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Evaluation of Variable Number of Tandem Repeat (VNTR) Alleles in Mother-Child Combinations

  • G. Holmlund
  • B. Lindblom

Abstract

One problem in using VNTR polymorphism for forensic applications is to decide whether two bands should be considered to represent the same DNA fragment. To examine the variation in the assignment of fragment sizes DNA from 189 mother-child pairs tested in adjacent lanes were compared. The size of fragments detected by YNH24 was calculated by an image processing system. The difference between fragments shared by mother and child was 0.004 ± 0.43% with a maximum range of 2.7%. According to these results the fragments in mother-child pairs can differ by ± 1.5% and still give a confidence of 99.97% that the mother and child have a fragment in common. These window limits excluded no mothers but falsely included 1.2% additional fragments.

Keywords

Fragment Size Image Processing System Forensic Application Child Pair Adjacent Lane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Holmlund G, Lindblom B (1990) VNTR polymorphism: reproducibility in techniques and interpretation. In: Polesky HF, Mayr WR (eds) Advances in forensic haemogenetics 3. Springer, Berlin Heidelberg New York, pp 34–36Google Scholar
  2. 2.
    Lindblom B, Holmlund G (1988) Rapid DNA purification for restriction fragment length polymorphism analysis. Gene Anal Techn 5:97–101CrossRefGoogle Scholar
  3. 3.
    Nakamura Y, Gillilan S, O’Connell P, Leppert M, Lathrop GM, Lauel J-M, White R (1987) Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44). Nucleic Acids Res 15:10073PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • G. Holmlund
  • B. Lindblom
    • 1
  1. 1.State Institute for Blood Group SerologyUniversity HospitalLinköpingSweden

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