Abstract
Efficient in vitro DNA amplification depends on optimizing several parameters one of which is the selection of the primer sequences, In many cases primers will do their job. However, to minimize unwanted polymerase reactions it is advisable to regard the rules given by some experimentators [1,2].
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References
Saiki RK (1989) The Design and Optimization of the PCR. In: Erlich HA (ed) PCR Technology, Stockton Press, New York, London, p 7
Rappolee DA (1990) Optimizing the Sensitivity of RT-PCR. Amplifications — A Forum for PCR Users, No. 4, Perkin Elmer Cetus, Norwalk, CT, p 5
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© 1991 Springer-Verlag Berlin Heidelberg
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Röllich, G., Jafari, M. (1991). Computer Program for the Selection of PCR Primers. In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_7
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DOI: https://doi.org/10.1007/978-3-642-75924-6_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52934-7
Online ISBN: 978-3-642-75924-6
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