In the evolution of life systems, water has been a component of transcendent importance. In addition to its involvement in syntheses and metabolism, it has provided the cradle supporting the structures of macromolecules, and it has been the main source of the forces which generate and maintain membrane structures. The ability to survive the removal of water in anhydrous systems is especially remarkable as a departure from these structural dependencies. Because desiccation tolerance is widespread through the plant and animal kingdoms, we may hope that it might involve relatively simple biochemical modifications (Green and Angell 1989). In this chapter we will suggest that the basis for the survival of desiccation involves the accumulation of solutes which can lead to a vitrification of the cytoplasm as desiccation proceeds.
KeywordsSugar Crystallization Sucrose Corn Hydration
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- Bruni F (1990) Anhydrous biology and glass transitions. In: Stanley HE, Ostrowsky N (eds) Correlations and Connectivity. Kluwer Academic Publishers, pp 272–275Google Scholar
- Bruni F, Williams RJ, Leopold AC (1990) Vitrified cytoplasm in dry seeds. Plant Physiol 93: 779Google Scholar
- Burke MJ (1986) The glassy state and survival of anhydrous biological systems. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, pp 358–363Google Scholar
- Crowe JH, Crowe LM (1986) Stabilization of membranes in anhydrobiotic organisms. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, pp 188–209Google Scholar
- Crowe LM, Womersley C, Crowe JH, Reid D, Appel L, Rudolph A (1986) Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates. Biochem Biophys Acta 861: 131–140Google Scholar
- Labuza TP (1984) Moisture Sorption. Am Assoc Cereal Chem, St Paul, 150 ppGoogle Scholar
- Takahashi T, Hirsh A, Erbe E, Williams RJ (1988) Cryoprotection by extracellular polymeric solutes.Biophys J 54: 509–518Google Scholar
- Vertucci CW, Leopold AC (1986) Physiological activities associated with hydration level in seeds. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, pp 35–49Google Scholar
- Webb SJ (1965) Bound water in biological integrity. Thomas, Springfield, DE, 187 ppGoogle Scholar