Abstract
Cloned origin-rich sequences (ors) isolated by strand extrusion from mammalian (monkey and human) cells are capable of transient autonomous semiconservative replication in transfected HeLa cells. Sequence analyses reveal no primary consensus sequence features that can be associated with origin function, but several common structural features have been noted which include A/T-rich stretches of DNA and inverted repeats that can potentially give rise to cruciform structures. Some similarities to the yeast ARS consensus, the consensus for attachment to nuclear scaffold and for other regulatory elements have also been noted. The data suggest that origin activation is most probably dependent on the presence of structural determinants rather than on primary sequence.
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© 1992 Springer-Verlag Berlin Heidelberg
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Zannis-Hadjopoulos, M., Pearson, C.E., Bell, D., Mah, D., McAlear, M., Price, G.B. (1992). Structural and Functional Characteristics of Autonomously Replicating Mammalian Origin-Enriched Sequences (ORS). In: Hughes, P., Fanning, E., Kohiyama, M. (eds) DNA Replication: The Regulatory Mechanisms. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76988-7_10
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DOI: https://doi.org/10.1007/978-3-642-76988-7_10
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