Problems with Explant Exudation in Micropropagation

  • J. E. Preece
  • M. E. Compton
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 17)


During the time of organ establishment for the purpose of micropropagation, discoloration of the medium and browning or blackening of the explant is often observed. This may be a sign of potential problems and may mean that special techniques will be required to obtain a healthy-looking and rapidly growing explant. Similar staining of the medium may be observed throughout the in vitro cycle. Although this is a concern for those involved in micropropagation, it has also been noted by researchers working with protoplasts, callus, somatic embryos, and other forms of plant tissue culture.


Shikimic Acid Plant Cell Tissue Organ Cult Stock Plant Black Walnut Tobacco Callus 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • J. E. Preece
  • M. E. Compton
    • 1
  1. 1.Department of Plant and Soil ScienceSouthern Illinois UniversityCarbondaleUSA

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