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Mutagenesis of the Mouse Germline Using Retroviruses

  • Chapter
Retroviral Insertion and Oncogene Activation

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 171))

Abstract

Traditionally, mutations in the mouse have been identified as spontaneously occurring mutations in feral or laboratory-maintained mice or as induced mutations in radiation-or chemical-treated mice (GREEN 1981). Although much has been learned from the study of these mutations, molecular analysis of the mutated genes has often been hampered by the lack of molecular access to them. To circumvent this problem, transposable elements can be used as insertional mutagens. The presence of a transposable element within or near the mutant gene acts as a molecular tag which aids in cloning the mutated gene of interest. This approach has been used with great success in several organisms, most notably Drosophila melariogaster (Rubin 1988).

L.F.L. is supported by a Damon Runyon-Walter Winchell Cancer Fund Fellowship, DRG-918. This work was sponsored by the National Cancer Institute, DHHS, under contract N01-CO-74101 with ABL.

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Authors and Affiliations

  1. Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, 21702, Frederick, MD, USA

    L. F. Lock, N. A. Jenkins & N. G. Copeland

Authors
  1. L. F. Lock
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  2. N. A. Jenkins
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  3. N. G. Copeland
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Editor information

Editors and Affiliations

  1. Dept. of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, 2019 Adelbert Rd., 44106, Cleveland, OH, USA

    Hsing-Jien Kung

  2. Dept. of Microbiology, School of Medicine, University of Southern California, 2011 Zonal Avenue HMR-401, 90033-1054, Los Angeles, CA, USA

    Peter K. Vogt

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© 1991 Springer-Verlag Berlin Heidelberg

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Lock, L.F., Jenkins, N.A., Copeland, N.G. (1991). Mutagenesis of the Mouse Germline Using Retroviruses. In: Kung, HJ., Vogt, P.K. (eds) Retroviral Insertion and Oncogene Activation. Current Topics in Microbiology and Immunology, vol 171. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76524-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-76524-7_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76526-1

  • Online ISBN: 978-3-642-76524-7

  • eBook Packages: Springer Book Archive

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