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Stains-All is a Dye that Probes the Conformational Features of Calcium Binding Proteins

  • Yogendra Sharma
  • Doraivajan Balasubramanian

Abstract

The field of calcium-binding proteins (CaBP) is still growing rapidly with increasing discoveries of these proteins in a variety of tissues. Significant methodological progress has been made in identifying calcium-binding proteins (CaBP) and in investigating their physicochemical and biochemical properties. The usual methods for the detection and identification of calcium-binding proteins used are ultrafiltration, 45Ca overlay in gel electrophoresis and subsequent autoradiography, Chelex competitive calcium binding assay, and use of lanthanum luminescence, particularly of terbium, as calcium mimic. Analysis of calcium binding properties implies various methods such as equilibrium dialysis or flow dialysis, equilibrium gel filtration, spectroscopic procedures, and others (for a review, see Schachtele and Marme 1988). Considering the general interest in calcium and CaBP, we review here the less described but widely used technique of staining using the cationic dye called Stains-all.

Keywords

Calcium Binding Malignant Hyperthermia Polyaspartic Acid Cardiac Sarcoplasmic Reticulum Sarcoplasmic Reticulum Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Yogendra Sharma
  • Doraivajan Balasubramanian

There are no affiliations available

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