The Transforming Activity of PP59C-MYCis Weaker Than That of v-myc
We and others have speculated that elevated expression of c-myc plays an important role in the establishment of the transformed follicle, a preneoplastic lesion essential for the development of the ALV-induced bursal-dependent lymphoma (Baba and Humphries 1985, Clurman and Hayward 1989, Neiman et al 1985). The discovery of base substitutions and missense mutations in some tumor c- myc alleles raises the possibility that qualitative as well as quantitative alterations within the chicken c-myc proto-oncogene may play a role in the genesis and/or progression of bursal lymphomas (Westaway et al 1984). In order to assess the contribution that deregulated expression of an unaltered c-myc allele makes during ALV lymphomagenesis (Hayward et al 1981) , we have constructed a recombinant avian retrovirus, SNVc-myc, that encodes the second and third exons of normal chicken c-myc and expresses only the smaller of the two c-myc proto-oncogene products, pp62c-myc and pp59c-myc (Hann et al 1988). Ultimately, this virus will enable the effects of specific mutations within the context of wild-type pp59c-myc to be assessed.
KeywordsHerpes Simplex Virus Thymidine Kinase Simplex Virus Thymidine Kinase Gene Reticuloendotheliosis Virus Spleen Necrosis Virus Peripheral Blood Cell Culture
Unable to display preview. Download preview PDF.
- Martin P, Henry C, Ferre F, Duterque-Coquillaud M, Lagrou C, Ghysdael J, Debuire B, Stehelin D and Saule S (1986) Transformation of quail embryo fibroblasts by a retrovirus carrying a normal human c-myc gene. Eur Mol Biol Org Jrnl 5:1529–1533Google Scholar
- Watson DK, Premkumar Reddy E, Duesberg PH and Papas TS (1983) Nucleotide sequence analysis of the chicken c-myc gene reveals homologous and unique coding regions by comparison with the transforming gene of avian myelocytomatosis virus MC29, Δgag-myc*. Proc Natl Acad Sci USA 80:2146–2150PubMedCrossRefGoogle Scholar