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A Role for the 73-kDa Heat Shock Cognate Protein in a Lysosomal Pathway of Intracellular Protein Degradation

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Heat Shock

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Abstract

The Increased lysosomal degradation of cytosolic proteins during serum withdrawal is stimulated by a member of the 70-kDa heat shock protein (HSP701) family. This HSP70, designated the peptide recognition protein of 73 kDa (prp73), can be isolated by affinity chromatography with RNase S-peptide-Sepharose, and it stimulates lysosomal uptake and degradation of [3H]RNase S-peptide in vitro. Prp73 binds to several proteins and peptides that contain peptide regions biochemically related to amino acids 7–11 of RNase S-peptide, Lys-Phe-Glu-Arg-Gln (KFERQ).

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Abbreviations

HSP70s:

heat shock proteins of the 70 kDa family

HSC73:

heat shock cognate protein of 73 kDa

grp78:

glucose-regulated protein of 78 kDa

RNase A:

bovine pancreatic ribonuclease A

RNase S-peptide:

residues 1-20 of RNase A

RNase S-protein:

residues 21-124 of RNase A

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Author information

Authors and Affiliations

  1. Department of Physiology, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA, 02111, USA

    J. F. Dice, H.-L. Chiang, S. R. Terlecky & T. S. Olson

  2. Department of Biochemistry, University of California, Berkeley, CA, 94720, USA

    H.-L. Chiang

Authors
  1. J. F. Dice
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  2. H.-L. Chiang
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  3. S. R. Terlecky
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  4. T. S. Olson
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Editor information

Editors and Affiliations

  1. International Institute of Genetics and Biophysics, Via Marconi 12, 80125, Naples, Italy

    Bruno Maresca

  2. Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, The University of Chicago, 1103 E 57th Street, 60637, Chicago, IL, USA

    Susan Lindquist

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© 1991 Springer-Verlag Berlin Heidelberg

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Dice, J.F., Chiang, HL., Terlecky, S.R., Olson, T.S. (1991). A Role for the 73-kDa Heat Shock Cognate Protein in a Lysosomal Pathway of Intracellular Protein Degradation. In: Maresca, B., Lindquist, S. (eds) Heat Shock. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76679-4_20

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  • DOI: https://doi.org/10.1007/978-3-642-76679-4_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76681-7

  • Online ISBN: 978-3-642-76679-4

  • eBook Packages: Springer Book Archive

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