Skip to main content
SpringerLink
Log in
Book cover

High-Tech and Micropropagation I pp 32–52Cite as

Micropropagation Through Meristem Culture

  • Chapter

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 17))

Abstract

Isolation and growth of the shoot meristem are of value in recovering pathogen-free plants. When vegetatively propagated plants are systemically infected with disease, the pathogen passes from one vegetative generation to the next. The entire population of a given clonal variety may be infected with the same pathogen. With latent viruses, the symptoms may be as difficult to detect as reduced yield or lower plant quality. Such infections can be difficult to eliminate.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams RM II, Koenigsberg SS, Langhans RW (1979) In vitro propagation of Cephalotiis follicularis ( Australian pitche plant ). HortScience 14: 512–513

    Google Scholar 

  • Alleweldt G, Harst-Langenbucher M (1987) The effect of growth inhibitors on long-term storage of in vitro cultures of grapevine. Vitis 26 (2): 57–64

    CAS  Google Scholar 

  • Aminuddin Singh BP (1985) Petunia hybrida Hort, explant culture: effect of virazole and certain dyestuffs on Petunia mosaic virus elimination. Indian Phytopathol 38:692–694

    Google Scholar 

  • Ancora G, Belli-Donini ML, Cuozzo L (1981) Globe artichoke plants obtained from shoot apices through rapid in vitro micropropagation. Sei Hortic 14: 207–213

    CAS  Google Scholar 

  • Appiano A, Pennazio S (1972) Electron microscopy of potato meristem tips infected with potato virus X. J Gen Virol 14: 273–276

    PubMed  CAS  Google Scholar 

  • Bajaj YPS (1977a) Clonal multiplication and cryopreservation of cassava through tissue culture. Crop Improv 4: 198–204

    Google Scholar 

  • Bajaj YPS (1977b) Initiation of shoots and callus from potato tuber sprouts and axillary buds frozen at -196 °C. Crop Improv 4: 48–53

    Google Scholar 

  • Bajaj YPS (1979) Freeze preservation of Arachis hypogaea and Cicer arietinum. Indian J Exp Biol 17: 1405

    Google Scholar 

  • Bajaj YPS (1981) Regeneration of plants from potato meristems freeze-preserved for 24 months. Euphytica 30: 141–145

    Google Scholar 

  • Bajaj YPS (1983) Cassava plants from meristem cultures freeze-preserved for 3 years. Field Crops Res 7 (3): 161–167

    Google Scholar 

  • Bajaj YPS (1985) Cryopreservation of germplasm of potato (Solanum tuberosum L.) and cassava {Manihot esculenta Crantz): viability of excised meristems cryopreserved up to 4 years. Indian J Exp Biol 23: 285–287

    Google Scholar 

  • Bajaj YPS (1986) In vitro preservation of genetic resources - techniques and problems. Int Symp Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 43–57

    Google Scholar 

  • Bajaj YPS, Dhanju MS (1979) Regeneration of plants from apical meristem tips of some legumes. Curr Sei 48: 906–907

    Google Scholar 

  • Bajaj YPS, Gill MS (1986) Micropropagation and germplasm preservation of cotton (Gossypium spp.) through shoot tip and meristem culture. Ind J Exp Biol 24: 581–583

    Google Scholar 

  • Bockholt S, Hellwald KH, Buchenauer H (1989) Untersuchungen zum Wirkingsspektrum und zur Wirkungsweise zweier quarternarer Ammoniumverbindungen gegenüber phytopathogenen Viren. J Phytopathol 127: 331–340

    CAS  Google Scholar 

  • Boonnour K, Wainwright H, Hicks TGT (1988) The micropropagation of Lonicera perielymenum L. (honeysuckle). Acta Hortic 226: 183–189

    Google Scholar 

  • Borissenko S, Schuster G, Schmygla W (1985) Obtaining a high percentage of explants with negative seriological reactions against viruses by combining potato meristem culture with antiphytoviral chemotherapy. Phytopathol Z 114: 185–188

    CAS  Google Scholar 

  • Brainerd KE, Fuchigami LH, Kwiatkowshi S, Clark CS (1981) Leaf anatomy and water stress of aseptically cultured Pixy plum grown under different environments. HortScience 16: 173–175

    Google Scholar 

  • Broome OC, Zimmerman RH (1978) In vitro propagation of blackberry. HortScience 13: 151–153

    Google Scholar 

  • Cassells AC, Long RD (1980) The regeneration of virus-free plants from cucumber mosaic virus and potato virus Y-infected tobacco plants cultured in the presence of virazole. Z Naturforsch 35Q350–351

    Google Scholar 

  • Cassells AC, Long RD (1982) The elimination of potato viruses X, Y, S and M in meristem and explant cultures of potato in the presence of virazole. Potato Res 25: 165–173

    Google Scholar 

  • Chu IYE (1986) The application of tissue culture to plant improvement and propagation in the ornamental horticulture industry. In: Zimmerman RH, Griesbach RJ, Hammerschlag FA, Lawson RH (eds) Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff, Boston, pp 15–33

    Google Scholar 

  • Csinos A, Hendrix W (1977) Toxin produced by Phytophythora cryptogea active on excised tobacco leaves. Can J Bot 55: 1156–1162

    CAS  Google Scholar 

  • Cutter EG (1971) Plant anatomy: experiment and interpretation. Part 2. Addison-Wesley London, p 343 deFossard RA, Nitsch C, Cresswell RJ, Lee ECM (1974) Tissue and organ culture of Eucalyptus. NZ J For Sei 4: 267–278

    Google Scholar 

  • Dereuddre J, Galerne M, Gazeau C (1987) The effects of sucrose on survival of freezing in liquid nitrogen (-196 °C) of carnation {Dianthus caryophyllus L.) apical meristems cultivated in vitro. CR Acad Sei Paris 304 (19): 485–487

    CAS  Google Scholar 

  • Druart P (1985) True-to-type propagation of cherry rootstocks and varieties by in vitro culture. Acta Hortic 169: 319–328

    Google Scholar 

  • Esau K (1960) Anatomy of seed plants, 1st edn. Wiley Interscience, New York, p 376

    Google Scholar 

  • Fedotina VL, Krylova NV (1976) Ridding tobaccos of the mycoplasmic infection big bud by the method of tissue culturing. Dokl Bot Sei 228: 49–51

    Google Scholar 

  • Gamborg OL, Miller LA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    PubMed  CAS  Google Scholar 

  • George EF, Sherrington PD (1984) Commercial tissue culture laboratories. In: George EF, Sherrington PD (eds) Plant propagation by tissue culture - handbook and directory of commercial laboratories. Eastern Press, Great Britain, pp 549–551

    Google Scholar 

  • Glendening TM, Sjolund T (1988) In vitro propagation of kohlrabi from leaf explants. HortScience 23 (4): 772

    Google Scholar 

  • Griesbach RJ, Semenluk P (1987) Use of somaclonal variation in the improvement of Eustoma grandiflorum. J Hered 78: 114–116

    Google Scholar 

  • Grout BW, Henshaw G (1978) Freeze-preservation of potato shoot tip culture. Ann Bot 42: 1227–1229

    Google Scholar 

  • Grout BW, Westcott R, Henshaw G (1978) Survival of shoot meristems of tomato seedlings frozen in liquid nitrogen. Cryobiology 15: 478–483

    PubMed  CAS  Google Scholar 

  • Gupta PK, Mascarenhas AF, Jagannathan V (1981) Tissue culture of forest trees - clonal propagation of mature trees of Eucalyptus citriodora Hook, by tissue culture. Plant Sei Lett 20: 195–201

    Google Scholar 

  • Hackett WP (1969) Aseptic multiplication of lily bulblets from bulb scales. Proc Int Plant Prop Soc 19: 105–108

    Google Scholar 

  • Hasegawa PM (1980) Factors affecting shoot and root initiation from cultured rose shoot tips. J Am Soc Hortic Sei 105: 216–220

    CAS  Google Scholar 

  • Hasegawa PM, Murashige T, Takatori FH (1973) Propagation of Asparagus through shoot apex culture. II. Light and temperature requirements, transplantability of plants, and cytohistological characteristics. J Am Soc Hortic Sei 98: 143–148

    Google Scholar 

  • Haskins RH, Kartha KK (1980) Freeze-preservation of pea meristems: cell survival. Can J Bot 58: 833–840

    Google Scholar 

  • Hollings M, Stone OM (1964) Investigation of carnation viruses. I. Carnation mottle. Ann Appl Biol 53: 103–118

    Google Scholar 

  • Hollings M, Stone OM (1965) Investigation of carnation viruses. II. Carnation ringspot. Ann Appl Biol 56: 73–86

    Google Scholar 

  • Hollings M, Stone OM (1968) Techniques and problems in the production of virus-tested planting material. Sei Hortic 20: 57–72

    Google Scholar 

  • Huth W, Bode O (1970) Befreiung Völlig infizierter Kartoffelsorten von Infektionen des Kartoffel-SVirus durch Meristemkultur. Nachrichtenbl Dtsch Pflanzenschutdienst (Braunschw) 22: 37–39

    Google Scholar 

  • Hwang SC, Chen CL, Lin JC, Lin HL (1984) Cultivation of banana using plantlets from meristem culture. HortScience 19 (2): 231–233

    Google Scholar 

  • Ichihashi S, Kako S (1977) Studies on clonal propagation of Cattleya through tissue culture method. II. Browning of Cattleya. J Jpn Soc Hortic Sei 46: 325–330

    Google Scholar 

  • Inouye N (1983) Effect of antiserum treatment on the production of virus-free Cymbidium by means of meristem culture. Nogaku Kenkyu 60: 123–133

    Google Scholar 

  • Jacoli GG (1978) Sequential degeneration of mycoplasma-like bodies in plant tissue cultures infected with aster yellows. Can J Bot 56: 133–140

    Google Scholar 

  • James DJ, Thurbon IJ (1979) Rapid in vitro rooting of the apple rootstock M9J. HortScience 54: 309–311

    Google Scholar 

  • Jarret RL, Fernandez ZR, Salazar S (1986) In vitro conservation at CATIE. Plant Genet Resour Newsl 68: 6–10

    Google Scholar 

  • Jordan M, Corters I, Montenegro G (1983) Regeneration of Lapageria rosea plantlets by tissue culture. Gartenbauwissenschaft 48: 97–100

    Google Scholar 

  • Kane ME, Sheehan TJ, Philman NL (1988) A micropropagation protocol using Fraser photinia for mutation induction and new cultivar selection. Proc Fla State Hortic Soc 100: 334–337

    Google Scholar 

  • Kartha KK (1986) Elimination of viruses from cultures in presence of antiviral chemicals. In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworth, London, pp 219–238

    Google Scholar 

  • Kartha KK, Gamborg OL (1975) Elimination of cassava mosaic disease by meristem culture. Phytopathology 65: 826–828

    Google Scholar 

  • Kartha KK, Leung N, Gamborg O (1979) Freeze-preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci Lett 15: 7–15

    Google Scholar 

  • Kartha KK, Leung N, Pahl K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J Am Soc Hortic Sci 105: 481–484

    CAS  Google Scholar 

  • Kartha KK, Pahl K, Leung NL, Mroginski LA (1981) Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea, and bean. Can J Bot 59: 1671–1679

    CAS  Google Scholar 

  • Kartha KK, Leung NL, Mroginski LA (1982) In vitro growth responses and plant regeneration from cryopreserved meristems of cassava (Manihot esculenta Crantz). Z Pflanzenphysiol 107: 133–140

    Google Scholar 

  • Kassanis B (1957) The use of tissue cultures to produce virus-free clones from infected potato varieties. Ann Appl Biol 45: 422–427

    Google Scholar 

  • Kassanis B, Varma A (1967) The production of virus-free clones of some British potato varieties. Ann Appl Biol 59: 447–450

    Google Scholar 

  • Kitto SL, Young MJ (1981) In vitro propagation of Carrizo citrange. HortScience 16: 305–306

    CAS  Google Scholar 

  • Knauss JF (1976) A tissue culture method for producing Dieffenbachia picta cv. Perfection free of fungi and bacteria. Proc Fla State Hortic Sci 89: 293–296

    Google Scholar 

  • Kromer K, Kukulczanka K (1985) In vitro cultures of meristem tips of Canna indica ( L. ). Acta Hortic 167: 279–285

    Google Scholar 

  • Kusey WE, Hammer PA, Weiler TC (1980) In vitro propagation of Gypsophila paniculala L. Bristol Fairy. HortScience 15: 600–601

    Google Scholar 

  • Lane WD (1979) In vitro propagation of Spirea bumalda and Prunus cistena from shoot apices. Can J Plant Sci 59: 1025–1029

    CAS  Google Scholar 

  • Lawson RH, Hearon SD (1973) Symtomatology of Cattleya mericlones infected with Cymbidium mosaic virus. Am Orchid Soc Bull 42: 1071–1074

    Google Scholar 

  • Leu L (1972) Freeing sugarcane from mosaic virus by apical meristem culture and tissue culture. Rep Taiwan Sugar Exp Stn 57: 57–63

    Google Scholar 

  • Limasset P, Cornuet P (1949) Recherche du virus de la mosaique du tabac dans les meristèmes des plantes infectées. CR Hebd Séances Acad Sci 228: 1971–1972

    Google Scholar 

  • Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia la tif o lia, by use of shoot-tip culture. Proc Int Plant Prop Soc 30: 421–427

    Google Scholar 

  • Long RD, Cassells AC (1986) In: Withers LA, Alderson (eds) Plant tissue culture and its agricultural applications. Butterworth, London, pp 239–248

    Google Scholar 

  • Lourens AG, Martin FA (1987) Evaluation of in vitro propagated sugarcane hybrids for somaclonal variation. Crop Sci 27 (4): 793–796

    Google Scholar 

  • Lundergan C, Janick J (1979) Low temperature storage of in vitro apple shoots. HortScience 14: 514

    Google Scholar 

  • Manzhulin AV, Butenko RG, Popov AS (1983) Effect of pretreatments of potato apices surviving after deep freezing. Sov Plant Physiol 30: 1188–1193

    CAS  Google Scholar 

  • Mater AA (1987) Production and cryogenic freezing of date palm germplasm and regeneration of plantlets from frozen material. Iraqi J Agric Sci 5: 35–49

    Google Scholar 

  • McComb JA, Newton S (1981) Propagation of kangaroo paws using tissue culture. J Hortic Sci 56: 181–183

    Google Scholar 

  • Mellor FC, Stace-Smith R (1969) Development of excised potato buds in nutrient medium. Can J Bot 47: 1617–1621

    Google Scholar 

  • Minocha SC (1980) Cell and tissue culture in the propagation of forest trees. In: Sala F, Parisi B, Cella R, Ciferri O (eds) Plant cell cultures: results and perspectives. Elsevier/North Holland Biomedical, Amsterdam, pp 295–300

    Google Scholar 

  • Monaco LC, Sondahl MR, Carvalho A, Crocomo OJ, Sharp WR (1977) Application of tissue culture in the improvement of coffee. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 109–129

    Google Scholar 

  • Monteuuis O (1987) In vitro meristem culture of juvenile and mature Sequoiadendron giganteum. Tree Physiol 3 (3): 265–272

    PubMed  Google Scholar 

  • Morel G (1960) Producing virus–free cymbidiums. Am Orchid Soc Bull 29: 495–497

    Google Scholar 

  • Morel G (1963) La culture in vitro du meristème apical de certaines orchidées. CR Hebd Séances Acad Sci 256: 4955–4957

    Google Scholar 

  • Morel G, Martin C (1952) Guérison de dahlias atteints d’une maladie à virus. CR Hebd Séances Acad Sei 235: 1324–1325

    CAS  Google Scholar 

  • Morel G, Martin C (1955) Guérison de pommes de terre atteintes de maladies à virus. CR Séances Acad AgricFr 41: 472–475

    Google Scholar 

  • Mori K, Hosokawa D (1977) Localization of viruses in apical meristem and production of virus-free plants by means of meristem and tissue culture. Acta Hortic 78: 389–396

    Google Scholar 

  • Mullins MG, Nair Y, Sampet P (1979) Rejuvenation in vitro: induction of juvenile characters in an adult clone of Vitis vinifera L. Ann Bot 44: 623–627

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    CAS  Google Scholar 

  • Oglevee-O’Donovan W (1986) Production of culture indexed geraniums. In: Zimmerman RH, Griesbach RJ, Hammerschlag FA, Lawson RH (eds) Tissue culture as a plant production system for horticultural crops. Martinus Nijhoff, Boston, pp 119–123

    Google Scholar 

  • Paludan N (1985) Inactivation of tobacco mosaic virus in Aeschynanthus hildebrandii by means of heat treatment, chemotherapy and meristem-tip culture. Tidsskr Planteavl 89: 273–278

    Google Scholar 

  • Papachatzi M, Hammer PA, Hasagawa PM (1981) In vitro propagation of Hosta decorata Thomas Hogg using cultured shoot tips. J Am Soc Hortic Sei 106: 232–236

    CAS  Google Scholar 

  • Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff, Boston, pp 296–300

    Google Scholar 

  • Pierik RLM (1988) Handicaps for the large scale commercial application of micropropagation. In Kozai T (ed) Acta Hortic Symp High technology in protected cultivation. Giken, Tokyo, pp 63–71

    Google Scholar 

  • Pillai SK, Hildebrandt AC (1968) Geranium plants differentiated in vitro from stem tip and callus cultures. Plant Dis Rep 52: 600–601

    Google Scholar 

  • Quak F (1977) Meristem culture and virus-free plants. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 598–615

    Google Scholar 

  • Reed BM (1990) Survival of in vitro-grown apical meristems of Pyrits following cryopreservation. HortScience 25 (1): 111–113

    Google Scholar 

  • Reed BM, Lagerstedt HB (1987) Freeze preservation of apical meristems of Rubiis in liquid nitrogen. HortScience 22 (2): 302–303

    CAS  Google Scholar 

  • Riviers S (1973) Culture in vitro du merestène apical du Lilium candidum L. ( Liliacées) apres ablation totale des primordiums foliaires. CR Acad Sei Ser D 1989–1992

    Google Scholar 

  • Robb SM (1957) The culture of excised tissue from bulb scales of Lilium speciosum Thun. J Exp Bot 8: 348–352

    Google Scholar 

  • Roggemans J, Claes MC (1979) Rapid clonal propagation of rhubarb by in vitro culture of shoot tips. Sei Hortic 11: 241–246

    CAS  Google Scholar 

  • Sakai A, Yamakawa M, Sakata D, Harada T, Yakuwa T (1978) Development of a whole plant from an excised strawberry runner apex frozen to -196 °C. Contrib Inst Low Temp Sei Ser Bull 36: 31–38

    Google Scholar 

  • Schnabdrauch LS, Sink KC (1979) In vitro propagation of Phlox subulata and Phlox paniculata. HortScience 14: 607–608

    Google Scholar 

  • Seibert M (1976) Shoot initiation from carnation shoot apices frozen to -196 °C. Science 191: 1178–1179

    PubMed  CAS  Google Scholar 

  • Shabde M, Murashige T (1977) Hormonal requirements of excised Dianthus caryophyllus L. shoot apical meristem in vitro. Am J Bot 64: 443–448

    CAS  Google Scholar 

  • Smith RH, Murashige T (1970) In vitro development of the isolated shoot apical meristem of angiosperms. Am J Bot 57: 562–568

    Google Scholar 

  • Snir I, Erez A (1980) In vitro propagation of Mailing Merton apple root stocks. HortScience 15: 597–598

    Google Scholar 

  • Stace-Smith R (1985) Virus-free clones through plant tissue culture. In: Robinson CW, Howel JA (eds) Comprehensive biotechnology Vol 4. The practice of biotechnology: specialty products and service activities. Pergamon Oxford, pp 169–179

    Google Scholar 

  • Stace-Smith R, Mellor FC (1968) Eradication of potato viruses X and S by thermotherapy and axillary bud culture. Phytopathology 58: 199–203

    Google Scholar 

  • Stevenson JH, Harris RE (1980) In vitro plantlet formation from shoot-tip expiants of Fuchsia hybrid cv. Swingtime. Can J Bot 58: 2190–2199

    Google Scholar 

  • Stone OM (1963) Factors affecting the growth of carnation plants from shoot apices. Ann Appl Biol 52: 199–209

    Google Scholar 

  • Sutter E, Langhams RW (1979) Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture. J Am Soc Hortic Sei 104: 493–496

    Google Scholar 

  • Takayama S, Misawa M (1980) Differentiation in Lilium bulbscales grown in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiol Plant 48: 121–125

    Google Scholar 

  • Ten Houten JG, Quak F, van der Meer FA (1968) Heat treatment and meristem culture for the production of virus-free plant material. Neth J Plant Pathol 74: 17–24

    Google Scholar 

  • Theiler R (1977) In vitro culture of shoot tips of Pelargonium species. Acta Hortic 78: 403–414

    Google Scholar 

  • Thimann KV (1977) Hormone action in the whole life of plants. Univ of Massachusetts Press, Amherst

    Google Scholar 

  • Towill LE (1981) Survival at low temperatures of shoot-tips from cultivars of Solanum tuberosum groupTuberosum. Cryo Lett 2: 373–382

    Google Scholar 

  • Towill LE (1988) Survived of shoot-tips from mint species after short-term exposure to cryogenic conditions. HortScience 23 (5): 839–841

    Google Scholar 

  • Uemura M, Sakai A (1980) Survival of carnation (Dianthus caryophyllus L.) shoot apices frozen to the temperature of liquid nitrogen. Plant Cell Physiol 21: 85–94

    CAS  Google Scholar 

  • Van Os H (1964) Production of virus-free carnations by means of meristem culture. Neth J Plant Pathol 70: 18–26

    Google Scholar 

  • Vine SJ, Jones OP (1969) The culture of shoot tips of hop (Humulus lupulus) to eliminate viruses. J Hortic Sci 44: 281–284

    Google Scholar 

  • Walkey DGA (1980) Production of virus-free plants by tissue culture. In: Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Blackwell Scientific, Oxford, pp 109–117

    Google Scholar 

  • Walkey DGA, Webb MJW (1968) Virus in plant apical meristems. J Gen Virol 3: 311–313

    Google Scholar 

  • Wang PJ (1977) Regeneration of virus-free potato from tissue culture. In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnological application. Springer, Berlin Heidelberg New York, pp 386–391

    Google Scholar 

  • Wang PJ (1978) Clonal multiplication of Cryptomeria japonica D. Don in vitro. In: Studies and essays in commemoration of the Golden Jubilee of Academia Sinica, Taipei, Taiwan, pp 559–566

    Google Scholar 

  • Wang PJ, Hu CY (1982) In vitro mass tuberization and virus-free seed–potato production in Taiwan. Am Potato J 59: 33–37

    Google Scholar 

  • Wang PJ, Hu CY (1984) In vitro cloning of the deciduous timber tree Sassafras randaiense. Z Pflanzenphysiol 113: 331–335

    CAS  Google Scholar 

  • Wang PJ, Hu CY (1985) Potato tissue culture and its application. In: Li PH (ed) Potato physiology. Academic Press, New York, pp 503–577

    Google Scholar 

  • Wang PJ, Huang LC (1974) Studies on the shoot meristem culture of Allium sativum. L. Chin Hortic 20: 79–87

    Google Scholar 

  • Wang PJ, Huang LC (1976) Beneficial effects of activated charcoal on plant tissue and organ cultures. In Vitro 12: 260–261

    Google Scholar 

  • White PR (1934) Multiplication of the viruses of tobacco and Aucuba mosaics in growing excised tomato root tips. Phytopathology 24: 1003–1011

    Google Scholar 

  • Wochok ZS, Sluis CJ (1980) Gibberellic acid promotes Atriplex shoot multiplication and elongation. Plant Sci Lett 17: 363–369

    CAS  Google Scholar 

  • Yakuwa H, Oka S (1988) Plant regeneration through meristem culture from vegetative buds of mulberry (Morus bombycis Koidz) stored in liquid nitrogen. Ann Bot 62: 79–82

    Google Scholar 

  • Yang YW, Hsing YI, Chang WC (1981) Clonal propagation oiStevia rebaudiana Bertoni through axillary shoot proliferation in vitro. Bot Bull Acad Sin 22: 57–62

    Google Scholar 

  • Zatyko JM, Molnar I (1985) Preliminary results on the in vitro mass propagation of grapes from shoot-tip meristem. Fruit Sci Rep 12: 83–85

    Google Scholar 

  • Ziv M (1979) Transplanting Gladiolus plants propagated in vitro. Sci Hortic 11: 257–260

    Google Scholar 

  • Ziv M, Halevy AH, Shilo R (1970) Organ and plantlet regeneration of Gladiolus through tissue culture. Ann Bot (Lond) 34: 671–676

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ball Floraplant, P.O. Box 335, West Chicago, IL, 60185, USA

    A. Charles

Authors
  1. P.-J. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Charles
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. A-137, New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Wang, PJ., Charles, A. (1991). Micropropagation Through Meristem Culture. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-76415-8_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76417-2

  • Online ISBN: 978-3-642-76415-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation