Evaluation of Variable Number of Tandem Repeat (VNTR) Alleles in Mother-Child Combinations
One problem in using VNTR polymorphism for forensic applications is to decide whether two bands should be considered to represent the same DNA fragment. To examine the variation in the assignment of fragment sizes DNA from 189 mother-child pairs tested in adjacent lanes were compared. The size of fragments detected by YNH24 was calculated by an image processing system. The difference between fragments shared by mother and child was 0.004 ± 0.43% with a maximum range of 2.7%. According to these results the fragments in mother-child pairs can differ by ± 1.5% and still give a confidence of 99.97% that the mother and child have a fragment in common. These window limits excluded no mothers but falsely included 1.2% additional fragments.
KeywordsMigration Agarose Electrophoresis Nylon
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