PCR Topics pp 203-205 | Cite as

Detection of Chlamydia Trachomatis by PCR

  • M. C. Petit
  • M. H. Francois
  • S. Berriche
  • A. Burckel
  • G. Lucotte

Abstract

Chlamydia trachomatis, an obligate intracellular parasite, is a common agent of sexually transmitted diseases, and the causative agent of several human clinical conditions including urethritis, cervititis, salpingitis, female infertility, lymphogranuloma venereum and trachoma. The diagnosis of C. trachomatis as a pathogen is currently difficult and expensive, because laboratory diagnosis is achieved by isolation in cell culture, a technique which is both cumbersome and time-consuming.

Keywords

EDTA Agarose Bromide Electrophoresis Gelatin 

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References

  1. 1.
    Dutilh B, Bebear C, Rodriguez P, Vekris A, Bonnet J, Garret M (1989). Specific amplification of a DNA sequence common to all Chlamydia trachomatis serovars using the polymerase chain reaction. Res Microbiol 140: 7–16PubMedCrossRefGoogle Scholar
  2. 2.
    Comanducci M, Ricci S, Ratti G (1988). The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells. Mol Microbiol 2: 531–538PubMedCrossRefGoogle Scholar
  3. 3.
    Griffais R, Thibon M (1989). Detection of Chlamydia trachomatis by the polymerase chain reaction. Res microbiol 140: 139–141PubMedCrossRefGoogle Scholar
  4. 4.
    Hatt C, Ward ME, Clarke IN (1988). Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1 evidence for involvement in DNA replication. NUCL Acids Res 16: 4053–4067PubMedCrossRefGoogle Scholar
  5. 5.
    Palmer L, Falfow S (1986). A common plasmid of the genus Chlamydia trachomatis. Plasmid 16: 52–62PubMedCrossRefGoogle Scholar
  6. 6.
    Pollard DR, Tyler SD, Ngo CW, Rozee KR (1989). A polymerase chain reaction (PCR) protocol for the specific detection of Chlamydia spp. Mol Cell Probes 3: 383–389PubMedCrossRefGoogle Scholar
  7. 7.
    Ripa KV, Mardh PA (1977). Cultivation of Chlamydia trachomatis in cyclohexamidetreated Mc Coy cells. J Clin Microbiol 6: 329–331Google Scholar
  8. 8.
    Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Ehrlich HA (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491PubMedCrossRefGoogle Scholar
  9. 9.
    Schachter J, Martin DH (1987). Failure of multiples passages to increase chlamydial recovery. J Clin Microbiol 25: 1851–1853PubMedGoogle Scholar
  10. 10.
    Sriprakash KS, Mac Avoy ES (1987). Characterisation and sequence of a plasmid from the trachoma biovar of Chlamydia trachomatis. Plasmid 18: 205–214PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • M. C. Petit
  • M. H. Francois
  • S. Berriche
  • A. Burckel
  • G. Lucotte

There are no affiliations available

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