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Mass Propagation of Plants Through Shake- and Bioreactor-Culture Techniques

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Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE,volume 17)

Abstract

From the point of view of the industrial application of plant tissue culture, clonal propagation and secondary metabolite production are the most important fields of research (Damiano 1980, Fujita 1987, Sagawa 1989). Today, there are many commercial tissue culture nurseries throughout the world. Most of these nurseries use flasks or bottles containing agar medium for commercial propagation, but efficiency is not high because of labor and time costs. In order to improve efficiency, the use of shake culture techniques for the mass propagation of plants was previously reported (Takayama and Misawa 1982e). Recently, the author has tried to scale up the techniques using bioreactors (Takayama 1984,1986). Using a small bioreactor (4 to 201), over 4000 to 20 000 plantlets were produced within 1 to 2 months. The results are described here.

Keywords

  • Plant Tissue Culture
  • Mass Propagation
  • Draft Tube
  • Bioreactor Culture
  • Schematic Outline

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Akita M, Takayama S (1988) Mass propagation of potato tubers using jar fermentor techniques. Acta Hortic 230: 55–61

    Google Scholar 

  • Bajaj YPS, Sidhu MMS, Gill APS (1982) Some factors affecting the in vitro propagation of gladiolus. Sci Hortic 18: 269–275

    CrossRef  Google Scholar 

  • Damiano C (1980) Planning and building a tissue culture laboratory. In: Proc Conference on Nursery Production of Fruit Plants Through Tissue Culture-Applications and Feasibility. USD A, Beltville, pp 93–101

    Google Scholar 

  • Fujita Y, Tabata M (1987) Secondary metabolites from plant cells. Pharmaceutical applications and progress in commercial production. In: Green C et al. (ed) Plant Tissue and Cell Culture. Alan R Liss, New York, pp 169– 185

    Google Scholar 

  • Hartmann HT, Kester DE (1975) Plant propagation: principles and practices, 3rd edn. Prentice-Hall, Englewood Cliffs, NJ

    Google Scholar 

  • Hussey G (1975) Propagation of Hyacinthus by tissue culture. Sci Hortic 3: 21–28

    CrossRef  Google Scholar 

  • Hussey G (1977) In vitro propagation of some members of the Liliaceae, Iridaceae and Amaryllidaceae. Acta Hortic 78: 303–309

    Google Scholar 

  • Ikeda H (1985) Culture vessel for photoautotrophic culture. Japanese Patent, Kokai Tokyo Koho 60-237984 (in Japanese)

    Google Scholar 

  • Inoue M (1984) Culture instrument for photoautotrophic organisms. Japanese Patent, Kokai 59–21682

    Google Scholar 

  • Mii M, Mori T, Iwase N (1974) Organ formation from excised bulb scales of Hyppeastrum hybridum in vitro. J Hortic Sci 49: 241–244

    CAS  Google Scholar 

  • Miwa Y (1989) Robot in tissue culture nursery (in Japanese). In: ICMSA Committee (eds) Measurement, control and information in production of plants. Keisoku jidou Seigyo Gakkai, Tokyo, pp 202–210

    Google Scholar 

  • Nagaoka M (1989) Bio-Nursery System. In: Takatsuji M (ed) Syokubutu Seisan Sisutemu Jituyou Jiten. Fuji Technosystem, Tokyo, pp 1271–1272

    Google Scholar 

  • Paek KY, Thorpe TA (1989) Hyacinth. In: Ammirato PV, Evans DA, Sharp RW, Bajaj YPS (eds) Handbook of plant cell culture Vol 5. McGraw-Hill, New York, pp 479–508

    Google Scholar 

  • Sagawa Y, Kunisaki JT (1989) Micropropagation of floricultural crops. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, Vol 5. McGraw-Hill, New York, pp 25–56

    Google Scholar 

  • Styer DJ (1985) Bioreactor technology for plant propagation. In: Henke RR, Hughes KW (eds) Tissue culture in forestry and agriculture. Plenum, New York, pp 117–130

    Google Scholar 

  • Takada T, Oota Y (1988) Culture of plant cells under illumination. In: Plant tissue culture for bioengineering. The Japanese Association for Plant Tissue and Cell Culture, Tokyo, pp 94–95

    Google Scholar 

  • Takatsu I (1978) Practical methods for meristem culture of gladiolus and the production of virus-free plants (in Japanese). Agric Hortic 53: 797–800

    Google Scholar 

  • Takayama S (1984) Mass propagation of plants through shake culture techniques. Plant Tissue Cult Lett 1: 8–13

    CrossRef  Google Scholar 

  • Takayama S (1986) Mass propagation of plants by fermentor culture techniques, Abst VI Intern Congr Plant Tissue and Cell Culture, Minnesota University, pp 449

    Google Scholar 

  • Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiol Plant 46: 184–190

    Google Scholar 

  • Takayama S, Misawa M (1980) Differentiation in Lilium bulbscales grown in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro. Physiol Plant 48: 121–125

    Google Scholar 

  • Takayama S, Misawa M (1981) Mass propagation of Begonia * hiemalis plantlets by shake culture. Plant Cell Physiol 22: 461–467

    CAS  Google Scholar 

  • Takayama S, Misawa M (1982a) Factors affecting differentiation and growth in vitro, and a mass propagation scheme for Begonia x hiemalis. Sci Hortic 16: 65–75

    CrossRef  CAS  Google Scholar 

  • Takayama S, Misawa M (1982b) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23: 67–74

    CAS  Google Scholar 

  • Takayama S, Misawa M (1982c) A scheme for mass propagation of Lilium in vitro. Sei Hortic 18: 353–362

    CrossRef  Google Scholar 

  • Takayama S, Misawa M (1982d) Cultivation of in vitro propagated Lilium bulbs in soil. J Am Soc Hortic Sei 107: 830–834

    Google Scholar 

  • Takayama S, Misawa M (1982e) Mass propagation of ornamental plants through tissue culture. In: Fujiwara A (ed) Plant Tissue Culture 1982, Maruzen, Tokyo, pp 681–682

    Google Scholar 

  • Takayama S, Misawa M (1983) The mass propagation of Lilium in vitro through stimulation of multiple adventitious bulbscale formation and through shake culture. Can J Bot 61: 224–228

    CrossRef  Google Scholar 

  • Takayama S, Arno T, Fukano M, Oosawa K (1985a) Mass propagation of strawberries by jar fermentor culture. (1) Establishment of aseptic culture and the long-term storage conditions in vitro under low temperature (in Japanese). Abst 1985 Spring Meeting of Japanese Society for Horticultural Science, Tokyo, April 3,1985, Japanese Society for Horticultural Science, pp 218–219

    Google Scholar 

  • Takayama S, Arno T, Fukano M, Oosawa K (1985b) Mass propagation of strawberries by jar fermentor culture. (2) Studies on the optimum conditions in a liquid medium and the establishment of mass propagation scheme using a jar fermentor (in Japanese). Abst 1985 Spring Meeting of Japanese Society for Horticultural Science, Tokyo, April 3,1985, Japanese Society for Horticultural Science, pp 220–221

    Google Scholar 

  • Takayama S, Arno T, Fukano M, Akita M (1987) Mass propagation of gladiolus by liquid culture methods (in Japanese). Abst 10th Meeting Japanese Assoc Plant Tissue Culture, Sendai, July 14, 1987, Japanese Association for Plant Tissue Culture, 88 pp

    Google Scholar 

  • Takayama S, Arno T, Fukano M (1991) Rapid clonal propagation of Hyacinthus orientalis bulbs by shake culture. Scientia Hortic 45: 315–321

    CrossRef  Google Scholar 

  • Tamura S (1978) Adventitious bud formation from excised hyacinth bulbscale in vitro. J Jpn Soc Hortic Sei 46: 501–508

    CrossRef  Google Scholar 

  • Tanaka H, Nishijima F, Suwa M, Iwamoto T (1983) Rotating drum fermentor for plant cell suspension cultures. Biotechnol Bioeng 25: 2359–2370

    PubMed  CrossRef  CAS  Google Scholar 

  • Ushiyama K (1988) Mass culture technology for plant cells and the production of secondary metabolites (In Japanese). Bioscience and Industry 46: 7–11

    Google Scholar 

  • Ushiyama K, Miyamoto Y, Oda H, Ishida Y (1984a) Gaseous phase culture equipment for continuous culture of plant tissues (in Japanese). Japanese Patent, Kokai Tokyo Koho, 59–45873

    Google Scholar 

  • Ushiyama K, Oda H, Miyamoto Y, Ishida Y (1984b) Gaseous phase culture method and the equipment for plant tissues. Japanese Patent, Kokai Tokyo Koho, 59–45879, (in Japanese)

    Google Scholar 

  • Ushiyama K, Oda H, Miyamoto Y (1986) Large-scale tissue culture of Panax ginseng root. Abstr VI Intern Congr Plant Tissue and Cell Culture, Minnesota University, pp 252

    Google Scholar 

  • Ushiyama K (1988) Large scale culture techniques of plant cell and the production of secondary metabolites. BioSci Industry 46: 7–11

    Google Scholar 

  • Wagner F, Vogelmann H (1977) Cultivation of plant tissue in bioreactors and formation of secondary metabolites. In: Barz W, Reinhard E, Zenk MH (eds) Plant formation of secondary metabolites. Plant tissue culture and its bio-technological application. Springer, Berlin Heidelberg New York, pp 245–252

    CrossRef  Google Scholar 

  • Yanagawa T, Sakanishi H (1977) Regeneration of bulblets on Hippeastrum hybridum bulb segments excised from various parts of a parent bulb. J Jpn Soc Hort Sei 46: 250–260

    CrossRef  Google Scholar 

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© 1991 Springer-Verlag Berlin Heidelberg

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Takayama, S. (1991). Mass Propagation of Plants Through Shake- and Bioreactor-Culture Techniques. In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation I. Biotechnology in Agriculture and Forestry, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76415-8_27

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  • DOI: https://doi.org/10.1007/978-3-642-76415-8_27

  • Publisher Name: Springer, Berlin, Heidelberg

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