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High-Tech and Micropropagation I pp 495–515Cite as

Mass Propagation of Plants Through Shake- and Bioreactor-Culture Techniques

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Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE,volume 17)

Abstract

From the point of view of the industrial application of plant tissue culture, clonal propagation and secondary metabolite production are the most important fields of research (Damiano 1980, Fujita 1987, Sagawa 1989). Today, there are many commercial tissue culture nurseries throughout the world. Most of these nurseries use flasks or bottles containing agar medium for commercial propagation, but efficiency is not high because of labor and time costs. In order to improve efficiency, the use of shake culture techniques for the mass propagation of plants was previously reported (Takayama and Misawa 1982e). Recently, the author has tried to scale up the techniques using bioreactors (Takayama 1984,1986). Using a small bioreactor (4 to 201), over 4000 to 20 000 plantlets were produced within 1 to 2 months. The results are described here.

Keywords

  • Plant Tissue Culture
  • Mass Propagation
  • Draft Tube
  • Bioreactor Culture
  • Schematic Outline

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Author information

Authors and Affiliations

  1. Department of Biological Science and Technology, Tokai University, 317 Nishino, Numazu, Shizuoka, 410-03, Japan

    S. Takayama

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  1. S. Takayama
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Editors and Affiliations

  1. A-137, New Friends Colony, 110065, New Delhi, India

    Professor Dr. Y. P. S. Bajaj

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© 1991 Springer-Verlag Berlin Heidelberg

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Takayama, S. (1991). Mass Propagation of Plants Through Shake- and Bioreactor-Culture Techniques. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_27

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  • DOI: https://doi.org/10.1007/978-3-642-76415-8_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-76417-2

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