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Chemiluminescence: Nucleic Acid Detection for the Future

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Rapid Methods and Automation in Microbiology and Immunology

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Abstract

Nucleic acid hybridization assays have led to major advances in several research fields. These have provided benefits in our understanding of the organization and expression of prokaryotic and eukaryotic genomes as well as improved technology for the diagnosis of genetic and infectious diseases. At present, the vast majority of DNA and RNA probes used in these areas are radioactively labeled, most commonly with 32P. Probes labeled in this way exhibit excellent detection sensitivity, but have a short half-life and present the safety and disposal issues associated with radioactive reagents. These problems have contributed to the slow transition of nucleic acid probe technology from the research setting to more routine laboratories. The aim of this chapter is to describe alternative, non-radioactive detection methods, with particular emphasis on the potential of chemiluminescence in nucleic acid probe assays.

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Author information

Authors and Affiliations

  1. Amersham International plc, White Lion Road, Amersham, Buckinghamshire, HP7 OHJ, UK

    L. M. F. Proudfoot

Authors
  1. M. R. Evans
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  2. L. C. A. Benge
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  3. A. T. Devenish
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  4. I. Durrant
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  5. S. J. Fowler
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  6. E. R. Harding
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  7. R. Howe
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  8. T. C. Richardson
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  9. G. Scozzafava
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  10. C. Sturrock
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  11. L. M. F. Proudfoot
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Editor information

Editors and Affiliations

  1. Department of Virology, University of Helsinki, Haartmaninkatu 3, SF-00290, Helsinki, Finland

    Antti Vaheri

  2. North American Laboratory Group, 1 Lake Street, 06052, New Britain, CT, USA

    Richard C. Tilton

  3. 105 Bay Colt Road, 30201, Alpharetta, GA, USA

    Albert Balows

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© 1991 Springer-Verlag Berlin Heidelberg

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Evans, M.R. et al. (1991). Chemiluminescence: Nucleic Acid Detection for the Future. In: Vaheri, A., Tilton, R.C., Balows, A. (eds) Rapid Methods and Automation in Microbiology and Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76603-9_2

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  • DOI: https://doi.org/10.1007/978-3-642-76603-9_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76605-3

  • Online ISBN: 978-3-642-76603-9

  • eBook Packages: Springer Book Archive

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