Garcia J (1990) Two iridoid glucosides from Gentiana verna. Phytochemistry 29: 643–644

    Article  Google Scholar 

  • Mpondo EM, Garcia J, Chulia A J (1990a) Secoiridoid glucosides from Gentiana campestris. Phytochemistry 29: 1687–1688

    Article  Google Scholar 

  • Mpondo EM, Garcia J, Cartier G, Pellet G (1990b) 6’-O-ß-D-glucosylswertiamarine: a new secoiridoid from Gentiana alpina. Planta Med 56: 334

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nikolaeva GG, Glyzin WI, Mladencewa MS (1983) Xanthones of Gentiana lutea. Chim Prirod Soedin 1: 107–108 (in Russian)

    Google Scholar 

  • Nikolaev SM, Bakuridze AD, Dargaeva TD, Brutko LI, Patudin AV, Schagzhieva GA, Nikolaeva GG (1987) Prospects of the utilisation of the overground part of Gentiana lutea L. for medicinal preparations. Izv Sib Otd Akad Nauk URSS Ser Biol Nauk 0(3): 67–70 (in Russian)

    Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87

    Article  PubMed  CAS  Google Scholar 

  • Paun E, Mihalea A (1977) Probleme actúale si de perspectiva privint plántele medicínale si aromatice. Prod Vegetala Cereale Plante Technice, Bucuresti 29(7): 28–31

    Google Scholar 

  • Penso G (1983) Index Plantarum Medicinalium Totius Mundi Eorumque Synonymorum. OEMF, Milano, pp 436–437

    Google Scholar 

  • Pierik RLM (1989) In vitro culture of higher plants. Nijhoff, Dordrecht, pp 305–308

    Google Scholar 

  • Procyk A (1982) Mozliwosci uprawy goryczki zoltej. Wiad Zielar 24(1–2): 7–8

    Google Scholar 

  • Quercia V, Pierini N, Battaglino G (1980) Study of bitter constituents of the gentian root by high pressure liquid chromatography. Acta Hortic 96: 231–244

    Google Scholar 

  • Rote Liste (1986) Verzeichnis von Fertigarznei-mitteln der Mitglieder des Bundesverbandes der Pharmazeutischen Industrie. Cantor, Aulendorf Württ

    Google Scholar 

  • Sakamoto I, Morimoto K, Tanaka O, Inouye H (1983) Application of high performance liquid chromatography and field desorption mass spectroscopy to separative analysis of bitter secoiridoid glucosides of Swertiae Herba. Chem Pharm Bull 31: 25–30

    CAS  Google Scholar 

  • Schaufelberger D, Hostettmann K (1985) Analytical and preparative reversed-phase liquid chromatography of secoiridoid glycosides. J Chromatogr 346: 396–400

    Article  CAS  Google Scholar 

  • Schaufelberger D, Domon B, Hostettmann K (1984) Desorption chemical ionization mass spectrometry of bitter glycosides from Gentiana. Planta Med 50: 398–403

    Article  PubMed  CAS  Google Scholar 

  • Schultze J, Franz C (1980) Sugar, bitter substances and essential oils ofGentiana lutea L. In dependence of ecotype, plant age and stage of development. Acta Hortic 96: 311–315

    Google Scholar 

  • Semeniuk P, Griesbach RJ (1987) In vitro propagation of prairie gentian. Plant Cell Tissue Organ Cult 8: 249–253

    Article  CAS  Google Scholar 

  • Skrzypczak L, Wesolowska M (1989) Blackstonia perfoliata (L.) Hudson i Eustoma grandiflorum Shinn. z kultur in vitro. XIV Naukowy Zjazd Polsk Towarzystwa Farm. Wroclaw, Streszczenia, p 7/42; 48 Zjazd Polsk Towarzystwa Botan, Katowice, Streszczenia, p 129

    Google Scholar 

  • Skrzypczak L, Wesolowska M, Thiem B (1988a) Callus culture and micropropagation ofEustoma grandiflorum Shinn. Acta Soc Bot Pol 57: 423–430

    Google Scholar 

  • Skrzypczak L, Wesolowska M, Thiem B (1988b) Callus culture and micropropagation ofEustoma grandiflorum and Blackstonia perfoliata. 5th Int Conf Biology of cell cultures and biotechnology, Novosibirsk, Poster session

    Google Scholar 

  • Sticher O, Meier B (1978) Identifizierung der Bitterstoffe von Radix Gentianae mit Hochleistungs-Flüssigkeitschromatographie (HPLC). Pharm Acta Helv 53: 40–45

    PubMed  CAS  Google Scholar 

  • Sticher O, Meier B (1980) Quantitative Bestimmung der Bitterstoffe in Wurzeln von Gentiana lutea undGentiana purpurea mit HPLC. Planta Med 40: 55–67

    Article  CAS  Google Scholar 

  • Swiatek L, Dombrowicz E, Zadernowski R (1988) Zawartosc fenolokwasöw w korzeniach goryczki zoltej (Gentiana lutea L.). Herba Pol 34: 15–20

    CAS  Google Scholar 

  • Sziszkin BK, Bobrow EG (eds) (1952) Flora URSS, vol 18. AN URSS, Moscow Leningrad, pp 538–620

    Google Scholar 

  • Takhtajan AI (1980) Outline of the classification of flowering plants (Magnoliophyta). Bot Rev 46: 225–359

    Article  Google Scholar 

  • Tutin TG, Heywood VH, Burges NA, Moore DA, Valentine DH, Walters SM, Webb DA (1972) Flora Europaea, vol 3. University Press, Cambridge, pp 59–63

    Google Scholar 

  • Van der Sluis WG, Labadie RP (1981) Fungitoxic activity of the secoiridoid glucoside gentiopicrin (gentiopicroside). Planta Med 42: 139–140

    Article  PubMed  Google Scholar 

  • Van der Nat J, van der Sluis WG, Labadie RP (1982) Gentiogenal, a new antimicrobial iridoid derived from gentiopicrin (gentiopicroside). Planta Med 45: 161–162

    PubMed  Google Scholar 

  • Van der Sluis WG, Van der Nat JM, Spik AL, Tkeshiro Y, Labadie RP (1983) Gentiogenal, a conversion product of gentiopicrin (gentiopicroside). Planta Med 49: 211–215

    Article  PubMed  Google Scholar 

  • Vanhaelen M, Vanhaelen-Fastre R (1983) Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography densitometry. J Chromatogr 281: 263–271

    Article  CAS  Google Scholar 

  • Verney AM, Debelmas AM (1973) Sur diverses xanthones deGentiana lutea L., G. purpurea L., G. punctata L., G. pannonica Scop. Ann Pharm Fr 31: 415–420

    PubMed  CAS  Google Scholar 

  • Verotta L (1985) Isolation and HPLC determination of the active principles of Rosmarinus officinalis andGentiana lutea. Fitoterapia 56: 25–29

    CAS  Google Scholar 

  • Viola U, Franz C (1989) In vitro propagation of Gentiana lutea. 37th Annu Congr on Medicinal plant research, Abstracts of short lectures and poster presentations, Braunschweig, p 115

    Google Scholar 

  • Wagner H, Vasirian K (1974) Zur Chromatographie der Enzianbitterstoffe. Dtsch Apoth Ztg 114: 1245–1248

    CAS  Google Scholar 

  • Wagner H, Müjizing-Vasirian K (1975) Eine chemische Wertbestimmung der Enziandroge. Dtsch Apoth Ztg 115: 1233–1239

    CAS  Google Scholar 

  • Wagner H, Bladt S, Zgainski EM (1984) Plant drug analysis. Springer, Berlin Heidelberg New York, pp 125–136

    Google Scholar 

  • Weiss RF (1980) Lehrbuch der Phytotherapie. Hippocrates, Stuttgart, pp 54–57

    Google Scholar 

  • Weiss RF (1988) Phytotherapie bei Magenbeschwerden, Aerztezeitschr Naturheilverf 29:429–436

    Google Scholar 

  • Wenzel H (1972) Seltene Pflanzen in der Drogenkammer. Alkohol Ind 85: 24–25

    Google Scholar 

  • Wesolowska M, Skrzypczak L, Dudzinska R (1985) RodzajGentiana L. w kulturze in vitro. Acta Pol Pharm 42: 79–83

    CAS  Google Scholar 

  • Wichtl M (1978) Drogenanalytik und Arzneibuch kritisch betrachtet. Planta Med 34: 113–128

    Article  PubMed  CAS  Google Scholar 

  • Zenkteler M (ed) (1984) Hodowla komörek i tkanek roslinnych. PWN, Warszawa, pl35

    Google Scholar 

  • Zenkteler M, Zenkteler E (1987) RozmnazanieEustoma grandiflorum za pomoca kultur in vitro. Ogrodnictwo 24: 19–21

    Google Scholar 

  • Zimmermann, W, Gaisbauer G, Gaisbauer M (1986) Wirkung von Bitterstoff-Drogen auf das darmassozüerte Immunsystem. Z Phytother 7: 56–64

    Google Scholar 

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  1. Department of Pharmaceutical Botany, K. Marcinkowski Medical Academy, Wieniawskiego 1, 61-712, Poznań, Poland

    E. Skrzypczak

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  1. L. Skrzypczak
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  2. M. Wesolowska
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  3. E. Skrzypczak
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  1. A-137, New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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Skrzypczak, L., Wesolowska, M., Skrzypczak, E. (1993). Gentiana Species: In Vitro Culture, Regeneration, and Production of Secoiridoid Glucosides. In: Bajaj, Y.P.S. (eds) Medicinal and Aromatic Plants IV. Biotechnology in Agriculture and Forestry, vol 21. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77004-3_12

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