Micropropagation of Strawberry (Fragaria spp.)

  • A. Martinelli
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 18)

Abstract

The strawberry is a herbaceous, perennial member of the family Rosaceae. The garden strawberry, Fragaria × ananassa Duchesne, originated in Europe around 1750 as a hybrid between the pistillate South American F. chiloensis Duch. and a North American F. virginiana Duch. For many centuries before, however, strawberries had been a favorite among the fruits of the temperate world. They were valued for delicious flavor and fragrance, for health-restoring qualities, and as the “harbinger of spring”. (Wilhelm and Sagen 1974).

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adams AN (1972) An improved medium for strawberry meristem culture. J Hortic Sci 47: 263–264Google Scholar
  2. Anderson HM (1984) A useful variant in micropropagated Cambridge Vigour plants. L. Ashton Annu Rep 1984: 65Google Scholar
  3. Anderson HM, Abbott AJ, Wiltshire S (1982) Micro-propagation of strawberry plants in vitro - effect of growth regulators on incidence of multi-apex abnormality. Sci Hortic 16: 331–341CrossRefGoogle Scholar
  4. Anon (1986) Certificazione Sanitaria Volontaria Regione Emilia-Romagna. LR 34/82; Bollettino uff. No 89 hi 30/7/86Google Scholar
  5. Asahira T Kano Y (1977) Shoot formation from cultured tissue of strawberry fruit. J Jpn Soc Hortic Sci 46: 317CrossRefGoogle Scholar
  6. Barrientos F, Bringhurst RS (1973). A haploid of an octoploid strawberry. Hort Science 8: 44–45.Google Scholar
  7. Beech MG, Crisp CN, Simpson SE, Atkinson D (1988) The effect of in vitro cytokinin concentration on the fruiting and growth of conventionally propagated strawberry runner progeny. J Hortic Sci 63 (1): 77–81Google Scholar
  8. Belkengren RO, Miller PW (1962) Culture of apical meristems of Fragaria vesca strawberry plants as a method of excluding latent A virus. Plant Disease Rep 46: 119–121Google Scholar
  9. Boxus P (1974) The production of strawberry plants by in vitro micro-propagation. J Hortic Sci 49: 209–210Google Scholar
  10. Boxus P (1989) Review of in vitro strawberry mass production. In: Acta Hortic Proc Int Strawberry Symp, Cesena, It, May 22–27, 1988, 265: 309–320Google Scholar
  11. Boxus P, Larvor P. (1987) Proc Cost 87 (EEC) Worksh in vitro culture of strawberry plants. Brussels, Dec 1984, EEC, Luxembourg pp 1–100Google Scholar
  12. Boxus P, Quoirin M, Laine MJ (1977) Large scale propagation of strawberry plants from tissue culture. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin, Heidelberg New York, pp 130–143Google Scholar
  13. Cameron JS, Hancock JF, Nourse TM (1985), The field performance of strawberry nursery stock produced originally from runners or micropropagation. Strawberry Product 4: 56–58Google Scholar
  14. Converse RH (1984) Virus diseases of small fruits. USD A Agricultural handbook, Washington Damiano C (1980) Strawberry micropropagation. In: Proc Conf Nursery production of fruit plants through tissue culture. Applications and feasibility. April 21–23, Beltsville MD, pp 11–22Google Scholar
  15. Darrow GM (1966) The strawberry history, breeding and physiology. Holt, Rinehart Winston, New YorkGoogle Scholar
  16. Desjardins Y, Gosselin A, Yelle S (1987) Acclimatization of ex vitro strawberry plantlets in C02-enriched environment and supplementary lighting J. Am Soc Hortic Sci 112 (5): 846–851Google Scholar
  17. Fabbri A, Sutter E, Dunston SK (1986) Anatomical changes in persistent leaves of tissue-cultured strawberry plants after removal from culture. Sci Hortic 28 (4): 331–337CrossRefGoogle Scholar
  18. Faedi W, Rosati P, D’ercole N (1989) The strawberry breeding program for Nort Italy. In: Acta Hortic; Int Strawberry Symp, Cesena, May 22–27, 1988, 265: 53–60Google Scholar
  19. FAO (ed) (1987) Production yearbook. FAO Statist Ser N 76. FAO, RomeGoogle Scholar
  20. Fowler CW, Hughes HG, Janick J (1971) Callus formation from strawberry anthers. Hortic Res 11: 116–117Google Scholar
  21. Fujiwara K, Kozai T, Watanabe I (1988) Development of a photoautotrophic tissue culture system for shoot and/or plantlets at rooting and acclimatization stages. Acta Hortic 230: 153–158Google Scholar
  22. Galletta GJ (1981a) Breeding strawberries for disease resistance. In: Childers NF (ed) The strawberry; cultivars to marketing. Horticultural Publications, Gainesville, FL, pp 193–200Google Scholar
  23. Galletta GJ, (1981b) Strawberry breeding in the United States in the last 10 years: progress and objectives In: Childers NF (ed) The strawberry; cultivars to marketing. Horticultural Publications, Gainesville, FL, pp 135–148Google Scholar
  24. Galletta GJ, Draper AD, Maas, JL (1989) Combining disease resistance, plant adaptation and fruit quality in breeding short-day and day-neutral strawberries. In: Acta Hortic Int Strawberry Symp, Cesena, It, May 22–27, 1988, 265: 43–52Google Scholar
  25. Glimelius K, Djupsjobacka M, Fellner-Feldegg H (1986) Selection and enrichment of plant protoplast heterokaryons of Brassicacea by flow sorting. Plant Sci 45: 133–141CrossRefGoogle Scholar
  26. Gresshoff PM, Doy CH (1972). Development and differentiation of haploid Lycoprersicum esculentum (tomato). Planta 107: 161–170CrossRefGoogle Scholar
  27. Grout BWW, Millam S (1985). Photosynthetie development of micropropagated strawberry plantlets following transplanting. Ann Botany (London) 55 (1): 129–131Google Scholar
  28. Grout BWW, Taffs J, Donkin ME (1986) Sucrose-independent strawberry cultures - an indication of future developments in micropropagation. 6th IAPTC Congr, Minnesota (Abstr), p 148Google Scholar
  29. Hennerty MJ, Douglas AJ, Sayegh AJ (1987) Studies on androgenesis of the strawberry. In: Proc Ir Bot Meet, Cork, 25–27 March, 1987, pp 76–80Google Scholar
  30. Hilton RE, Smith SH, Frazier NW, Schlehel DE (1970). Elimination of mild yellow edge virus from Fresno and J6 strawberry varieties by a combination of heat treatment and meristem tip culture. Strawberry News Bull 16 (6): 1–2Google Scholar
  31. Howell PJ, Rankin P (1984) Red core on Strawberry: importance and certification in Scotland. In: EPPO Spec Meet Strawberry red cored disease, Harpenden, GB, 1–2 Feb 1983, EPPO Bull 14: 910–913Google Scholar
  32. Hughes HG, Janick J (1974) Production of tetrahaploids in cultivated strawberry. Hort Science 9: 442–444Google Scholar
  33. Hunter SA, Foxe MJ, Hennerty MJ (1983) The influence of temperature and light intensity on the in vitro propagation of the strawberry (Fragaria x ananassa Duch) cv. Cambridge Favourite. Acta Hortic 131: 153–161Google Scholar
  34. Izsak E, Izhar S (1983) Rapid micropropagation of strawberry plantlets from seeds for breeding purposes. Acta Hortic 131: 101–103Google Scholar
  35. James DJ, Newton B (1977) Auxin: cytokinin interaction in the in vitro micropropagation of strawberry plants. Acta Hortic 78: 321–331Google Scholar
  36. James DJ, Jones OP, Beech MG, Baker SA, Carter D, Crisp CM, Grout LJ, Hadlow WCC, Passey AJ, Waller BJ (1987). Micropropagation and plant improvement by somacloning and genetic manipulation. Rep E Mailing Res Stn 1986: 116–117Google Scholar
  37. Jones OP (1987) Micropropagation of strawberry and temperate fruit trees. In: Alderson PG, Dullforce WM (eds) Micropropagation in horticulture, practice and commercial problems. Proc Inst Hortic Symp, Nottingham, March 1986, pp 84–96Google Scholar
  38. Jones OP, Waller BJ, Beech MG (1988) The production of strawberry plants from callus cultures. Plant Cell Tissue Org Cult 12: 235–241CrossRefGoogle Scholar
  39. Jungnickel F (1988) Strawberries (Fragaria spp. and hybrids). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6: Crops II. Springer, Berlin Heidelberg New York, pp 38–103Google Scholar
  40. Kano Y, Asahira T (1978) Effects of some growth regulators on the development of strawberry fruits in vitro culture. J Jpn Soc Hortic Ser 47: 195CrossRefGoogle Scholar
  41. Kartha KK, Gamborg OL, Shyluk JP, Constabel F (1976) Morphogenetic investigations on in vitro leaf culture of tomato (Lycopersicon esculentum Mill. cv. Starfire) and high frequency plant regeneration. Z Pflanzenphysiol 77: 292–301Google Scholar
  42. Kartha KK, Leung NL, Paul K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J Am Soc Hortic Sci 105 (4): 481–484Google Scholar
  43. Kennedy BW, King TH (1962) Angular leaf spot of strawberry caused by Xanthomonas Fragariae sp. nov. Phytopathology 52: 873–875Google Scholar
  44. Kinet JM, Parmentier A (1989) Flowering behaviour of micropropagated strawberry plants. In: Acta Hortic Int Strawberry Symp, Cesena, May 22–27, 1988, 265: 327–334Google Scholar
  45. Laneri U, Damiano C (1981) Strawberry anther culture. Ann 1st Sper Frutticolt 1980:43–48Google Scholar
  46. Lee ECM, De Fossard RA (1975) Regeneration of strawberry plants from tissue cultures. Proc Int Plants Soc 25: 277Google Scholar
  47. Lee ECM, De Fossard RA (1977) Some factors affecting multiple bud formaion of strawberry (:Fragaria x ananassa Duch) in vitro. Acta Hortic 78: 187–195Google Scholar
  48. Lembright HW (1981) Soil fumigation for strawberries. In: Childers NF (ed) The strawberry; cultivars to marketing, Horticultural Publications, Gainesville, FL, pp 229–237Google Scholar
  49. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  50. Lis PK (1974) Uptake and metabolism of sucrose - 14C and IAA-1-14C in strawberry fruit expiants cultivated in vitro. In: Proc 19th Int Hortic Congr l.A (Abstr), p 61.Google Scholar
  51. Maas JL (ed) (1984) Compendium of strawberry diseases. Am Phytopathol Soc, St. Paul, MNGoogle Scholar
  52. Malone RP, Dix PJ (1986) Selection for herbicide resistance in tissue cultures of Fragaria and Nicotiana In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworths, London 479–486Google Scholar
  53. Marcotrigiano M, Swartz H J, Gray SE, Tokarcik D, Popenoe J (1984) The effect of benzylamino purine on the in vitro multiplication rate and subsequent field performance of tissue culture-propagated strawberry plants. Adv Strawberry Prod 3: 23–25Google Scholar
  54. Martinelli A (in press). Advantages of automated propagation of fruit trees. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 8: Scale-up and automation in plant propagation.Google Scholar
  55. McGrew JR (1965) Eradication of latent C virus in the Suwannee variety of strawberry by heat plus excised runner tip culture. Phytopathology 55: 480–481Google Scholar
  56. Miller RW, Belkengren RO (1963) Elimination of yellow edge, crinkle, and vein banding viruses and certain other virus complexes from strawberries by excision and culturing of apical meristems. Plant Disease Rep 47: 298–300Google Scholar
  57. Molot PM, Leroux JP, Nouriseau JG (1972) Regénération par culture d’apex de clones de fraisiers infectés de façon chronique par le Phytophthora cactorum. CR 3. Congr Un Phytopathol Mediter, Lisbonne, pp 87–92Google Scholar
  58. Mullin RH, Schlegel DE (1976) Cold storage maintenance of strawberry meristem plantlets. Hort Science 11 (2): 100–101Google Scholar
  59. Mullin RH, Smith SH, Frazier NW, Schlegel DE, McCall SR (1974) Meristem culture frees strawberries of mild yellow edge, pallidosis, and mottle diseases. Phytopathology 64: 1425–1429CrossRefGoogle Scholar
  60. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  61. Nauman WD, Synowski B (1979) Wuchs und Ertrag. Lei = stung von Erdbeerpelanzen nach Mikro Vermehrung. Erwerbsobstbau 21: 142–146Google Scholar
  62. Navatel JC (1979) La multiplication in vitro du fraisier. CTIFL Doc 60Google Scholar
  63. Navatel JC, Varachaud G, Roudeillac P, Barbet A (1985) Comportement agronomique de plantes dont les pieds mères ant été obtenus par multiplication in vitro par rapport ou matériel classique. In: Rep CIREF-CTIFL Cent Balandran, Bellegarde, FrGoogle Scholar
  64. Navatel JC, Nourriseau JG, Rancillac M (1989) Field behaviour of strawberry plants issued from micropropagation: 7 years of experimental studies (1981–1987). In: Proc COST 87 Worksh Strawberry micropropagation, May 19, 1988, Forli EECGoogle Scholar
  65. Niemirowicz-Szczytt K (1984) Morphological and cytological evaluation of progeny obtained from pollination of Fragaria x ananassa with Potentilla pp. Acta Soc Bot Pol 53: 455–468Google Scholar
  66. Niemirowicz-Szczytt K (1990a) Somaclonal variation in Strawberry. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 11: Somaclonal variation in crop improvement I. Springer, Berlin Heidelberg New York, pp 511–528Google Scholar
  67. Niemirowicz-Szczytt K (1990b) Strawberry (Fragaria x ananassa Duch.): in vitro production of haploids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12: Haploids in crop improvement I. Springer, Berlin Heidelberg New York, pp 403–416Google Scholar
  68. Niemirowicz-Szczytt K, Zakrzewska Z, Malepszy S, Kubicki B (1983). Characters of plants obtained from Fragaria x ananassa in anther culture. Acta Hortic 131: 231–237Google Scholar
  69. Nillson E (1989) Performance of strawberry plants after different micropropagation treatments. In: Proc COST 87. Worksh Strawberry micropropagation. May 19, 1988 Forli EECGoogle Scholar
  70. Nishi S, Oosawa K (1973) Mass production method of virus-free strawberry plants through meristem callus. Jpn Agric Res 7: 189–194Google Scholar
  71. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87PubMedCrossRefGoogle Scholar
  72. Nyman M, Wallin A (1987) Culture and plant regeneration of strawberry mesophyll protoplast. In: 7th Protoplast Congr, Wageningen. Dec 1987 ( Abstr )Google Scholar
  73. Oosawa K, Takayanagi K (1982) High yielding variants in strawberry derived from anthers culture. Plant tissue culture 1982. Maruzen, Tokyo, pp 765–766Google Scholar
  74. Penneil D (1987) Strawberry micropropagation within the UK. In: Boxus P, Larvor P (eds). Proc COST 87 (EEC) Workshop In vitro culture of strawberry plants, Brussels, Dec 1984, EEC -Luxembourg, pp 27–34Google Scholar
  75. Plakidas AG (1964) Strawberry diseases. Louisiana State Univ Press, Baton RougeGoogle Scholar
  76. Posnette AF, Cropley R (1958) Heat treatment for the inactivation of strawberry viruses. J Hortic Sci 32: 282–288Google Scholar
  77. Posnette AF, Jha A (1960) The use of cuttings and heat treatment to obtain virus-free strawberry plants. East Mailing Res Stn Rep 1959Google Scholar
  78. Predieri S, Fasolo Fabbri Malavasi F, Ancherani M (1989) Plant regeneration from unpollinated strawberry (Fragaria x ananassa Duch) ovaries and petals. In: Acta Hortic Int Strawberry Symp, Cesena, May 22–27, 1988, 265: 335–342Google Scholar
  79. Rosati P, Devreux M, Laneri U (1975) Anther culture of strawberry. Hort Science 10 (2): 119–120Google Scholar
  80. Rosati P, Gaggioli D, Lovato A, Mezzetti B (1990) Screening for Phytophthora cactorum resistance with cultural filtrate of the fungus. In: Acta Hortic Int Strawberry Symp, Cesena May 22–27, 1988, 265: 123–128Google Scholar
  81. Sansavini S, Corelli L (1983) Aspetti genetici della produzione del materiale vivaistico. In: Il vivaismo in frutticoltura, Garda Jun 9, 1983, pp 37–56Google Scholar
  82. Sansavini S. Gherardi G (1980) Selezione clonale e stabilità genetica di fragole micropropagate Frutticultura 55, 5: 39–46Google Scholar
  83. Sansavini S, Brighenti G, Camorani F (1982) Variazioni fenotipiche residuali indotte da propagazione in vitro su le cultivar di fragola in vivaio e in pieno campo. Frutticultura 44: 71–81Google Scholar
  84. Sarwar M (1984) The effect of different media and culture techniques on plating efficiency of strawberry mesophyll cells in culture. Physiol Plant 60: 57–60CrossRefGoogle Scholar
  85. Sayegh AJ, Hennerty MJ. Androgenesis and embryo rescue for strawberry haploid production. In: Acta Hortic Int Strawberry Symp, Cesena, May 22–27, 1988, 265: 129–136Google Scholar
  86. Scott DH, Zanzi C (1981) Rapid propagation of strawberry from meristems. In: Childers NF (ed) The strawberry, cultivars to marketing. Horticultural Publications, Gainesville, FL, pp 213–221Google Scholar
  87. Scott DH, Galletta GJ, Swartz HJ (1985) Tissue culture as an aid in the propagation of Tribute everbearing strawberry. Adv Strawberry Prod 4: 56–60Google Scholar
  88. Scott DH, Galletta GJ, Bringhurst RS (1989) Progress in strawberry production and research in the USA. In: Acta Hortic Int Strawberry Symp, Cesena, May 22–27, 1988, 265: 637–646Google Scholar
  89. Seemuller E, Merkle F (1984) Eliminierung von Phytophthora fragariae Durk Meristenkultur. Gartenbauwissenschaft 49 (5/6): 227–230Google Scholar
  90. Shoemaker NP, Swartz HJ, Galletta GJ (1985) Cultivar dependent variation in pathogen resistance due to tissue culture propagation of strawberries. Hort Science 20: 253–254Google Scholar
  91. Simon I, Racz E, Zatyko JM (1987) Preliminary notes on somaclonal variations of strawberry. Fruit Sci Rep 14–4: 151–154Google Scholar
  92. Simpson DW, Beech MG (1987) Strawberry breeding in the United Kingdom. In: Abbot AJ, Atkin RK (eds) Improving vegetatively propagated crops. Academic Press, New York, London, pp 149–158Google Scholar
  93. Sobczykiewicz D (1979) Heat treatment and meristem culture for the production of virus-free strawberry plants. Acta Hortic 95: 79–81Google Scholar
  94. Spiegel S (1987) Double stranded RNA in strawberry plants infected with strawberry mild yellow edge virus. Phytopathology 77: 1492–1494CrossRefGoogle Scholar
  95. Stenger DC, Mullin RH, Morris TJ (1987) Characterization and detection of the strawberry necrotic shock isolate of tobacco streak virus. Phytopathology 77: 1330–1337CrossRefGoogle Scholar
  96. Swartz MJ, Lindstrom JT (1986) Small fruit and grape tissue culture from 1980 to 1985: commercialization of the technique. In: Zimmerman RH. (eds) Tissue culture as a plant production system for horticultural crops. Nijhoff, Dordrecht, pp 201–220Google Scholar
  97. Swartz HJ, Galletta GJ, Zimmerman RH (1981) Field performance and phenotypic stability of tissue culture-propagated strawberries. J Am Soc Hortic Sci 106 (5): 667–673Google Scholar
  98. Swartz HJ, Lindstrom JT, Fiola J A (1987) The use of tissue culture propagation of strawberry in the USA, In: Boxus P. Larvor Proc COST (EEC) Worksh In vitro culture of strawberry plants. Brussels, Dec 1984, EEC, Luxembourg, pp 79–100Google Scholar
  99. Theiler-Hedtrich R, Wolfensberger H (1987) Comparison of plant and yield characters of in vitro and normal propagated strawberry plants. Acta Hortic 212: 445–448Google Scholar
  100. Vine SJ (1968) Improved culture of apical tissue for production of virus-free strawberries. J Hortic Sci 43: 293–197Google Scholar
  101. Wang D, Wergin WP, Zimmerman RH (1984) Somatic embryogenesis and plant regeneration from immature embryos of strawberry. Hort Science 19 (1): 71–72Google Scholar
  102. White PR (1954) The cultivation of animal and plant cells. Ronald, New York. 239 ppGoogle Scholar
  103. Wilhelm S, Nelson RD (1981) Fungal diseases of the strawberry plant. In: Childers NF (ed) The strawberry cultivars to marketing. Horticultural Publications, Gainesville, FL, pp 247–299Google Scholar
  104. Wilhelm S, Paulus AO (1980) How soil fumigation benefits the California strawberry industry. Plant Disease 64 (3): 264–170Google Scholar
  105. Wilhelm S Sagen JE (1974) A history of the strawberry, from ancient gardens to modern markets. Div Agric Sci, Univ Cal, Berkeley, pp 3–10Google Scholar
  106. Xue G, Kaiwei F, Jun H (1981) Obtaining haploid plants of strawberry (Fragaria orientalis, Los) by anther culture in vitro. Acta Hortic Sin 8: 4–12Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • A. Martinelli
    • 1
  1. 1.Consorzio Italiano VivaistiS. Giuseppe di Comacchio (Ferrara)Italy

Personalised recommendations