Genetic Determinants for Microcin H47, an Escherichia Coli Chromosome-Encoded Antibiotic

  • M. Laviña
  • C. Gaggero
Part of the NATO ASI Series book series (volume 65)


A search for antibiotic activities produced by Gram-negative bacteria from Uruguay was performed. Strains isolated from different natural sources were screened and 23 out of 170 were able to inhibit the growth of an E. coli K12 indicator strain. Antibiotic activities were typed following the criteria of Baquero and Moreno (1984). They were classed as colicins when their production was induced with mitomycin, and as microcins when they were not mitomycin-inducible and were able to pass through a cellophane membrane which allows the passage of molecules smaller than 7.000 Da. Thus, fifteen strains producing colicins and eight strains producing microcins were isolated


Genetic Determinant Antibiotic Production Antibiotic Activity Complementation Group Phage Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Baquero F, Moreno F (1984) The microcins. FEMS Microbiol Lett 23:117–124CrossRefGoogle Scholar
  2. Garrido MC, Herrero M, Kolter R, Moreno F (1988) The export of the DNA replicator inhibitor microcin B17 provides immunity for the host cell. EMBO J 7:1853–1862PubMedGoogle Scholar
  3. Genilloud O, Kolter R, Moreno F (1989) DNA sequence, products, and transcriptional patterns of the genes involved in production of the DNA replication inhibitor microcin B17. J Bacteriol 171:1126–1135PubMedGoogle Scholar
  4. Groisman EA, Casadaban MJ (1986) Mini-Mu bacteriophage with plasmid replicons for in vivo cloning and lac gene fusing. J Bacteriol 168:357–364PubMedGoogle Scholar
  5. Lavina M, Gaggero C, Moreno F (1990) Microcin H47, a chromosome-encoded microcin antibiotic of Escherichia coli. J Bacteriol 172:6585–6588Google Scholar
  6. Novoa MA, Diaz-Guerra L, San Millân JL, Moreno F (1986) Cloning and mapping of the genetic determinants for microcin C7 production and immunity. J Bacteriol 168:1384–1391PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. Laviña
    • 1
  • C. Gaggero
    • 1
  1. 1.División de Biología MolecularInstituto de Investigaciones Biológicas C.EstableMontevideoUruguay

Personalised recommendations