Abstract
Molecular classification and identification of bacteria as well as the development of specific DNA probes is a tedious procedure often requiring multiple cloning steps. We demonstrate here a universal and general strategy which does not require any cloning steps suitable I) for inferring phylogenetic relationships, II) for characterization and definition of taxa at the molecular level and III) for the development of specific DNA probes. A target molecule fitted for such purposes should I) be present in all cellular organisms, II) consist of conserved and variable regions, III) contain a high information content and IV) be functionally constrained. Besides conserved proteins, e.g. cytochromes and heat-shock proteins, other conserved structures like ribosomal RNAs meet these conditions.
Keywords
- Comparative Sequence Analysis
- Small Subunit rRNAs
- Mycobacterial Species
- High Information Content
- Tedious Procedure
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Böttger EC (1989) Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol. Lett. 65: 171–176
Dams E, Hendriks L, Van de Peer Y, Neefs JM, Smits G, Vandenbempt I, De Wachter R (1988) Compilation of small subunit ribosomal RNA sequences. Nucl. Acids. Res. 16 (suppl.) r87–r175
Edwards U, Rogall T, Blöcker H, Emde M, Böttger, EC (1989) Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucl. Acids Res. 17: 7843–7853
Gray MW, Sankoff D, Cedargreen RJ (1984) On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved core structure in small subunit ribosomal RNA. Nucl. Acids. Res. 12: 5837–5852
Woese CR (1987) Bacterial Evolution. Microbiol. Rev. 51: 221–271
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Böddinghaus, B., Rogall, T., Flohr, T., Blöcker, H., Wolters, J., Böttger, E.C. (1991). Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes. In: Rolfs, A., Schumacher, H.C., Marx, P. (eds) PCR Topics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75924-6_16
Download citation
DOI: https://doi.org/10.1007/978-3-642-75924-6_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52934-7
Online ISBN: 978-3-642-75924-6
eBook Packages: Springer Book Archive