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Cyclic-GMP Phosphodiesterase in Photoreceptor Cells in Limulus Ventral Eye

  • M. Inoue
  • K. Ackermann
  • J. E. Brown
Part of the Research Reports in Physics book series (RESREPORTS)

Abstract

The cyclic-GMP phosphodiesterase (PDE) in Limulus ventral eyes was found to have the following characteristics. The PDE required magnesium (or manganese ions) for maximal activity. The Km for cyclic-GMP was 0.5 μM. Light diminished the PDE activity; the Light/Dark ratio was about 0.79 (for 2 × 10−4 W/cm2 steady illumination). Addition of 0.01 mM GTP or GTP γ S increased the effect of illumination. The light-induced diminution was blocked by 0.1 mM GDP β S. These data suggests that light modulates cyclic-GMP PDE through a G-protein in Limulus ventral eyes.

Keywords

Guanylate Cyclase Phosphodiesterase Activity Photoreceptor Membrane Vertebrate Photoreceptor Steady Illumination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • M. Inoue
    • 1
  • K. Ackermann
    • 2
  • J. E. Brown
    • 2
  1. 1.Department of OphthalmologyKobe UniversityKobeJapan
  2. 2.Department of OphthalmologyWashington UniversitySt. LouisUSA

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